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Gene sensors based on "signal-on"-typed electrochemical sensingmethods

Research Project

Project/Area Number 21750085
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

AOKI Hiroshi  National Institute of Advanced Industrial Science and Technology, 環境管理技術研究部門, 主任研究員 (00392580)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords化学センサー / 電気化学 / バイオセンサ / DNA / 遺伝子検出 / 超分子電気化学 / 遺伝子プローブ / ラベル化不要 / シグナル・オン
Research Abstract

To promote high-sensitive gene detection methods with simplicity and rapidity, a novel "signal-on"-typed gene probe was developed based on dissociation of a redox complex triggered by DNA recognition. The probe enabled simple DNA detection for non-labeled DNAs with 5-fold increase in observed redox current. Moreover, gene sensing devices were developed to simultaneously detect multiple DNAs with simplicity, rapidity, and sequence-specificity.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (48 results)

All 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (29 results) Remarks (5 results) Patent(Industrial Property Rights) (9 results) (of which Overseas: 1 results)

  • [Journal Article] Electrochemical Sensor Array Chips for Multiple Gene Detection2010

    • Author(s)
      H Aoki, A.Kitajima, H.Tao
    • Journal Title

      Sensors & Materials 22

      Pages: 327-336

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Label-free and "signal-on" DNA detection using a probe DNA terminated with ferrocene and β-cyclodextrin2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Journal Title

      Supramolecular Chemistry 22

      Pages: 455-460

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Gene Sensor Arrays Prepared Using Non-contact Nanoliter Array Spotting of Gene Probes2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Journal Title

      Analytical Sciences 26

      Pages: 367-370

    • NAID

      10026782541

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Sensor Array Chips for Multiple Gene Detection2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Journal Title

      Sensors & Materials

      Volume: 22 Pages: 327-336

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Label-free and "signal-on" DNA detection using a probe DNA terminated with ferrocene and ss-cyclodextrin2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Journal Title

      Supramolecular Chemistry (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Label-free, reagent-free, and "signal-on" DNA detection based on supramolecular electrochemistry2011

    • Author(s)
      H.Aoki
    • Organizer
      ICBS2010(国際学会、口頭発表)
    • Place of Presentation
      Tsukuba
    • Year and Date
      2011-03-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Label-free, reagent-free, and "signal-on" DNA detection based on supramolecular electrochemistry2011

    • Author(s)
      H.Aoki
    • Organizer
      International Conference on Biomaterials Science 2011 (ICBS 2011)
    • Place of Presentation
      つくば
    • Year and Date
      2011-03-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 簡便・迅速な遺伝子検出法の開発とデバイス化2011

    • Author(s)
      青木寛、北島明子、鳥村政基
    • Organizer
      産総研・産技連LS-BT合同研究発表会(受賞、国内学会、口頭発表)
    • Place of Presentation
      つくば
    • Year and Date
      2011-02-01
    • Related Report
      2010 Final Research Report
  • [Presentation] 簡便・迅速な遺伝子検出法の開発とデバイス化2011

    • Author(s)
      青木寛、北島明子、鳥村政基
    • Organizer
      産総研・産技連LS-BT合同研究発表会
    • Place of Presentation
      つくば(受賞)
    • Year and Date
      2011-02-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] 電気化学的手法によるDNA検出とDNAエレクトロニクスへの展望2011

    • Author(s)
      青木寛
    • Organizer
      第8回バイオナノエレクトロニクス学術講演会(招待講演、国内学会、口頭発表)
    • Place of Presentation
      秦野
    • Year and Date
      2011-01-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 電気化学的手法によるDNA検出とDNAエレクトロニクスへの展望2011

    • Author(s)
      青木寛
    • Organizer
      第8回バイオナノエレクトロニクス学術講演会
    • Place of Presentation
      秦野(招待講演)
    • Year and Date
      2011-01-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Label-free, reagent-free, and "signal-on" DNA detection based on supramolecular electrochemistry2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Organizer
      Pacifichem2010(受賞、国際学会、口頭発表)
    • Place of Presentation
      Honolulu, HI, USA
    • Year and Date
      2010-12-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Label-free, reagent-free, and "signal-on" DNA detection based on supramolecular electrochemistry2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Organizer
      Pacifichem 2010(2010環太平洋国際化学会議)
    • Place of Presentation
      Honolulu, USA(受賞)
    • Year and Date
      2010-12-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Label-free and reagent-free DNA detection based on supramolecular electrochemistry2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Organizer
      Nanojasp2010(招待講演、国際学会、口頭発表)
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-11-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Label-free and reagent-free DNA detection based on supramolecular electrochemistry2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Organizer
      Nanojasp 2010
    • Place of Presentation
      Barcelona(招待講演)
    • Year and Date
      2010-11-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高速スクリーニング用二次元ピッチ可変型アレイスポッタ2010

