Discrimination of methylated nucleobases by a novel modified DNA forming Schiff base

• Project/Area Number: 21750171
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Osaka University
• Principal Investigator: DOHNO Ghikara Osaka University, 産業科学研究所, 准教授 (60420395)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: バイオテクノロジー / メチル化核酸塩基 / クロスリンク / DNA / 分子認識 / 有機化学

Research Abstract

We have developed a novel chemistry-based method to detect all types of natural DNA methylation. A DNA probe functionalized with a formyl group discriminates between non-methylated and methylated nucleobases, such as 5-methylcytosine, N4-methylcytosine, and N6-methyladenine, by the formation of a selective interstrand cross-link.

Research Products

• [Journal Article] Ligand Inducible Assembly of a DNA Tetrahedron (2011)
  • Author(s): Dohno, C.代表, Atsumi, H.Nakatani, K.
  • Journal Title: Chem.Commun. 47 Pages: 3499-3501
  • Peer Reviewed
• [Journal Article] Ligand Inducible Assembly of a DNA Tetrahedron (2011)
  • Author(s): Dohno, C., et al.
  • Journal Title: Chem.Commun. Volume: 47 Pages: 3499-3501
  • Peer Reviewed
• [Journal Article] Discrimination of N6-methyl Adenine in a Specific DNA Sequence (2010)
  • Author(s): Dohno, C.代表, Shibata, T.Nakatani, K.
  • Journal Title: Chem.Commun. 46 Pages: 5530-5532
  • Peer Reviewed
• [Journal Article] Discrimination of N6-methyl Adenine in a Specific DNA Sequence (2010)
  • Author(s): Dohno, C., et al.
  • Journal Title: Chem.Commun. Volume: 46 Pages: 5530-5532
  • Peer Reviewed
• [Journal Article] The effect of linker length on binding affinity of a photoswitchable molecular glue for DNA (2009)
  • Author(s): C.Dohno, et.al.
  • Journal Title: Bioorg.Med.Chem. 17 Pages: 2536-2543
  • Peer Reviewed
• [Journal Article] Photoswitchable Unsymmetrical Ligand for DNA Hetero-Mismatches (2009)
  • Author(s): C.Dohno, et.al.
  • Journal Title: Eur.J.Org.Chem. Pages: 4051-4058
  • Peer Reviewed
• [Journal Article] A Light-Driven, Supramolecular Optical Switch (2009)
  • Author(s): S.Uno, et.al.
  • Journal Title: Angew.Chem.Int.Ed 48 Pages: 7362-7365
  • Peer Reviewed
• [Presentation] Photoswitchable molecular glue for DNA Nanotechnology (2011)
  • Author(s): Chikara Dohno, 代表、招待講演
  • Organizer: International Symposium Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules (ISSMA2011)
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2011-01-25
• [Presentation] Photoswitchable molecular glue for DNA Nanotechnology (2011)
  • Author(s): Chikara Dohno
  • Organizer: International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules(ISSMA2011)
  • Place of Presentation: 京都国際会議場(京都)(招待講演)
  • Year and Date: 2011-01-25
• [Presentation] Discrimination of methylated bases in the specific sequence (2010)
  • Author(s): 柴田知範、堂里主税,代表、中谷和彦
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacifichem2010)
  • Place of Presentation: ホノルル(米国)
  • Year and Date: 2010-12-17
• [Presentation] ホルミル基含有修飾核酸によるDNAメチル化の識別 (2010)
  • Author(s): 柴田知範
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学(大阪府)
  • Year and Date: 2010-03-28
• [Presentation] Adenine methylation-sensitive cross-link formation (2010)
  • Author(s): 柴田知範
  • Organizer: 第37回国際核酸化学シンポジウム(ISNAC2010)
  • Place of Presentation: はまぎんホール(横浜)
• [Presentation] Discrimination of methylated bases in the specific squence (2010)
  • Author(s): 柴田知範
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacifichem2010)
  • Place of Presentation: ホノルル(米国)
• [Presentation] DNA cross-link generated by a novel modified DNA containing a formyl group (2009)
  • Author(s): 柴田知範、堂里主税,代表, 中谷和彦
  • Organizer: The sixth International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 高山市民文化会館(岐阜県)
  • Year and Date: 2009-09-27
• [Presentation] 核酸二次構造安定性を大きく変化させる新規光応答性小分子リガンド (2009)
  • Author(s): 堂野主税
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2009-09-13