Reading out the DNA sequence information by measuring the lifetime of the charge separated state

• Project/Area Number: 21750170
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Osaka University
• Principal Investigator: KAWAI Kiyohiko Osaka University, 産業科学研究所, 准教授 (50314422)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: DNA / 電子移動 / 光電荷分離 / 一塩基多型(SNPs) / 電荷分離 / 一塩基多型 / SNPs / ヨウ素デンプン反応

Research Abstract

Charge-separation and charge recombination in DNA was established using the fluorescent dyes those absorbs in the visible region. The lifetime of the charge-separated state was significantly affected by the surrounding sequences which enabled the read out the DNA sequence information based on the measurement of the lifetime of the charge separated state.

Research Products

• [Journal Article] Photocatalytic formation of I-I bonds using DNA which enables detection of single nucleotide polymorphisms (2010)
  • Author(s): K.Kawai, H.Kodera, T.Majima
  • Journal Title: J.Am.Chem.Soc. 132 Pages: 14216-14220
  • Peer Reviewed
• [Journal Article] 8-Oxo-7,8-dihydro-2'-deoxyguanosine produces a long-lived charge-separated state during the photosensitized one-electron oxidation of DNA resulting in efficient and exclusive degradation (2010)
  • Author(s): K.Kawai, E.Matsutani, T.Majima
  • Journal Title: Chem.Commun. 46 Pages: 3277-3279
  • Peer Reviewed
• [Journal Article] Sequence dependence of excess electron transfer in DNA (2010)
  • Author(s): K.Tainaka, M.Fujitsuka, T.Takada, K.Kawai, T.Majima
  • Journal Title: J.Phys.Chem.B 114 Pages: 14657-14663
  • Peer Reviewed
• [Journal Article] Charge separation and photosensitized damage in DNA mediated by naphthalimide, naphthaldiimide, and anthraquinone (2010)
  • Author(s): K.Kawai, Y.Osakada, E.Matsutani, T.Majima
  • Journal Title: J.Phys.Chem.B 114 Pages: 10195-10199
  • Peer Reviewed
• [Journal Article] Long-range charge transfer through DNA by replacing adenine with diaminopurine (2010)
  • Author(s): K.Kawai, H.Kodera, T.Majima
  • Journal Title: J.Am.Chem.Soc. 132 Pages: 627-630
  • Peer Reviewed
• [Journal Article] Photocatalytic formation of I-I bonds using DNA which enables detection of single nucleotide polymorphisms (2010)
  • Author(s): K.Kawai, H.Kodera, T.Majima
  • Journal Title: Journal of the American Chemical Society Volume: 132 Pages: 14216-1422
  • Peer Reviewed
• [Journal Article] Charge separation and photosensitized damage in DNA mediated by naphthalimide, naphthaldiimide, and anthraquinone (2010)
  • Author(s): K.Kawai, Y.Osakada, E.Matsutani, T.Majima
  • Journal Title: Journal of Physical Chemistry B Volume: 114 Pages: 10195-10199
  • Peer Reviewed
• [Journal Article] Sequence dependence of excess electron transfer in DNA (2010)
  • Author(s): K.Tainaka, M.Fujitsuka, T.Takada, K.Kawai, T.Majima
  • Journal Title: Journal of Physical Chemistry B Volume: 114 Pages: 14657-14663
  • Peer Reviewed
• [Journal Article] 8-Oxo-7, 8-dihydro-2'-deoxyguanosine produces a long-lived charge-separated state during the photosensitized one-electron oxidation of DNA resulting in efficient and exclusive degradation (2010)
  • Author(s): K.Kawai, E.Matsutani, T.Majima
  • Journal Title: Chemical Communications Volume: 46 Pages: 3277-3279
  • Peer Reviewed
• [Journal Article] Long-Range Charge Transfer through DNA by Replacing Adenine with Diaminopurine (2010)
  • Author(s): K.Kawai, H.Kodera, T.Majima
  • Journal Title: Journal of the American Chemical Society 132巻 Pages: 627-630
  • Peer Reviewed
• [Journal Article] Importance of protonation state of guanine radical cation during hole transfer in DNA (2009)
