Measurement of nonequilibrium fluctuations of molecular motor F1-ATPase

• Project/Area Number: 21740291
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Mathematical physics/Fundamental condensed matter physics
• Research Institution: Chuo University
• Principal Investigator: TOYABE Shoichi Chuo University, 理工学部, 助教 (40453675)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 非平衡 / 非線形物理学 / 分子モーター / 生物物理学 / 非平衡揺らぎ / 非線形応答 / 回転電場法 / F1-ATPase

Research Abstract

In order to reveal the energetics of rotary molecular motor F1-ATPase, we measured the response of F1-ATPase against a constant and periodic torque by using an electrorotation method. We found that F1-ATPase can convert the chemical free energy change of ATP hydrolysis to mechanical motion.

Research Products

• [Journal Article] Experimental demonstration of information-to-energy conversion and validation of the generalized Jarzynski equality (2010)
  • Author(s): Shoichi Toyabe, Takahiro Sagawa, Masahito Ueda, Eiro Muneyuki, Masaki Sano
  • Journal Title: Nature Physics 6 Pages: 988-988
  • Peer Reviewed
• [Journal Article] Nonequilibrium energetics of a single F_1-ATPase molecule (2010)
  • Author(s): Shoichi Toyabe, Tetsuaki Okamoto, Takahiro Watanabe-Nakayama, Hiroshi Taketani, Seishi Kudo, Eiro Muneyuki
  • Journal Title: Phys.Rev.Lett. 104 Pages: 198103-198103
  • Peer Reviewed
• [Journal Article] Single-molecule nonequilibrium energetics of a molecular-motor F_1-ATPase (2010)
  • Author(s): 鳥谷部祥一, 宗行英朗
  • Journal Title: 物性研究 研究会報告 95 Pages: 320-320
• [Journal Article] Observation of DNA pinning at laser focal point on Au surface and its application to single DNA nanowire and cross-wire formation (2010)
  • Author(s): Sho Fujii, Katsuaki Kobayashi, Katsuhiko Kanaizuka, Tetsuaki Okamoto, Shoichi Toyabe, Eiro Muneyuki, Masa-aki Haga
  • Journal Title: Bioelectrochemistry 80 Pages: 26-26
  • Peer Reviewed
• [Journal Article] Manipulation of Single DNA Using a Micronanobubble Formed by Local Laser Heating on a Au-coated Surface (2010)
  • Author(s): Sho Fujii, Katsuaki Kobayashi, Katsuhiko Kanaizuka, Tetsuaki Okamoto, Shoichi Toyabe, Eiro Muneyuki, Masa-aki Haga
  • Journal Title: Chem.Lett. 39 Pages: 92-92
  • NAID: 10027176924
  • Peer Reviewed
• [Journal Article] Nonequilibrium energetics of a single F1-ATPase molecule (2010)
  • Author(s): Shoichi Toyabe
  • Journal Title: Physical Review Letters Volume: 104 Pages: 198103-198103
  • Peer Reviewed
• [Journal Article] Experimental demonstration of information-to-energy conversion and validation of the generalized Jarzynski equality (2010)
  • Author(s): Shoichi Toyabe
  • Journal Title: Nature Physics Volume: 6 Pages: 988-992
  • Peer Reviewed
• [Journal Article] Single-molecule nonequilibrium energetics of a molecular-motor F_1-ATPase (2010)
  • Author(s): 鳥谷部祥一
  • Journal Title: 物性研究 Volume: 95 Pages: 320-322
• [Journal Article] Manipulation of Single DNA Using a Micronanobubble Formed by Local Laser Heating on a Au-coated Surface (2010)
  • Author(s): S.Fujii
  • Journal Title: Chemistry Letters 39 Pages: 92-93
  • Peer Reviewed
• [Presentation] 1分子トラジェクトリから分子モーターのポテンシャルを推定する (2011)
  • Author(s): 鳥谷部祥一, 上野博史, 宗行英朗
  • Organizer: 日本物理学会
  • Place of Presentation: 新潟大学
  • Year and Date: 2011-03-26
• [Presentation] 情報-自由エネルギー変換と回転分子モーター (2011)
  • Author(s): 鳥谷部祥一
  • Organizer: 日本磁気学会スピンエレクトロニクス専門研究会「スピンと力学系」
  • Place of Presentation: 東京大学
  • Year and Date: 2011-01-07
• [Presentation] 情報を自由エネルギーに変換する (2010)
  • Author(s): 鳥谷部祥一
  • Organizer: 基研研究会「非平衡系の物理-非平衡ゆらぎと集団挙動」
  • Place of Presentation: 京都大学
  • Year and Date: 2010-11-18
• [Presentation] 非平衡フィードバック制御による情報-エネルギー変換 (2010)
