A search for peptides binding to assembly of amyloid-beta by novel fluorescence-screening

• Project/Area Number: 21750172
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Okayama University
• Principal Investigator: KITAMATSU Mizuki Okayama University, 大学院・自然科学研究科, 助教 (60379716)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ペプチド / 蛍光 / アミロイド / ペプチドライブラリー / アミロイドβ / 蛍光スペクトル / アミノ酸 / 蛍光アミノ酸 / アルツハイマー

Research Abstract

Many fluorescent dyes that fluoresces by exciting at light of various wavelength were connected to various peptides and these peptides were used as peptide library. These peptides were mixed with assembly of amyloid-beta in aqueous solution. Amyloid-beta is thought to be a causative agent of Alzheimer's disease. Of these fluorescent dye-modified peptides, the peptide bound to amyloid-beta strongly was found by fluorescence patterns of these fluorescent dyes modified to peptides.

Research Products

• [Journal Article] Prevention of adsorption of fluorescent amino acides to gels by using a peptide containinig dendritic amino acids. (2010)
  • Author(s): Mizuki Kitamatsu, Kazumasa Akagi, Masahiko Sisido.
  • Journal Title: Chemistry Letters 39 Pages: 1240-1241
  • Peer Reviewed
• [Journal Article] Prevention of adsorption of fluorescent amino acides to gels by using a peptide containinig dendritic amino acids. (2010)
  • Author(s): Takamitsu Morikawa, Mizuki Kitamatsu, Masahiko Sisido.
  • Journal Title: Peptide Science 2009 Pages: 369-370
• [Journal Article] Prevention of a dsorption of fluorescent amino acides to gels by using a peptide containinig dendritic amino acids (2010)
  • Author(s): Mizuki Kitamatsu, Kazumasa Akagi, Masahiko Sisido
  • Journal Title: Chemistry Letters Volume: 39 Pages: 1240-1241
  • Peer Reviewed
• [Journal Article] A search for peptides binding to assembly of amyloid-beta by novel fluorescence-screening (2010)
  • Author(s): Takamitsu Morikawa, Mizuki Kitamatsu, Masahiko Sisido
  • Journal Title: Peptide Science 2009 Pages: 369-370
• [Presentation] A Search for Peptides Binding to Assembly of Amyloid-Beta by Novel Fluorescence-Screening (2009)
  • Author(s): 北松瑞生
  • Organizer: 第46回ペプチド討論会
  • Place of Presentation: 福岡・北九州国際会議場
  • Year and Date: 2009-11-04
• [Remarks] ホームページ等
  • URL: http://www.biotech.okayama-u.ac.jp/labs/ohtsuki/kitamatsu.pdf
• [Patent(Industrial Property Rights)] 新規分岐状アミノ酸、新規分岐状アミノ酸と蛍光性アミノ酸の複合体 (2010)
  • Inventor(s): 北松瑞生
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Number: 2010-216789
  • Filing Date: 2010-09-28
• [Patent(Industrial Property Rights)] 新規アミロイドβ凝集体結合性ペプチドおよび新規アミロイドβ凝集体結合性ペプチドを用いたアミロイド病の検査方法 (2009)
  • Inventor(s): 北松瑞生
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Number: 2009-206771
  • Acquisition Date: 2009-09-08
• [Patent(Industrial Property Rights)] 新規アミロイドβ凝集体結合性ペプチドおよび新規アミロイドβ凝集体結合性ペプチドを用いたアミロイド病の検査方法 (2009)
  • Inventor(s): 北松瑞生
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Number: 2009-206771
  • Filing Date: 2009-09-08