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Crosstalk between the local temperature change inside single living cells and the cellular functions

Research Project

Project/Area Number 21740316
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biophysics/Chemical physics
Research InstitutionWaseda University

Principal Investigator

SUZUKI Madoka  Waseda University, 総合研究機構, 講師 (40350475)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords生物物理 / 単一細胞 / 蛍光イメージング / 温度 / 熱 / 顕微鏡
Research Abstract

The purpose of this study is to clarify the relationship between the temperature and the functions of the brain and the heart in which the [Ca^<2+>] strongly coupled with their function in the microscopic view point. We first focused on clarifying the thermal properties of single living cells. Then the effect of the local temperature gradient was studied with the use of dividing single HeLa cells. Furthermore, the response of the single motile cells to the local temperature gradient and the heat pulse was analyzed. In the meantime, the research was expanded to the studies to elucidate the mechanism of the oscillatory movement of the cardiac cell.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (39 results)

All 2011 2010 2009

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (36 results)

  • [Journal Article] Molecular motors as an auto-oscillator.2010

    • Author(s)
      Ishiwata, S., Shimamoto, Y., Suzuki, M.
    • Journal Title

      HFSP J. 4(3)

      Pages: 100-104

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular motors as an auto-oscillator.2010

    • Author(s)
      Ishiwata, S., Shimamoto, Y., Suzuki, M. (Equal contribution)
    • Journal Title

      HFSP J.

      Volume: 4 Pages: 100-104

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular motors as an auto-oscillator.2010

    • Author(s)
      S.Ishiwata, Y.Shimamoto, M.Suzuki.
    • Journal Title

      HFSP J. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Microscopic heat pulse induced calcium dynamics in a migratory cell.2011

    • Author(s)
      Itoh, H., Suzuki, M., Oyama, K., Motoyoshi, M., Ishiwata, S.
    • Organizer
      Biophysical Society the 55th Annual Meeting
    • Place of Presentation
      Baltimore, Maryland, USA
    • Year and Date
      2011-03-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Rearrangement of mitotic spindle in HeLa cell under steep temperature gradient.2011

    • Author(s)
      Motoyoshi, M., Suzuki, M., Oyama, K., Itabashi, T., Ishiwata, S.
    • Organizer
      Biophysical Society the 55th Annual Meeting
    • Place of Presentation
      Baltimore, Maryland, USA
    • Year and Date
      2011-03-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] レーザー誘起気泡で生じる温度変化の時空間分布2011

    • Author(s)
      鈴木団
    • Organizer
      第3回FBT(生物学・化学・情報科学融合のための戦略的研究基盤形成支援事業)シンポジウム
    • Place of Presentation
      早稲田大学、東京
    • Year and Date
      2011-01-22
    • Related Report
      2010 Final Research Report
  • [Presentation] レーザー誘起気泡で生じる温度変化の時空間分布2011

    • Author(s)
      鈴木団
    • Organizer
      第3回 FBT(生物学・化学・情報科学融合のための戦略的研究基盤形成支援事業)シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2011-01-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Microscopic heat pulse induced calcium dynamics in a migratory cell.2011

    • Author(s)
      Itoh, H., Suzuki, M., Oyama, K., Motoyoshi, M., Ishiwata, S.
    • Organizer
      米国生物物理学会第55回年会(Biophysical Society the 55th Annual Meeting)
    • Place of Presentation
      Baltimore, Maryland, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Rearrangement of mitotic spindle in HeLa cell under steep temperature gradient.2011

    • Author(s)
      Motoyoshi, M., Suzuki, M., Oyama, K., Itabashi, T., Ishiwata, S.
    • Organizer
      米国生物物理学会第55回年会(Biophysical Society the 55th Annual Meeting)
    • Place of Presentation
      Baltimore, Maryland, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] 生体内の微小な温度変化の検出を目的とした蛍光ナノ温度計の創製2010

    • Author(s)
      高林昌生、新井敏、大山廣太郎、鈴木団、石渡信一、武岡真司
    • Organizer
      第32回日本バイオマテリアル学会大会
    • Place of Presentation
      広島
    • Year and Date
      2010-11-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Rotation of mitotic spindle in HeLa cell under steep temperature gradient.2010

    • Author(s)
      Motoyoshi, M., Suzui, M., Oyama, K., Itabashi, T., Ishiwata, S.
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ca2+ response to microscopic heat pulses in a migrant cell.2010

