| Project/Area Number |
21710228
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMADA Hisatsugu Kyoto University, 先端医工学研究ユニット, 助教 (80512764)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 機能性核酸 / 光増感反応 / DNA内電荷移動反応 / 5-メチルシトシン / 同位体効果 / DNA内電荷移動 / DNAメチル化 / 修飾シトシン塩基 / 重水素同位体効果 |
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| Research Abstract |
Our interest focuses on the potential application of remarkable photooxidative reactivity of 5-methylcytosine (^mC) to methylation-mapping system and on further understanding the mechanism of photooxidation at ^mC in DNA. In the project, we revealed that (1) incorporation of an anthraquinone (AQ) sensitizer into a DNA strand enhanced one-electron photooxidation, leading to efficient strand cleavage at the ^mC site, (2) deprotonation at the C5-methyl group of an intermediate ^mC radical cation may occur as a key elementary reaction in the AQ-photooxidative strand cleavage at the ^mC, and (3) DNA strand cleavage at multiple ^mC residues was induced by AQ-photoinjected positive charge (hole) transfer through DNA duplex. The results obtained may provide a guide for the molecular design of highly sensitive and selective photochemical methods for the identification and analysis of ^mC in DNA.
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