| Project/Area Number |
21750162
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKEGAWA Yasuhiro Hokkaido University, 大学院・先端生命科学研究院, 特任助教 (60515071)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | バイオテクノロジー / コンドロイチン硫酸(CS) / ヘパラン硫酸(HS) / ヒアルロン酸(HA) / グリコサミノグリカン(GAG) / 両性イオンクロマトグラフィー / グライコブロッティング / 培養細胞 / 再生医療 / コンドロイチン硫酸 / 質量分析 / MS^n / 構造-機能相関 / 両性イオン型クロマトグラフィー / グリコサミノグリカン / LC / MS |
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| Research Abstract |
We established simple isolation and simultaneous analytical techniques for disaccharides derived from all major glycosaminoglycans (chondroitin sulfate, heparan sulfate, hyaluronic acid), which have been difficult thus far, by using combination glycoblotting techqnique with zwitterionic interaction chromatography. The established technique was applied for various cultured cells, and we demonstrated the usefulness of this technique for the classification of cell type. We also explored the tissue-engineered cultured cornea sample, which are used as cornea reconstruction with the cornea epithelium and oral epithelium. As a result, we showed a novel finding about cornea regenerative medicine.
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