    • Author(s)
      Itoh, H., Suzuki, M., …, Ishiwata, S
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Microscopic measurement of periodic rigor cross-bridge formation and their unbinding force in rabbit skeletal myofibril.2010

    • Author(s)
      Suzuki, M., Ishiwata, S.
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] High-speed imaging of heat transfer in single living cells.2010

    • Author(s)
      Oyama, K., Suzuki, M., …, Ishiwata, S.
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mechanobiology in Muscle Contraction2010

    • Author(s)
      Ishiwata, S., Shimamoto, Y., Ohtaki, M., Sato, K., Suzuki, M.
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      仙台(招待講演)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Investigation of the role of micro-level physical parameters in biological systems.2010

    • Author(s)
      Suzuki, M.
    • Organizer
      The 1st Anniversary Symposium of Waseda Bioscience Research Institute in Singapore (WABIOS)
    • Place of Presentation
      Singapore
    • Year and Date
      2010-09-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Fluorescence imaging of a heat transfer in single HeLa cells.2010

    • Author(s)
      Oyama, K., Suzuki, M., Tseeb, V., Seto, Y., Motoyoshi, M., Itoh, H., Iwai, K., Ishiwata, S.
    • Organizer
      The 1st Anniversary Symposium of Waseda Bioscience Research Institute in Singapore (WABIOS)
    • Place of Presentation
      Singapore
    • Year and Date
      2010-09-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Investigation of the role of micro-level physical parameters in biological systems2010

    • Author(s)
      Suzuki, M.
    • Organizer
      The 1st Anniversary Symposium of Waseda Bioscience Research In stitute in Singapore (WABIOS)
    • Place of Presentation
      Biopolis, Singapore
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Fluorescence imaging of a heat transfer in single HeLa cells.2010

    • Author(s)
      Oyama, K., Suzuki, M., …, Ishiwata, S.
    • Organizer
      The 1st Anniversary Symposium of Waseda Bioscience Research In stitute in Singapore (WABIOS)
    • Place of Presentation
      Biopolis, Singapore
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of microscopic imaging techniques and study into cell structure, physical properties and function.2010

    • Author(s)
      Suzuki, M., Oyama, K., Seto, Y., Ishiwata, S.
    • Organizer
      第2回FBT(生物学・化学・情報科学融合のための戦略的研究基盤形成支援事業)、PBL(大学院教育改革支援プログラム)ジョイントシンポジウム
    • Place of Presentation
      早稲田大学、東京
    • Year and Date
      2010-01-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 顕微画像化法の開発と細胞構造・物性・機能の研究2010

    • Author(s)
      鈴木団
    • Organizer
      第2回FBT\TSC-PBLジョイントシンポジウム
    • Place of Presentation
      早稲田大学,東京
    • Year and Date
      2010-01-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Microscopic measurement of periodic rigor cross-bridge formation and their unbinding force in rabbit skeletal myofibril.2010

    • Author(s)
      Suzuki, M., Ishiwata, S.
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Sendai
    • Related Report
      2010 Final Research Report
  • [Presentation] High-speed imaging of heat transfer in single living cells.2010

    • Author(s)
      Oyama, K., Suzuki, M., Tseeb, V., Seto, K., Motoyoshi, M., Itoh, H., Iwai, K., Ishiwata, S.
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Sendai
    • Related Report
      2010 Final Research Report
  • [Presentation] Ca^<2+> response to microscopic heat pulses in a migrant cell.2010

    • Author(s)
      Itoh, H., Suzuki, M., Oyama, K., Motoyoshi, M., Seto, Y., Ishiwata, S.
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Sendai
    • Related Report
      2010 Final Research Report
  • [Presentation] Rotation of mitotic spindle in HeLa cell under steep temperature gradient.2010

    • Author(s)
      Motoyoshi, M., Suzui, M., Oyama, K., Itabashi, T., Ishiwata, S.
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Sendai
    • Related Report
      2010 Final Research Report
  • [Presentation] Mechanobiology in Muscle Contraction.2010

    • Author(s)
      Ishiwata, S., Shimamoto, Y., Ohtaki, M., Sato, K., Suzuki, M.
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Sendai
    • Related Report
      2010 Final Research Report
  • [Presentation] Microscopic measurement of periodic cross-bridge formation in skeletal myofibril.2010

    • Author(s)
      Suzuki, M., Ishiwata, S.
    • Organizer
      米国生物物理学会第54回年会Biophysical Society 54th Annual Meeting, San Francisco, CA
    • Place of Presentation
      Ishiwata
    • Related Report
      2010 Final Research Report
  • [Presentation] Self-organization in Biomotile Systems-Molecular motors, Auto-oscillation (SPOC) in muscle and Meiotic spindle-2010

