Development of fluorescent reagents for the real-time imaging of intracellular redox state

• Project/Area Number: 21750077
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Saga University (2011); Kyushu University (2009-2010)
• Principal Investigator: SOH Nobuaki 佐賀大学, 農学部, 准教授 (90336008)
• Research Collaborator: IMATO Toshihiko 九州大学, 大学院・工学研究院, 教授 (50117066)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: レドックス / 蛍光 / 分析科学 / 蛍光試薬

Research Abstract

Novel fluorescent reagents were developed for monitoring redox state. The fluorescent reagents(Cy-NQ, PBI-AQ) consist of fluorophore unit(cyanine(Cy), perylene bisimide(PBI)) and redox-responsive unit(naphthoquinone(NQ), anthraquinone(AQ). Fluorescent responses of the fluorescent reagents were observed based on the redox reactions.

Research Products

• [Journal Article] Perylenebisimide as a versatile fluorescent tool for environmental and biological analysis : A review (2011)
  • Author(s): Nobuaki Soh, Toshihisa Ueda
  • Journal Title: Talanta Volume: 85 Pages: 1233-1237
  • Peer Reviewed
• [Journal Article] ペリレンビスイミドを用いた分析法 (2011)
  • Author(s): 宗伸明
  • Journal Title: ぶんせき Volume: 10 Pages: 619-620
  • NAID: 10029881975
  • Peer Reviewed
• [Presentation] Observation of flow behavior of fluorescence probe in microchannel by using confocal microscopy (2011)
  • Author(s): Tomoharu Maki, Ryoichi Ishimatsu, Nobuaki Soh, Koji Nakano, Toshihiko Imato
  • Organizer: Mini-Symposium on Flow Injection Analysis with Professor Gary D. Christian
  • Place of Presentation: Aichi, Japan
  • Year and Date: 2011-12-02
• [Presentation] Observation of Flow Behavior of Fluorescence Probe in Microchannel by Using Confocal Microscopy (2011)
  • Author(s): 牧知治・宗伸明, ら
  • Organizer: 第49回フローインジェクション分析講演会
  • Place of Presentation: 愛知県豊田市
  • Year and Date: 2011-12-02
• [Presentation] バイオ分析のためのケミカルツール (2011)
  • Author(s): 宗伸明
  • Organizer: 2011年日本化学会西日本大会
  • Place of Presentation: 徳島県徳島市
  • Year and Date: 2011-11-13
• [Presentation] 細胞内レドックス環境のイメージング計測を指向した酸化還元型蛍光センサーの開発 (2010)
  • Author(s): 亀田真吾、久保雄也、宗伸明、中野幸二、今任稔彦
  • Organizer: 2010年日本化学会西日本大会
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2010-11-07
• [Presentation] "Fluorescent chemical tools for bioanalysis", Kyushu University Global COE Program" Science for Future Molecular Systems" (2010)
  • Author(s): Nobuaki Soh
  • Organizer: The 13th International Workshop
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2010-10-29
• [Presentation] Fluorescent Chemical Tools for Bioanalysis (2010)
  • Author(s): Nobuaki SOH
  • Organizer: Kyushu University Global COE Program "Science for Future Molecular Systems", The 13th International Workshop
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2010-10-29
• [Presentation] 細胞内レドックスの可視化を指向した蛍光プローブの開発 (2010)
  • Author(s): 亀田真吾、久保雄也、宗伸明、中野幸二、今任稔彦
  • Organizer: 第28回九州分析化学若手の会夏季セミナー
  • Place of Presentation: 長崎県南島原市
  • Year and Date: 2010-07-30
• [Presentation] 細胞内レドックスの可視化を指向した蛍光プローブの開発 (2010)
  • Author(s): 亀田真吾、久保雄也、宗伸明、中野幸二、今任稔彦
  • Organizer: 第47回化学関連支部合同九州大会
  • Place of Presentation: 福岡県北九州市
  • Year and Date: 2010-07-10
• [Presentation] Development of novel fluorescent probes for monitoring biomolecules (2009)
  • Author(s): Nobuaki Soh
  • Organizer: Research Topics in Biomedical Engineering, ETH Zurich(Fall Semester 2009)
  • Place of Presentation: Zurich, Switzerland
  • Year and Date: 2009-11-09
• [Presentation] 細胞機能解析を指向した小分子蛍光プローブ群の創製と応用 (2009)
  • Author(s): 宗伸明
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2009-09-25
• [Presentation] Development of Novel Fluorescent Probes for Monitoring Biomolecules (2009)
  • Author(s): Nobuaki SOH
  • Organizer: Research Topics in Biomedical Engineering, ETH Zurich
  • Place of Presentation: Zurich, Switzerland
  • Year and Date: 2009-09-11
• [Presentation] 活性酸素種センシング試薬の開発と応用 (2009)
  • Author(s): 宗伸明、牧知治、有吉知幸、中野幸二、今任稔彦
  • Organizer: 東京コンファレンス2009
  • Place of Presentation: 千葉県千葉市
  • Year and Date: 2009-09-04
• [Presentation] 細胞内を探る分子ツール (2009)
  • Author(s): 宗伸明
  • Organizer: 第22回九州分析化学若手の会春の講演会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2009-05-23