  • Author(s): 鳥谷部祥一, 沙川貴大, 上田正仁, 宗行英朗, 佐野雅己
  • Organizer: 日本物理学会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2010-09-23
• [Presentation] Information-heat engine as a model system of molecular motors (2010)
  • Author(s): Shoichi Toyabe, Takahiro Sagawa, Masahito Ueda, Eiro Muneyuki, Masaki Sano
  • Organizer: 日本生物物理学会
  • Year and Date: 2010-09-22
• [Presentation] Information-heat engine as a model system of molecular motors (2010)
  • Author(s): Shoichi Tayabe
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-22
• [Presentation] Experimental demonstration of information-energy conversion (2010)
  • Author(s): Shoichi Toyabe, Takahiro Sagawa, Masahito Ueda, Eiro Muneyuki, Masaki Sano
  • Organizer: StatPhys 24
  • Place of Presentation: Cairns Convention Center (Austoraria)
  • Year and Date: 2010-07-23
• [Presentation] Experimental demonstration of information-energy conversion (2010)
  • Author(s): Shoichi Toyabe
  • Organizer: Statphys 24
  • Place of Presentation: ケアンズ(オーストラリア)
  • Year and Date: 2010-07-23
• [Presentation] Single-molecule nonequilibrium energetics of a molecular-motor F_1-ATPase (2010)
  • Author(s): Shoichi Toyabe
  • Organizer: Mini-synposium on Liquids
  • Place of Presentation: 九州大学
  • Year and Date: 2010-06-26
• [Presentation] Single-molecule nonequilibrium energetics of a molecular-motor F1-ATPase (2010)
  • Author(s): Shoichi Toyabe
  • Organizer: Mini-symposium on Liquids
  • Place of Presentation: 九州大学
  • Year and Date: 2010-06-26
• [Presentation] Nonequilibrium energetics of a single F1-ATPase molecule (2010)
  • Author(s): Shoichi Toyabe
  • Organizer: アメリカ生物物理学会
  • Place of Presentation: Moscone center(サンフランシスコ)
  • Year and Date: 2010-02-21
• [Presentation] Nonequilibrium energetics of a single F_1-ATPase molecule (2010)
  • Author(s): Shoichi Toyabe, Takahiro Watanabe-Nakayama, Tetsuaki Okamoto, Seishi Kudo, Eiro Muneyuki
  • Organizer: Biophysical society, Annual meeting
  • Place of Presentation: SanFrancisco (USA)
• [Presentation] Mechanochemical coupling and energetics of F_1-ATPase (2009)
  • Author(s): Shoichi Toyabe
  • Organizer: International Workshop on Dynamic Cross-effect in Softy Condensed matter
  • Place of Presentation: 東京
  • Year and Date: 2009-11-05
• [Presentation] Mechanochemical coupling and energetics of F1-ATPase (2009)
  • Author(s): Shoichi Toyabe
  • Organizer: International workshop on dynamic cross-effect in softly condensed matter
  • Place of Presentation: 国立情報学研究所
  • Year and Date: 2009-11-05
• [Presentation] F_1-ATPaseのストールトルク (2009)
  • Author(s): 鳥谷部祥一, 中山隆宏, 岡本哲明, 工藤成史, 宗行英朗
  • Organizer: 日本生物物理学会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] Stall torque of F_1-ATPase (2009)
  • Author(s): 鳥谷部祥一
  • Organizer: 日本生物物理学会第47回大会
  • Place of Presentation: アスティとくしま(徳島)
  • Year and Date: 2009-10-31
• [Presentation] F_1-ATPaseの1分子非平衡熱力学 (2009)
  • Author(s): 鳥谷部祥一
  • Organizer: FoF1勉強会
  • Place of Presentation: 横浜市立大学
  • Year and Date: 2009-10-20
• [Presentation] F_1-ATPaseの1分子非平衡熱力学 (2009)
  • Author(s): 鳥谷部祥一
  • Organizer: F_0F_1勉強会
  • Place of Presentation: 横浜市立大学
  • Year and Date: 2009-10-20
• [Presentation] 回転分子モーターF_1-ATPaseのストールトルク (2009)
  • Author(s): 鳥谷部祥一
  • Organizer: 日本物理学会2009年度秋季大会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-27
• [Presentation] 回転分子モーターF_1-ATPaseのストールトルク (2009)
  • Author(s): 鳥谷部祥一, 中山隆宏, 岡本哲明, 工藤成史, 宗行英朗
  • Organizer: 日本生物物理学会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-21
• [Presentation] F_1-ATPaseのストールトルク (2009)
  • Author(s): 鳥谷部祥一
  • Organizer: 革新的ナノサイエンス第4回班会議
  • Place of Presentation: 万国津梁館(沖縄)
  • Year and Date: 2009-06-17