Development of inhibitors based on short helical peptides for protein-protein interactions
Project/Area Number |
21750167
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Chemistry related to living body
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Research Institution | University of Toyama |
Principal Investigator |
FUJIMOTO Kazuhisa University of Toyama, 大学院・医学薬学研究部, 助教 (40334718)
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Project Period (FY) |
2009 – 2010
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Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | α-ヘリックス / タンパク間相互作用 / 阻害剤 / 生体高分子 |
Research Abstract |
I explored the effect of α-helical stabilization upon the binding of short peptides to DNAs. When a cross-linked peptide based on the homeodomain of transcription factor was titrated with a target DNA duplex, its dissociation constant (Kd) was calculated to be ca. 0.5 nM. This value was the double-digit smaller than that of the corresponding non-cross-linked peptide. The cross-linked peptide showed high substrate specificity for DNAs at the same level as the original DNA-binding protein.
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Report
(3 results)
Research Products
(51 results)
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[Journal Article] Photophysical Properties of 1,3,6,8-Tetrakis(arylethynyl)pyrenes with Donor or Acceptor Substituents: Their Fluorescence Solvatochromism and Lightfastness2009
Author(s)
Kazuhisa Fujimoto, Hisao Shimizu, Masaru Furusyo, Seiji Akiyama, Mio Ishida, Utako Furukawa, Toshiaki Yokoo, Masahiko Inouye
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Journal Title
Tetrahedron 65巻
Pages: 9357-9361
Related Report
Peer Reviewed
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[Journal Article] Characteristic Fluorescence Behavior of Dialkynylpyrene Derivatives in Hydrophobic Cavity of Protein2009
Author(s)
Shinmori, H., Furukawa, H., Fujimoto, K., Shimizu, H., Inouye, M., Takeuchi, T.
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Journal Title
Chem.Lett. 38
Pages: 84-85
NAID
Related Report
Peer Reviewed
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[Journal Article] Photophysical Properties of 1, 3, 6, 8-Tetrakis(arylethynyl)pyrenes with Donor or Acceptor Substituents : Their Fluorescence Solvatochromism and Lightfastness2009
Author(s)
Fujimoto, K., Shimizu, H., Frusyo, M.Akiyama, S., Ishida, M., Furukawa, U., Yokoo, T., Inouye, M.
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Journal Title
Tetrahedron 65
Pages: 9357-9361
Related Report
Peer Reviewed
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