Identification and characterization of unique radical SAM enzymes, which use S-adenosyl methionine as oxidant
Project/Area Number |
21710224
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Living organism molecular science
|
Research Institution | Tokyo Institute of Technology |
Principal Investigator |
KUDO Fumitaka Tokyo Institute of Technology, 大学院・理工学研究科, 准教授 (00361783)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 生合成 / 酵素化学 / ラジカルSAM酵素 / 二次代謝遺伝子 / 放線菌 / ネオマイシン / ゲンタミシン / カルバペネム抗生物質 / ラジカルSAMタンパク質 / 二次代謝遺伝子クラスター / ラジカルSAM異性化酵素 / カルバペネム / ドラフトゲノム解析 / トブラマイシン / デオキシ化酵素 |
Research Abstract |
Identification of new radical SAM enzyme gene was found to be an efficient strategy to identify novel secondary metabolic biosynthetic gene cluster. Further, a novel radical SAM epimerase involved in neomycin biosynthesis was characterized.
|
Report
(3 results)
Research Products
(10 results)