  • Author(s): K.Kawai, Y.Osakada, T.Majima
  • Journal Title: ChemPhysChem 10 Pages: 1766-1769
  • Peer Reviewed
• [Journal Article] Sequence-independent and rapid long-range charge transfer through DNA (2009)
  • Author(s): K.Kawai, H.Kodera, Y.Osakada, T.Majima
  • Journal Title: Nature Chem. 1 Pages: 156-159
  • Peer Reviewed
• [Journal Article] Sequence-independent and rapid long-range charge transfer through DNA (2009)
  • Author(s): K.Kawai, H.Kodera, Y.Osakada, T.Majima
  • Journal Title: Nature Chemistry 1巻 Pages: 156-159
  • Peer Reviewed
• [Journal Article] Importance of Protonation State of Guanine Radical Cation During Hole Transfer in DNA (2009)
  • Author(s): K.Kawai, Y.Osakada, T.Majima
  • Journal Title: ChemPhysChem 10巻 Pages: 1766-1769
  • Peer Reviewed
• [Journal Article] 電荷の移動速度からDNAの情報を読み出す (2009)
  • Author(s): 川井清彦
  • Journal Title: 化学と工業 62巻 Pages: 1088-1090
  • NAID: 10025548583
• [Journal Article] DNA内光電荷分離と光増感DNA損傷 (2000)
  • Author(s): 川井清彦
  • Journal Title: 光化学 40巻 Pages: 171-174
  • NAID: 10026051948
• [Presentation] 一分子レベル蛍光観測によるDNA内電荷分離寿命の測定 (2011)
  • Author(s): 川井清彦
  • Organizer: 日本化学会第91回春季年会
  • Place of Presentation: 横浜(神奈川大)
  • Year and Date: 2011-03-26
• [Presentation] 放射線および光照射によるDNA酸化損傷 (2011)
  • Author(s): 川井清彦
  • Organizer: 第42回基礎科学セミナー
  • Place of Presentation: 東海(原研)
  • Year and Date: 2011-01-17
• [Presentation] 放射線および光照射によるDNA酸化損傷 (2011)
  • Author(s): 川井清彦
  • Organizer: 第42回基礎科学セミナー
  • Place of Presentation: 東海(原研)(招待講演)
  • Year and Date: 2011-01-17
• [Presentation] Rapid long-range charge transfer through DNA (2010)
  • Author(s): 川井清彦
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii(USA)
  • Year and Date: 2010-12-18
• [Presentation] Rapid long-range charge transfer through DNA (2010)
  • Author(s): 川井清彦, 真嶋哲朗
  • Organizer: 2010International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii(USA)(招待講演)
  • Year and Date: 2010-12-18
• [Presentation] Kinetic studies of photosensitized one electron oxidation of DNA (2010)
  • Author(s): 川井清彦
  • Organizer: The 37th Internatinal Symposium on Nucleic Acids Chemistry 2010
  • Place of Presentation: 横浜
  • Year and Date: 2010-11-10
• [Presentation] ヨウ素デンプン反応による一塩基多型の検出 (2010)
  • Author(s): 川井清彦
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪(大阪大)
  • Year and Date: 2010-09-26
• [Presentation] 光触媒的ヨウ素生成反応を利用したDNA一塩基多型の検出 (2010)
  • Author(s): 川井清彦
  • Organizer: 2010年光化学討論会
  • Place of Presentation: 千葉(千葉大)
  • Year and Date: 2010-09-08
• [Presentation] 8-オキソグアニンはDNA内光電荷分離を促進し選択的に損傷する (2010)
  • Author(s): 川井清彦
  • Organizer: 第32回日本光医学・光生物学会
  • Place of Presentation: 東京(東京慈恵医大)
  • Year and Date: 2010-07-30
• [Presentation] 8-オキソグアニンはDNA内光電荷分離を促進し選択的に損傷する (2010)
  • Author(s): 川井清彦, 真嶋哲朗
  • Organizer: 第32回日本光医学・光生物学会
  • Place of Presentation: 東京慈恵会医科大学(東京都)
  • Year and Date: 2010-07-30
• [Presentation] Reading out the DNA sequence information by measuring the charge transfer kinetics (2010)
  • Author(s): 川井清彦
  • Organizer: Japan-China joint symposium on functional supramolecular architectures
  • Place of Presentation: Changchun (China)
  • Year and Date: 2010-07-27
• [Presentation] Reading out the DNA sequence information by measuring the charge transfer kinetics (2010)
  • Author(s): 川井清彦
  • Organizer: Japan-China joint symposium on functional supramolecular architectures
  • Place of Presentation: Changchun(China)(招待講演)
  • Year and Date: 2010-07-27
• [Presentation] 生体高分子DNA中の電荷移動 (2010)
  • Author(s): 川井清彦
  • Organizer: 第6回有機電子移動化学若手の会
  • Place of Presentation: 大阪(大阪府立大)
