| Project/Area Number |
21710226
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyoto University |
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Principal Investigator |
MIYASHITA Masahiro Kyoto University, 大学院・農学研究科, 助教 (80324664)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ケミカルバイオロジー / ペプチド / 植物免疫 / 生分解 / ファイトアレキシン / 過酸化水素 / 受容体 / マレイミド基 / ヨウ素化 |
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| Research Abstract |
Plants defend themselves using an innate immune system, which is activated in response to a variety of elicitor molecules. Previously, we discovered a novel elicitor peptide (YGIHTH-amide, PIP-1) from a combinatorial random library. In this study, we first evaluated the effect of degradation of PIP-1 on defense responses and found that continuous activation is required for phytoalexin biosynthesis. Next, we synthesized a PIP-1 analog, in which the His6 residue is substituted with Cys, to obtain a labeled analog for characterization of its receptor. Modification at the Cys residue using iodophenylmaleimide had no effect on activity, indicating that this analog can be used as a radiolabeled ligand.
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