| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Research Abstract |
To identify possible operon rule, glycolytic genes of Escherichia coli were assembled in multiple types of polycistronic operons that have variety in gene connection order. Through evaluation of functionality by growth and gene expression analysis, a feature of operon in which gene expression profile is downward trend from promoter-proximal to -distal position was revealed. Arrangement of genes according to expression strength of the genes in wild type gave an operon with fastest growth in tested.
|
