Analysis of the basement membrane function and application to the artificial basement membrane

• Project/Area Number: 21750174
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: HOZUMI Kentaro Tokyo University of Pharmacy and Life Science, 薬学部, 助教 (10453804)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ラミニン / 基底膜 / 細胞接着 / 細胞外マトリックス / インテグリン / 細胞生物学 / 生体材料 / 再生医学 / 細胞・組織 / シグナル伝達

Research Abstract

Molecular dissection of laminin by synthetic peptides was tested and found that biological activities of laminin could analyze and mimic using laminin active peptides. The laminin derived mixed peptides-polysaccharides exhibited many biological activities and these activities can control by changing the biological and physical properties of peptides-polysaccharides. Combinations of the peptides and the polysaccharides provide unique peptide-matrices with different biological activities, suggesting that these constructs are useful for cell type specific biomaterials.

Research Products

• [Journal Article] Biological activity of laminin peptide conjugated alginate and chitosan matrices (2011)
  • Author(s): 山田雄二、保住建太郎、片桐文彦、吉川大和、野水基義
  • Journal Title: Biopolymers Vol.94 Pages: 711-720
  • Peer Reviewed
• [Journal Article] Biological activity of laminin peptide conjugated alginate and chitosan matrices. (2011)
  • Author(s): 山田雄二、保住建太郎, 他
  • Journal Title: Biopolymers Volume: 94 Pages: 711-720
  • Peer Reviewed
• [Journal Article] Cell surface receptor-specific scaffold requirements for adhesion to laminin-derived peptide-chitosan membranes (2010)
  • Author(s): 保住建太郎、小田切大、山田雄二、佐々木彩乃、藤森力、若井優紀、内田達也、片桐文彦、吉川大和、野水基義
  • Journal Title: Biomaterials Vol.31 Pages: 3237-3243
  • Peer Reviewed
• [Journal Article] Cell adhesive peptide screening of the mouse laminin alpha1 chain G domain (2010)
  • Author(s): 保住建太郎、秋月種康、山田雄二、原俊博、漆畑俊哉、片桐文彦、吉川大和、野水基義
  • Journal Title: Archive of Biochemistry and Biophysics Vol.503 Pages: 213-222
  • Peer Reviewed
• [Journal Article] Syndecan- and integrin-binding peptides synergistically accelerated cell adhesion (2010)
  • Author(s): 保住建太郎、小林一樹、片桐文彦、吉川大和、門谷裕一、野水基義
  • Journal Title: FEBS Letters Vol.534 Pages: 3381-3385
  • Peer Reviewed
• [Journal Article] Identification of alpha-dystroglycan binding sequences in the laminin alpha2 chain LG4-5 module (2010)
  • Author(s): 鈴木喜晴、保住建太郎、漆畑俊哉、吉村大志、吉川大和、Gumerson JD、Michele DE、Hoffman MP、山田吉彦、野水基義
  • Journal Title: Matrix Biology Vol.29 Pages: 143-151
  • Peer Reviewed
• [Journal Article] Syndecan- and integrin-binding peptides synergistically accelerated cell adhesion (2010)
  • Author(s): 保住建太郎、野水基義, 他
  • Journal Title: FEBS Letters Volume: 534 Pages: 3381-3385
  • Peer Reviewed
• [Journal Article] Cell adhesive peptide screening of the mouse laminin α1 chain G domain. (2010)
  • Author(s): 保住建太郎、野水基義, 他
  • Journal Title: Arch.Biochem.Biophys. Volume: 503 Pages: 213-222
  • Peer Reviewed
• [Journal Article] Cell surface receptor-specific scaffold requirements for adhesion to laminin-derived peptide-chitosan membranes. (2010)
  • Author(s): 保住建太郎、野水基義, 他
  • Journal Title: Biomaterials Volume: 31 Pages: 3237-3243
  • Peer Reviewed
• [Journal Article] Identification of alpha-dystroglycan binding sequences in the laminin alpha2 chain LG4-5 module. (2010)
  • Author(s): 鈴木喜晴、保住建太郎, 等
  • Journal Title: Matrix Biology 29 Pages: 143-151
  • Peer Reviewed
• [Journal Article] Design and activity of multifunctional fibrils using receptor-specific small peptides (2009)
  • Author(s): 大賀有希子、片桐文彦、竹山一基、保住建太郎、吉川大和、西則雄、野水基義
  • Journal Title: Biomaterials Vol.30 Pages: 6731-6738
  • Peer Reviewed
• [Journal Article] Chain-specific heparin binding sequences in the laminin alpha chain LG45 modules (2009)
  • Author(s): 保住建太郎、鈴木喜晴、内山喜彦、片桐文彦、吉川大和、野水基義
  • Journal Title: Biochemistry Vol.48 Pages: 5375-5381
  • Peer Reviewed
• [Journal Article] Chain-specific heparin binding sequences in the laminin alpha chain LG45 modules. (2009)
  • Author(s): 保住建太郎、野水基義, 等
  • Journal Title: Biochemistry 48 Pages: 5375-5381
  • Peer Reviewed
• [Journal Article] Design and activity of multifunctional fibrils using receptor-specific small peptides. (2009)
  • Author(s): 大賀有希子、保住建太郎, 等
  • Journal Title: Biomaterials 30 Pages: 6731-6738
  • Peer Reviewed
• [Presentation] 異なるサブタイプのインテグリン間相互作用は細胞接着活性を減少させる (2011)
  • Author(s): 保住建太郎, 等
