Development of Quantitative Bisulfite Sequencing
Project/Area Number |
21710201
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Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Applied genomics
|
Research Institution | University of Toyama |
Principal Investigator |
OKA Yoshimi University of Toyama, 事務局, 特命助教 (30470115)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 核酸 / 癌 / ゲノム |
Research Abstract |
DNA methylation of cytosine is known to be one of the signals for cancer. The detection and quantification of methylcytosines have the possibility of leading to genetic diagnosis technology for prediction of cancer. Bisulfite sequencing is very famous method for detecting methylcytosines, which has problem in quantity of final methylation because of the reaction of DNA with high concentration of bisulfite at higher acidity and higher temperature. To develop the quantification, milder reaction was performed on DNA with low concentrations of reagents at lower acidity and lower temperature.
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Report
(3 results)
Research Products
(10 results)