Identification and characterization of unique radical SAM enzymes, which use S-adenosyl methionine as oxidant

• Project/Area Number: 21710224
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: KUDO Fumitaka Tokyo Institute of Technology, 大学院・理工学研究科, 准教授 (00361783)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 生合成 / 酵素化学 / ラジカルSAM酵素 / 二次代謝遺伝子 / 放線菌 / ネオマイシン / ゲンタミシン / カルバペネム抗生物質 / ラジカルSAMタンパク質 / 二次代謝遺伝子クラスター / ラジカルSAM異性化酵素 / カルバペネム / ドラフトゲノム解析 / トブラマイシン / デオキシ化酵素

Research Abstract

Identification of new radical SAM enzyme gene was found to be an efficient strategy to identify novel secondary metabolic biosynthetic gene cluster. Further, a novel radical SAM epimerase involved in neomycin biosynthesis was characterized.

Research Products

• [Journal Article] Biosynthetic genes for aminoglycoside antibiotics (2009)
  • Author(s): F.Kudo, T.Eguchi
  • Journal Title: Journal of Antibiotics 62 Pages: 471-481
  • NAID: 10030636178
  • Peer Reviewed
• [Journal Article] Enzymatic Preparation of Neomycin C from Ribostamycin (2009)
  • Author(s): F.Kudo, T.Kawashima, K.Yokoyama, T.Eguchi
  • Journal Title: Journal of Antibiotics 62 Pages: 643-646
  • NAID: 10030636780
  • Peer Reviewed
• [Journal Article] Enzymatic activity of a glycosyltransferase KanM2 encoded in the kanamycin biosynthetic gene cluster (2009)
  • Author(s): F.Kudo, H.Sucipto, T.Eguchi
  • Journal Title: Journal of Antibiotics 62 Pages: 707-710
  • NAID: 10030637136
  • Peer Reviewed
• [Presentation] Functional analysis of kanamycin biosynthesis enzymes (2010)
  • Author(s): Hilda Sucipto, Fumiaka Kudo, Tadashi Eguch
  • Organizer: International Chemical Congress of Pacific Basin Societies, Biosynthesis of Natural Products
  • Place of Presentation: Honolulu, HI(USA)
  • Year and Date: 2010-12-17
• [Presentation] Functional analysis of kanamycin biosynthesis enzymes (2010)
  • Author(s): H.Sucipto, F.Kudo, T.Eguchi
  • Organizer: International Chemical Congress of Pacific Basin Societies, Biosynthesis of Natural Products
  • Place of Presentation: Honolulu, HI (USA)
  • Year and Date: 2010-12-17
• [Presentation] アミノ配糖体抗生物質ネオマイシンCの酵素的調製 (2010)
  • Author(s): 川島大輝・横山健一・工藤史貴・江口正
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学
  • Year and Date: 2010-03-27
• [Presentation] アミノ配糖体抗生物質ネオマイシンCの全生合成の解明 (2009)
  • Author(s): 工藤史貴、横山健一、木下俊佑、山本恭士、藤井拓也、天貝啓太、川島大輝、江口正
  • Organizer: 第51回天然有機化合物討論会
  • Place of Presentation: 名古屋市公会堂
  • Year and Date: 2009-10-07
• [Remarks] ホームページ
  • URL: http://www.cms.titech.ac.jp/~eguchi/
• [Remarks]
  • URL: http://www.cms.titech.ac.jp/~eguchi/
• [Remarks]
  • URL: http://www.cms.titech.ac.jp/~eguchi/