| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
DNA methylation of cytosine is known to be one of the signals for cancer. The detection and quantification of methylcytosines have the possibility of leading to genetic diagnosis technology for prediction of cancer. Bisulfite sequencing is very famous method for detecting methylcytosines, which has problem in quantity of final methylation because of the reaction of DNA with high concentration of bisulfite at higher acidity and higher temperature. To develop the quantification, milder reaction was performed on DNA with low concentrations of reagents at lower acidity and lower temperature.
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