  • Organizer: 第13回生命科学研究会シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2011-01-07
• [Presentation] Integrin crosstalk of fibroblasts on peptides conjugated chitosan membrane (2010)
  • Author(s): 保住建太郎, 等
  • Organizer: The American Society For Cell Biology 50th Annual Meeting
  • Place of Presentation: Philadelphia(米国)
  • Year and Date: 2010-12-12
• [Presentation] Development of functional biomaterials using laminin active peptides (2010)
  • Author(s): 保住建太郎
  • Organizer: 5th International Peptide Symposium
  • Place of Presentation: 京都
  • Year and Date: 2010-12-05
• [Presentation] Subtype specific integrin crosstalk of fibroblasts on mixed ECM derived integrin binding peptides-chitosan membranes (2010)
  • Author(s): 保住建太郎, 等
  • Organizer: American Society for Matrix Biology Biennial Meeting 2010
  • Place of Presentation: Charleston(米国)
  • Year and Date: 2010-10-25
• [Presentation] Subtype specific integrin crosstalk of fibroblasts on mixed ECM derived integrin binding peptides-chitosan membranes (2010)
  • Author(s): 保住建太郎、野水基義, 他
  • Organizer: American Society for Matrix Biology Bienial Meeting 2010
  • Place of Presentation: Charleston(米国)
  • Year and Date: 2010-10-25
• [Presentation] ペプチドーキトサン膜における細胞表面受容体の種類と足場特性の相関関係 (2010)
  • Author(s): 保住建太郎, 等
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-07
• [Presentation] 混合ペプチドーキトサン膜を用いた異なるインテグリン間相互作用の解析 (2010)
  • Author(s): 保住建太郎, 等
  • Organizer: 第42回日本結合組織学会学術大会・第53回マトリックス研究会大会合同学術集会
  • Place of Presentation: 秋田
  • Year and Date: 2010-08-20
• [Presentation] 混合ペプチドーキトサン膜を用いた異なるインテグリン間相互作用の解析 (2010)
  • Author(s): 保住建太郎、野水基義, 他
  • Organizer: 第42回日本結合組織学会・第53回マトリックス研究会 合同各術集会
  • Place of Presentation: 秋田
  • Year and Date: 2010-08-20
• [Presentation] Subtype specific integrin crosstalk on laminin derived integrin binding peptide-chitosan membranes (2010)
  • Author(s): 保住建太郎, 等
  • Organizer: Gordon Research Conference (ECM and signal transduction)
  • Place of Presentation: Biddeford(米国)
  • Year and Date: 2010-06-28
• [Presentation] Laminin active peptides conjugated on chitosan membrane as a cell adhesive matrix (2009)
  • Author(s): 保住建太郎, 等
  • Organizer: 第49回米国細胞生物学会
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2009-12-05
• [Presentation] Laminin active peptides conjugated on chitosan membrane as a cell adhesive matrix英語ポスター発表 (2009)
  • Author(s): 保住建太郎、野水基義, 等
  • Organizer: 第49回米国細胞生物学会
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2009-12-05
• [Presentation] Mixed peptide chitosan membranes are multifunctional and can mimic the activities of laminin alpha1 chain LG4 protein (2009)
  • Author(s): 保住建太郎, 等
  • Organizer: 第3回アジア環太平洋ペプチドシンポジウム
  • Place of Presentation: 済州島(韓国)
  • Year and Date: 2009-11-09
• [Presentation] Mixed peptide chitosan membranes are multifunctional and can mimic the activities of laminin alphal chain LG4 protein.英語口頭発表 (2009)
  • Author(s): 保住建太郎、野水基義, 等
  • Organizer: 第3回アジア環太平洋ペプチドシンポジウム
  • Place of Presentation: 済州島(韓国)
  • Year and Date: 2009-11-09
• [Presentation] Mixed syndecanand integrin-binding peptides accelerates cell adhesion through the synergetic interactions (2009)
  • Author(s): 保住建太郎, 等
  • Organizer: 第42回ペプチド討論会
  • Place of Presentation: 北九州
  • Year and Date: 2009-11-04
• [Presentation] Mixed syndecan- and integrin-binding peptides accelerates cell adhesion through the synergetic interactions.英語口頭発表 (2009)
  • Author(s): 保住建太郎、野水基義, 等
  • Organizer: 第42回ペプチド討論会
  • Place of Presentation: 北九州
  • Year and Date: 2009-11-04
• [Presentation] 異なる細胞表面受容体間のシナジェティック効果を誘起する医用材料の開発 (2009)
  • Author(s): 保住建太郎, 等
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本
  • Year and Date: 2009-09-16
• [Presentation] 異なる細胞表面受容体間のシナジェティック効果を誘起する医用材料の開発口頭発表 (2009)
  • Author(s): 保住建太郎、野水基義, 等
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本
  • Year and Date: 2009-09-16
• [Presentation] Peptide-chitosan membranes can mimic the biological activities of laminin alpha1 LG4 module (2009)
  • Author(s): 保住建太郎, 等
  • Organizer: 第8回環太平洋結合組織シンポジウム
  • Place of Presentation: 葉山
  • Year and Date: 2009-06-04
• [Presentation] Peptide-chitosan membranes can mimic the biological activities of laminin alphal LG4 module.英語口頭発表 (2009)
  • Author(s): 保住建太郎、野水基義, 等
  • Organizer: 第8回環太平洋結合組織シンポジウム
  • Place of Presentation: 葉山
  • Year and Date: 2009-06-04
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