    • Author(s)
      青木寛、鳥村政基
    • Organizer
      第8回環境エネルギー分野交流会(E&Eフォーラム)(招待講演、国内学会、口頭発表)
    • Place of Presentation
      つくば
    • Year and Date
      2010-10-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 高速スクリーニング用二次元ピッチ可変型アレイスポッタ2010

    • Author(s)
      青木寛、鳥村政基
    • Organizer
      第8回環境エネルギー分野交流会(E&Eフォーラム)
    • Place of Presentation
      つくば(招待講演)
    • Year and Date
      2010-10-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Label-free and reagent-free DNA detection based on supramolecular electrochemistry2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Organizer
      The 61st Annual Meeting of the International Society of Electrochemistry(国際学会、口頭発表)
    • Place of Presentation
      Nice, France
    • Year and Date
      2010-09-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Label-free and reagent-free DNA detection based on supramolecular electrochemistry2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Organizer
      61^<st> Annual Meeting of the International Society of Electrochemistry (ISE)
    • Place of Presentation
      Nice, France
    • Year and Date
      2010-09-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] フェロセンおよびシクロデキストリン末端修飾プローブによる非標識DNAの超分子電気化学検出2010

    • Author(s)
      青木寛、北島明子、鳥村政基、田尾博明
    • Organizer
      日本分析化学会第59年会(国内学会、口頭発表)
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-15
    • Related Report
      2010 Final Research Report
  • [Presentation] フェロセンおよびシクロデキストリン末端修飾プローブによる非標識DNAの超分子電気化学検出2010

    • Author(s)
      青木寛、北島明子、鳥村政基、田尾博明
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 固液界面での分子ダイナミクスに基づく電気化学的な自己報告型遺伝子検出デバイス2010

    • Author(s)
      青木寛
    • Organizer
      科研費・新学術領域 第五回領域会議、国立循環器病研究センター(国内学会、口頭発表)
    • Place of Presentation
      吹田
    • Year and Date
      2010-07-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Label-Free and Reagent-Free DNA Detection Based on Supramolecular Electrochemistry2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Organizer
      Biosensors 2010(国際学会、ポスター発表)
    • Place of Presentation
      Glasgow, UK
    • Year and Date
      2010-05-26
    • Related Report
      2010 Final Research Report
  • [Presentation] Label-free and reagent-free DNA detection based on supramolecular electrochemistry2010

    • Author(s)
      H.Aoki, A.Kitajima, H.Tao
    • Organizer
      Biosensors 2010
    • Place of Presentation
      Glasgow, UK
    • Year and Date
      2010-05-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] ハイブリッド形成により構造変化するフェロセン標識プローブによる遺伝子検出とシグナル増幅2009

    • Author(s)
      青木寛、北島明子、田尾博明
    • Organizer
      第19回日本MRS学術シンポジウム(国内学会、口頭発表)
    • Place of Presentation
      波止場会館、横浜
    • Year and Date
      2009-12-07
    • Related Report
      2010 Final Research Report
  • [Presentation] ハイブリッド形成により構造変化するフェロセン標識プローブによる遺伝子検出とシグナル増幅2009

    • Author(s)
      青木寛、北島明子、田尾博明
    • Organizer
      第19回日本MRS学術シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] signal Enhancement for Gene Detection Using Ferrocene Terminated Probes Based on Hybridization-Amenable Conformation Flexibility2009

    • Author(s)
      H.Aoki, H.Tao
    • Organizer
      Bio-Sensing Technology Conference 2009(国際学会、ポスター発表)
    • Place of Presentation
      Bristol, UK
    • Year and Date
      2009-11-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Signal Enhancement for Gene Detection Using Ferrocene-Terminated Probes Based on Hybridization-Amenable Conformation Flexibility2009

