Proteomic analysis of anhydrobiotic and extremotolerant animal

• Project/Area Number: 21710223
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: The University of Tokyo
• Principal Investigator: 國枝 武和 The University of Tokyo, 大学院・理学系研究科, 助教 (10463879)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: プロテオミクス / クマムシ / 極限環境耐性 / 乾眠 / 放射線耐性 / クロマチン / 抗凝集性 / 円偏光二色性 / タンパク質 / プロテオーム / 蛋白質 / 動物 / 親水性

Research Abstract

We identified several novel anti-aggregation proteins from an anhydrobiotic tardigrade, Ramazzottius varieornatus and revealed that these proteins were expressed abundantly even in active state. Although these proteins were found in less-tolerant taridirades, there are some correlation between their denaturing temperature and desiccation-tolerance. Our identified proteins are good basis to elucidate the molecular mechanism of anhydrobiotic ability and extremotolerance of tardigrades.

Research Products

• [Presentation] 極限環境耐性動物クマムシのゲノム解析と機能プロテオミクス (2011)
  • Author(s): 國枝武和
  • Organizer: 沖縄知的クラスター形成シンポジュウム(招待講演)
  • Place of Presentation: 那覇
  • Year and Date: 2011-01-29
• [Presentation] 極限環境耐性動物クマムシのゲノム解析と機能プロテオミクス (2011)
  • Author(s): 國枝武和
  • Organizer: 沖縄知的クラスター形成シンポジュウム
  • Place of Presentation: 那覇沖縄産業支援センター
  • Year and Date: 2011-01-29
• [Presentation] 極限環境耐性動物クマムシの分子生物学的解析基盤の創成 (2010)
  • Author(s): 國枝武和
  • Organizer: 第11回極限環境生物学会(奨励賞受賞講演)
  • Place of Presentation: 京都
  • Year and Date: 2010-11-16
• [Presentation] 極限環境耐性動物クマムシの分子生物学的解析基盤の創成 (2010)
  • Author(s): 國枝武和
  • Organizer: 第11回極限環境生物学会
  • Place of Presentation: 京都(奨励賞受賞講演)
  • Year and Date: 2010-11-16
• [Presentation] 極限環境耐性動物ヨコヅナクマムシ由来の抗凝集性タンパク質の解析 (2010)
  • Author(s): 國枝武和
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] 極限環境耐性動物ヨコヅナクマムシ由来の抗凝集性タンパク質の解析 (2010)
  • Author(s): 國枝武和
  • Organizer: 日本動物学会 第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] Genome analysis and functional proteomics of anhydrobiotic extremotolerant tardigrade, Ramazzottius cf. varieornatus. (2009)
  • Author(s): 國枝武和
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Genome analysis and functional proteomics of anhydrobiotic extremotolerant tardigrade, Ramazzottius cf.varieornatus (2009)
  • Author(s): 國枝武和
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] The analysis of anti-aggregation proteins from tardigrades with desiccation tolerance. (2009)
  • Author(s): 山口理美
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] The analysis of anti-aggregation proteins from tardigrades with desiccation tolerance (2009)
  • Author(s): 山口理美
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] 乾燥耐性を持つクマムシ由来の抗凝集性タンパク質の解析 (2009)
  • Author(s): 山口理美
  • Organizer: 第10回極限環境微生物学会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-28
• [Remarks] ホームページ等
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/saibou/kuma/
• [Remarks] アウトリーチ活動
• [Remarks] 爆笑問題のニッポンの教養(2010年8月3日、NHK総合)
• [Remarks] ミニゲノムひろば(2009年12月10日、福岡)
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/saibou/kuma/