A systematic search for potential substrates of deubiquitinase with quantitative proteomics
Project/Area Number |
21710200
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Applied genomics
|
Research Institution | The University of Tokyo |
Principal Investigator |
OTA Kazuhisa The University of Tokyo, 大学院・理学系研究科, 助教 (00322727)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 翻訳後修飾 / ユビキチン / 出芽酵母 / プロテオミクス / 質量分析 |
Research Abstract |
Combining our originally poly-ubiquitinated protein enrichment method and high throughput LC-MS/MS analysis, we tried to identify specific substrates for all USP type deubiquitinases coded in the genome of Saccharomyces cerevisiae. Finally, we identified 94 potential substrate-enzyme relationships and, for some of these, made validation with molecular biological methods.
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Report
(3 results)
Research Products
(11 results)