Identification of cellulose-binding peptides and its application for cellulase reaction

Research Project

Project/Area Number	21750112
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Polymer chemistry
Research Institution	Kyushu University
Principal Investigator	MATSUNO Hisao Kyushu University, 大学院・工学研究院, 准教授 (50376696)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords	生体関連高分子 / ペプチド / セルロース / 表面吸着 / 高分子表面
Research Abstract	Phage libraries displaying random and linear hepta/dedecapeptides were applied to crystalline cellulose. Binding affinities of obtained phage clones were quantitatively analyzed by enzymelinked immunosorbent assays (ELISAs). Clones were obtained with greater apparent affinity constants than those of the library, suggesting the isolation of cellulose-binding peptides with characteristic sequences. Furthermore, synthetic peptides with identify sequences showed the affinity constant, which was determined by high performance liquid chromatography, 10^5-10^6 mol/L corresponding to that of natural protein from cellulose binding domain.