• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of time-resolved circular dichroism measurement and its application to the detection of conformational dynamics in DNA-ligand complex

Research Project

Project/Area Number 21750164
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Chemistry related to living body
Research InstitutionTohoku University

Principal Investigator

ARAKI Yasuyuki  東北大学, 多元物質科学研究所, 助教 (80361179)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords生体機能関連化学 / 円二色性 / DNA / 色素
Research Abstract

We aimed to develop the time-resolved circular dichroism measurement for the purpose of the detection of the dynamic behavior of this DNA-drug interactions, focusing on an entire chirality occurring as a result of DNA-drug interaction. We have successfully developed it and detected the dynamic behavior of DNA-drug interactions caused by the laser-induced conformational change of DNA-drug complexes.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (24 results)

All 2011 2010 2009

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (14 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Fullerene- and Pyromellitdiimide-Appended Tripodal Ligands Embedded in Light-Harvesting Porphyrin Macrorings2011

    • Author(s)
      Kuramochi, Y., Araki, Y, 他4名
    • Journal Title

      Inorg. Chem.

      Volume: 50 Pages: 10249-10258

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple photosynthetic reaction centres composed of supramolecular assemblies of zinc porphyrin dendrimers with a fullerene acceptor2011

    • Author(s)
      Fukuzumi, S., Saito, K., Ohkubo, K., Kashiwagi, Y., Araki, Y., 他6名
    • Journal Title

      Chem. Commun.

      Volume: 47 Pages: 7980-7982

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fullerene- and Pyromellitdiimide-Appended Tripodal Ligands Embedded in Light-Harvesting Porphyrin Macrorings2011

    • Author(s)
      Kuramochi, Y.; Satake, A.; Sandanayaka, A.S.D.; Araki, Y.; Ito, O.; Kobuke, Y.
    • Journal Title

      Inorg.Chem.

      Volume: 50 Pages: 10249-10258

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple photosynthetic reaction centres composed of supramolecular assemblies of zinc porphyrin dendrimers with a fullerene acceptor2011

    • Author(s)
      Fukuzumi, S.; Saito, K.; Ohkubo, K.; Khoury, T.; Kashiwagi, Y.; Absalom, M.A.; Gadde, S.; D'Souza, F.; Araki, Y.; Ito, O.; Crossley, M.J.
    • Journal Title

      Chem.Commun.

      Volume: 47 Pages: 7980-7982

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Axle Length Effect on Photoinduced Electron Transfer in Triad Rotaxane with Porphyrin [60]Fullerene, and Triphenylamine2010

    • Author(s)
      Sandanayaka, A. S. D., Sasabe, H., Araki, Y., 他4名
    • Journal Title

      Journal of Physical Chemistry A

      Volume: 114(16) Pages: 5242-5250

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of atomic nitrogen on the C_<60> cage2010

    • Author(s)
      Nikawa, H., Araki, Y., 他8名
    • Journal Title

      Chemical Communications (Cambridge, United Kingdom)

      Volume: 46(4) Pages: 631-633

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of Phe113 at the distal side of the heme domain of an oxygen-sensor(Ec DOS)in the characterization of the heme environment2009

    • Author(s)
      Ito, Shinya, Araki, Yasuyuki, Tanaka, Atsunari, Igarashi, Jotaro, Wada, Takehiko, Shimizu, Toru
    • Journal Title

      Journal of Inorganic Biochemistry 103

      Pages: 989-996

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Photophysical and Photovoltaic Properties of Porphyrin-Furan and-Thiophene Alternating Copolymers2009

    • Author(s)
      Umeyama, Tomokazu, Takamatsu, Takeshi, Tezuka, Noriyasu, Matano, Yoshihiro, Araki, Yasuyuki, Wada, Takehiko, Yoshikawa, Osamu, Sagawa, Takashi, Yoshikawa, Susumu, Imahori, Hiroshi
    • Journal Title

      The Journal of Physical Chemistry C 113

      Pages: 10798-10806

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Ellipticity-change-detected CD apparatus toward theapplication to the DNA/RNA conformational change2011

    • Author(s)
      Yasuyuki Araki, Makoto Murakami, Yoshiki Hamada, Seiji Sakamoto, Takehiko Wada
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry (ISNAC2011)
    • Place of Presentation
      Clark Memorial Student Senter, Hokkaido University, Sapporo, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] 円二色性の高感度検出へ向けた楕円率変化検出型円二色性測定法2011

    • Author(s)
      荒木保幸, 村上慎, 濱田芳生, 坂本清志, 和田健彦
    • Organizer
      2011年光化学討論会
    • Place of Presentation
      宮崎市河畔コンベンションセンター
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 楕円率変化検出による円二色測定法のフォトンカウンティング法による高感度化2011