    • Author(s)
      Y.Oguchi, S.V.Mikhailenko, M.Suzuki, K.Sato, M.Ohtaki, Y.Shimamoto, K.Suzuki, J.Takagi, T.Itabashi
    • Organizer
      1st POSTECH Workshop on Physics of Self-Organization in Bio/Nano-Systems, Asia Pacific Center for Theoretical Physics (APCTP), Pohang, S.Korea
    • Related Report
      2010 Final Research Report
  • [Presentation] 拍動に伴う心筋細胞の熱産生イメージング2010

    • Author(s)
      瀬戸祐介、大山廣太郎、新谷正嶺、福田紀男、鈴木団、石渡信一
    • Organizer
      2010年生体運動研究合同班会議プログラム
    • Place of Presentation
      中央大学、東京
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] MICROSCOPIC MEASUREMENT OF PERIODIC CROSS-BRIDGE FORMATION IN SKELETAL MYOFIBRIL2010

    • Author(s)
      M.Suzuki, S.Ishiwata
    • Organizer
      Biophysical Society 54th Annual Meeting
    • Place of Presentation
      San Francisco, CA
    • Related Report
      2009 Annual Research Report
  • [Presentation] Self-organization in Bianotile Systems-Molecular motors, Auto-oscillation (SPOC) in muscle and Meiotic spindle-2010

    • Author(s)
      S.Ishiwata, et al.
    • Organizer
      1st POSTECH Workshop on Physics of Self-Organization in Bio/Nano-Systems
    • Place of Presentation
      Pohang, S. Korea
    • Related Report
      2009 Annual Research Report
  • [Presentation] Chemo-mechanical Feedback Loop in Biomotile Systems, International Conference on Physics Biology Interface2009

    • Author(s)
      S.Ishiwata, Y.Oguchi, S.V.Mikhailenko, M.Suzuki, K.Sato, M.Ohtaki, Y.Shimamoto, K.Suzuki, J.Takagi, T.Itabashi
    • Organizer
      Saha Institute of Nuclear Physics
    • Place of Presentation
      Kolkata, India
    • Related Report
      2010 Final Research Report
  • [Presentation] Direct observation of interaction between single actin filament and myosin in skeletal myofibril.2009

    • Author(s)
      Suzuki, M., Ishiwata, S.
    • Organizer
      The 3rd Mechanobiology Workshop, National University of Singapore
    • Place of Presentation
      Singapore
    • Related Report
      2010 Final Research Report
  • [Presentation] 分裂期のHeLa細胞への局所温度勾配の効果Effect of local temperature gradient on HeLa cells in mitotic phase.2009

    • Author(s)
      Motoyoshi, M., Suzuki, M., Oyama, K., Itabashi, T., Ishiwata, S.
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      徳島
    • Related Report
      2010 Final Research Report
  • [Presentation] 肥満細胞の脱顆粒メカニズム:アクチン骨格再構成での活性化タンパク質Cの役割Mechanism of mast cell degranulation : Roles of activated protein C in the rearrangement of actin cytoskeleton.2009

    • Author(s)
      Ono, F., Suzuki, M., Oyama, K., Strukova, S., Ishiwata, S.
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      徳島
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 細胞内の局所的な温度変化の測定とその解析Measurement and analysis of local temperature change inside living cells.2009

    • Author(s)
      Oyama, K., Suzuki, M., Tseeb, V., Ono, F., Seto, Y., Motoyoshi, M., Iwai, K., Ishiwata, S.
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      徳島
    • Related Report
      2010 Final Research Report
  • [Presentation] 分裂期のHeLa細胞への局所温度勾配の効果Effect of Iocal temperature gradient on HeLa cells in mitotic phase.2009

    • Author(s)
      M.Motoyoshi, M.Suzuki, K.Oyama, T.Itabashi, S.Ishiwata
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      徳島
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細胞内の局所的な温度変化の測定とその解析Measurement and analysis of Iocal temperature change inside living cells.2009

    • Author(s)
      K.Oyama, et al.
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      徳島
    • Related Report
      2009 Annual Research Report
  • [Presentation] Direct observation of interaction between single actin filament and myosin in skeletal myofibril.2009

    • Author(s)
      Madoka Suzuki, Shin'ichi Ichiwata
    • Organizer
      The 3rd Mechanobiology Workshop
    • Place of Presentation
      NUS, Singapore
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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