  • Year and Date: 2010-06-24
• [Presentation] 生体高分子DNA中の電荷移動 (2010)
  • Author(s): 川井清彦
  • Organizer: 第6回有機電子移動化学若手の会
  • Place of Presentation: 大阪(大阪府立大)(招待講演)
  • Year and Date: 2010-06-24
• [Presentation] Reading out the sequence information of DNA by measuring the charge transfer kinetics (2010)
  • Author(s): 川井清彦
  • Organizer: 杉山研研究室セミナー
  • Place of Presentation: 京都(京都大)
  • Year and Date: 2010-05-15
• [Presentation] Reading out the sequence information of DNA by measuring the charge transfer kinetics (2010)
  • Author(s): 川井清彦
  • Organizer: 杉山研研究室セミナー
  • Place of Presentation: 京都(京都大)(招待講演)
  • Year and Date: 2010-05-15
• [Presentation] デアザアデニン置換によるDNA内長距離電荷移動 (2010)
  • Author(s): 川井清彦
  • Organizer: 日本化学会第90春季年会(2010)
  • Place of Presentation: 近畿大学(大阪府)
  • Year and Date: 2010-03-26
• [Presentation] DNA内電荷移動速度の測定に基づくDNA配列情報の読み出し (2009)
  • Author(s): 川井清彦
  • Organizer: 第13回NAIST科学技術セミナー
  • Place of Presentation: 奈良先端大学(奈良県)
  • Year and Date: 2009-12-14
• [Presentation] DNA内電荷移動 光増感DNA損傷を速度定数に基づいて説明する (2009)
  • Author(s): 川井清彦
  • Organizer: 生体機能化学講演会
  • Place of Presentation: 兵庫県立大(兵庫県)
  • Year and Date: 2009-11-06
• [Presentation] Kinetic Studies of Long-Range Hole Transfer through DNA (2009)
  • Author(s): 川井清彦
  • Organizer: The Sixth International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 高山市民文化会館(岐阜県)
  • Year and Date: 2009-10-01
• [Presentation] Kinetic Studies of Long-Range Hole Transfer thmugh DNA (2009)
  • Author(s): Kiyohiko Kawai, Tetsuro Majima
  • Organizer: The Sixth International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 高山市民文化会館(岐阜県)
  • Year and Date: 2009-10-01
• [Presentation] 配列に依存しないDNA内高速ホール移動 (2009)
  • Author(s): 川井清彦
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 桐生市民文化会館(群馬県)
  • Year and Date: 2009-09-16
• [Presentation] DNA光増感一電子酸化過程における一重項酸素の発生 (2009)
  • Author(s): 川井清彦
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2009-09-14
• [Presentation] 光増感DNA一電子酸化損傷における一重項酸素の寄与 (2009)
  • Author(s): 川井清彦
  • Organizer: 第31回日本光医学・光生物学会
  • Place of Presentation: 梅田スカイビル(大阪府)
  • Year and Date: 2009-07-24
• [Presentation] DNA内電荷移動とDNA損傷の速度論的アプローチ (2009)
  • Author(s): 川井清彦
  • Organizer: 生体機能関連化学若手の会第21回サマースクール
  • Place of Presentation: 関西セミナーハウス(京都府)
  • Year and Date: 2009-07-13
• [Presentation] DNA内電荷移動とDNA損傷の速度論的アプローチ (2009)
  • Author(s): 川井清彦
  • Organizer: 生体機能関連化学 若手の会 第21回サマースクール
  • Place of Presentation: 関西セミナーハウス(京都府)
  • Year and Date: 2009-07-13
• [Book] ed by R.Naaman, Springer-Verlag, Inc., Berlin (2011)
  • Author(s): K.Kawai, T.Majima
  • Publisher: G-C Content independent long-range charge transfer through DNA, in "Topics in Current Chemistry : Electronic and magnetic properties of chiral molecules and supramolecular architectures"
• [Book] "G-C Content independent long-range charge transfer through DNA", in "Topics in Current Chemistry : Electronic and magnetic, properties of chiral molecules and supramolecular architectures" (2011)
  • Author(s): 川井清彦, 真嶋哲朗
  • Total Pages: 14
  • Publisher: Springer-Verlag, Inc., Berlin
• [Book] ed by Marc M.Greenberg, John Wiley & Sons, Inc., Hoboken, New Jersey, U.S.A (2009)
  • Author(s): K.Kawai, T.Majima
  • Publisher: Kinetics of long-range oxidative electron transfer through DNA in "Radicals and radicalion reactivity, in nucleic acid chemistry"
• [Book] ed by H. Kimura, A.Suzuki, Nova Science Publishers, Inc, Hauppauge, New York, U.S.A (2009)
  • Author(s): K.Kawai, T.Majima
  • Publisher: Consecutive adenine sequences serve as potential targets in photosensitized oxidative DNA damage in "New research on DNA damage"