    • Author(s)
      荒木保幸, 村上慎, 坂本清志, 和田健彦
    • Organizer
      第91回日本化学会春季年会
    • Place of Presentation
      神奈川大学横浜キャンパス
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Ellipticity-change-detected CD apparatus toward theapplication to the DNA/RNA conformational change2011

    • Author(s)
      Yasuyuki Araki, Makoto Murakami, Yoshiki Hamada, Seiji Sakamoto, Takehiko Wada
    • Organizer
      The 38^<th> International Symposium on Nucleic Acid Chemistry (ISNAC2011)
    • Place of Presentation
      Clark Memorial Student Senter, Hokkaido University, Sapporo, Japan
    • Related Report
      2010 Annual Research Report
  • [Presentation] トリフェニレンをコアとする亜鉛ポルフィリン6量体の不斉軸配位子による不斉誘起とその光励起挙動2010

    • Author(s)
      荒木保幸, Atula Sandanayaka, Rabbani Mohamed, 羽曾部卓, 村上慎, 坂本清志, 和田健彦
    • Organizer
      第90回日本化学会春季年会
    • Place of Presentation
      関西大学(大阪府)
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] New approach of time-resolved CD experiment for a biological application2010

    • Author(s)
      Y. Araki, M. Murakami, S. Sakamoto, T. Wada
    • Organizer
      Pacifichem2010
    • Place of Presentation
      Honolulu, Hawai, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] 不斉軸配位子による亜鉛ポルフィリン6量体の不斉誘起とその光励起挙動2010

    • Author(s)
      荒木保幸, 村上慎, 坂本清志, 和田健彦, SANDANAYAKA Atula, RABBANI Mohammad, 羽曾部卓
    • Organizer
      2010年光化学討論会
    • Place of Presentation
      千葉大学
    • Related Report
      2010 Final Research Report
  • [Presentation] 光励起をトリガーとするDNA・カチオン性ポルフィリン錯体の動的挙動変化の時間分解CD測定法を用いた解析2010

    • Author(s)
      村上慎, 荒木保幸, 坂本清志, 和田健彦
    • Organizer
      2010年光化学討論会
    • Place of Presentation
      千葉大学
    • Related Report
      2010 Final Research Report
  • [Presentation] TIME-RESOLVED CIRCULAR DICHROISM STUDY OF BINDING DYNAMICS OF H2TMPYP WITH DNA IN MICROSECOND TO MILLISECOND TIME-SCALE2010

    • Author(s)
      Yasuyuki Araki, Makoto Murakami, Seiji Sakamoto, Takehiko Wada
    • Organizer
      22nd International Symposium on Chirality
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] 高時間分解CD測定装置を用いた二重らせんDNA-porphyrin間相互作用解析(東北大多元研)2010

    • Author(s)
      村上慎, 荒木保幸, 坂本清志, 和田健彦
    • Organizer
      日本化学会第90春季年会(2010)
    • Place of Presentation
      近畿大学本部キャンパス
    • Related Report
      2010 Final Research Report
  • [Presentation] トリフェニレンをコアとする亜鉛ポルフィリン6量体の不斉軸配位子による不斉誘起とその光励起挙動(東北大多元研・北陸先端大)2010

    • Author(s)
      荒木保幸, Atula, Sandanayaka, Rabbani, Mohammad, 羽曾部卓, 村上慎, 坂本清志, 和田健彦
    • Organizer
      日本化学会第90春季年会(2010)
    • Place of Presentation
      近畿大学本部キャンパス
    • Related Report
      2010 Final Research Report
  • [Presentation] 不斉軸配位子による亜鉛ポルフィリン6量体の不斉誘起とその光励起挙動2010

    • Author(s)
      荒木保幸・村上慎・坂本清志・和田健彦・SANDANAYAKA Atula・RABBANI Mohammad・羽曾部卓
    • Organizer
      2010年光化学討論会
    • Place of Presentation
      千葉大学西千葉キャンパス
    • Related Report
      2010 Annual Research Report
  • [Presentation] New approach of time-resolved CD experiment for a biological application2010

    • Author(s)
      Araki, Y.; Murakami, M.; Sakamoto, S.; Wada, T.
    • Organizer
      Pacifichem 2010
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNAに結合したカチオン性ポルフィリンの時間分解円二色性測定による選択的検出2009

    • Author(s)
      荒木保幸, 村上慎, 坂本清志, 和田健彦
    • Organizer
      2009年光化学討論会
    • Place of Presentation
      群馬大学(群馬県)
    • Year and Date
      2009-09-18
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 円二色性測定装置2010

    • Inventor(s)
      荒木保幸、和田健彦、和田明生
    • Industrial Property Rights Holder
      日本分光株式会社
    • Industrial Property Number
      2010-190514
    • Filing Date
      2010-08-27
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 特許件2010

    • Inventor(s)
      荒木保幸、和田健彦、和田明生
    • Industrial Property Rights Holder
      日本分光株式会社
    • Industrial Property Number
      2010-190514
    • Filing Date
      2010-08-27
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi