| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Research Abstract |
Novel fluorescent probes for detecting metal ions working in capillary electrophoresi-laser-induced fluorescence detection (CE-LIF) and polyacrylamide gel electrophoresis (PAGE) have been developed. The detection limits for several transition metal ions of tens pM were successfully obtained. In CE-LIF, the addition of cationic polymer provided high resolution among metal-probe complexes with new separation principle. The new separation mode was named as "Ion association complex separation mode." In PAGE, Fe and Cu ions which existed in the gel fractions of transferrin and ceruloplasmin were successfully determined.
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