    • Author(s)
      H.Aoki, H.Tao
    • Organizer
      Bio-Sensing Technology Conference 2009
    • Place of Presentation
      ブリストル(イギリス)
    • Year and Date
      2009-11-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] 電気化学的遺伝子検出法の開発と迅速遺伝子診断技術の開発2009

    • Author(s)
      青木寛
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学、北海道(国内発表、口頭発表(招待講演))
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 電気化学的遺伝子検出法の開発と迅速遺伝子診断技術の開発2009

    • Author(s)
      青木寛
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 電気化学的遺伝子検出法の開発と迅速診断技術への展開、2009年電気化学秋季大会2009

    • Author(s)
      青木寛、北島明子、田尾博明
    • Organizer
      東京農工大学小金井キャンパス(国内学会、口頭発表(招待講演))
    • Place of Presentation
      東京
    • Year and Date
      2009-09-10
    • Related Report
      2010 Final Research Report
  • [Presentation] 電気化学的遺伝子検出法の開発と迅速診断技術への展開2009

    • Author(s)
      青木寛、北島明子、田尾博明
    • Organizer
      2009年電気化学秋季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] 超分子電気化学に基づく遺伝子検出法2009

    • Author(s)
      青木寛、北島明子、田尾博明
    • Organizer
      東京コンファレンス2009(国内学会、ポスター発表)
    • Place of Presentation
      幕張メッセ、東京
    • Year and Date
      2009-09-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 超分子電気化学に基づく遺伝子検出法2009

    • Author(s)
      青木寛、北島明子、田尾博明
    • Organizer
      東京コンファレンス2009
    • Place of Presentation
      東京
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Remarks] 受賞(計4件)

    • Related Report
      2010 Final Research Report
  • [Remarks] 優秀ポスター賞、青木寛、北島明子、鳥村政基:簡便・迅速な遺伝子検出法の開発とデバイス化、産総研・産技連LS-BT合同研究発表会、2010年.

    • Related Report
      2010 Final Research Report
  • [Remarks] ハイライト講演、H.Aoki, A.Kitajima, H.Tao, Label-free, reagent-free, and "signal-on" DNA detection based on supramolecular electrochemistry, Pacifichem2010、2010年.

    • Related Report
      2010 Final Research Report
  • [Remarks] 奨励賞、青木寛、電気化学的遺伝子検出法の開発と迅速遺伝子診断技術の開発、日本分析化学会、2009年.

    • Related Report
      2010 Final Research Report
  • [Remarks] 優秀ポスター賞、青木寛、北島明子、田尾博明、超分子電気化学に基づく遺伝子検出法、東京コンファレンス2009年.

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 可変ピッチアレイスポッタ2011

    • Inventor(s)
      青木寛、鳥村政基、田尾博明、池田隆至
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Acquisition Date
      2011-02-25
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 可変ピッチアレイスポッタ2011

    • Inventor(s)
      青木寛、鳥村政基、田尾博明、池田隆至
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Acquisition Date
      2011-02-25
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 分注装置2010

    • Inventor(s)
      青木寛、鳥村政基、田尾博明
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Number
      2010-292724
    • Filing Date
      2010-12-28
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Patent(Industrial Property Rights)] 可変ピッチアレイスポッタ2010

    • Inventor(s)
      青木寛、鳥村政基、田尾博明、池田隆至
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Number
      2010-267350
    • Filing Date
      2010-11-30
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Patent(Industrial Property Rights)] Electrochemical molecular recognition probes2010

    • Inventor(s)
      H.Aoki, A.Kitajima, H.Tao
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Filing Date
      2010-08-31
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 電気化学分子認識プローブ及びそれを用いた分子認識センサ並びにそれらを用いた電気化学的検出方法2010

    • Inventor(s)
      青木寛、北島明子、田尾博明
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Number
      2010-193207
    • Filing Date
      2010-08-31
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Patent(Industrial Property Rights)] Electrochemical molecular recognition probes2010

    • Inventor(s)
      青木寛、北島明子、田尾博明
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Filing Date
      2010-08-31
    • Related Report
      2010 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 電気化学分子認識プローブ2009

    • Inventor(s)
      青木寛、北島明子、田尾博明
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Number
      2009-242921
    • Filing Date
      2009-10-22
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 電気化学分子認識プローブ2009

    • Inventor(s)
      青木寛、北島明子、田尾博明
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Number
      2009-208400
    • Filing Date
      2009-09-09
    • Related Report
      2010 Final Research Report 2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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