Development of novel Ends-Quencher-Free molecular beacon for DNA sequence analysis

• Project/Area Number: 21750176
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Nihon University
• Principal Investigator: SAITO Yoshio Nihon University, 工学部, 准教授 (40385985)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: DNA / 蛍光プローブ / モレキュラービーコン

Research Abstract

We synthesized various substituted fluorescent deoxyadenosine derivatives. Among them cyano-substituted deoxyguanosine analog showed a remarkable solvatochromicity. Such environmentally sensitive solvatochromic fluorescent adenosine analog may be useful for sequence specific DNA detection with high efficiency.

Research Products

• [Journal Article] Singly and doubly labeled base-discriminating fluorescent oligonucleotide probes containing oxo-pyrene chromophore (2010)
  • Author(s): S.S.Bag, R.Kundu, K.Matsumoto, Y.Saito, I.Saito
  • Journal Title: Bioorg.Med.Chem.Lett. 20 Pages: 3227-3230
  • Peer Reviewed
• [Journal Article] Synthesis of Novel Push-Pull-Type Solvatochromic 2'-Deoxyguanosine Derivatives with Longer Wavelength Emission (2010)
  • Author(s): Y.Saito^*, A.Suzuki, K.Imai, N.Nemoto, I.Saito^*
  • Journal Title: Tetrahedron Lett. 51 Pages: 2606-2609
  • Peer Reviewed
• [Journal Article] Singly and doubly labeled base-discriminating fluorescent oligonucleotide probes containing oxo-pyrene chromophore (2010)
  • Author(s): S.S.Bag, R.Kundu, K.Matsumoto, Y.Saito, I.Saito
  • Journal Title: Bioorg.Med.Chem.Lett. Volume: 20 Pages: 3227-3230
  • Peer Reviewed
• [Journal Article] Synthesis of Novel Push-Pull Type Solvatochromic 2'-Deoxyguanosine Derivatives with Longer Wavelength Emission (2010)
  • Author(s): Y.Saito, A.Suzuki, K.Imai, N.Nemoto, I.Saito
  • Journal Title: Tetrahedron Lett. Volume: 51 Pages: 2606-2609
  • Peer Reviewed
• [Journal Article] A Probe Containing Two Base-discriminating Fluorescent (BDF) Nucleosides for SNP Typjng (2010)
  • Author(s): Y.Yoshida, K.Niwa, K.Yamada, M.Tokeshi, Y.Baba, Y.Saito. A.Okamoto, I.Saito
  • Journal Title: Chemistry Letters 39巻 Pages: 116-117
  • Peer Reviewed
• [Journal Article] Photo-switching of vinylpyrene-substituted 2'-deoxyguanosine and its application (2009)
  • Author(s): Y.Saito, Y.Takeuchi, K.Matsumoto, N.Takahashi, A.Suzuki, I.Saito
  • Journal Title: Nueleic Acids Symp.Ser. 53巻 Pages: 193-194
• [Presentation] 遺伝子診断のための蛍光DNAプローブの開発 (2010)
  • Author(s): 齋藤義雄
  • Organizer: 第32回日本光医学・光生物学会(受賞講演)
  • Place of Presentation: 東京
  • Year and Date: 2010-07-21
• [Presentation] 遺伝子診断のための蛍光DNAプローブの開発 (2010)
  • Author(s): 齋藤義雄
  • Organizer: 第32回日本光医学・光生物学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-21
• [Presentation] 遺伝子診断のための画期的なモルキュラービーコンの開発 (2009)
  • Author(s): 齋藤義雄、齋藤烈
  • Organizer: 第31回日本光医学・光生物学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-24
• [Patent(Industrial Property Rights)] C8位置換プリン塩基誘導体及びそれを利用した蛍光プローブ (2009)
  • Inventor(s): 齋藤烈、齋藤義雄、高橋尚弥
  • Industrial Property Rights Holder: 日本大学
  • Industrial Property Number: 2009-188664
  • Filing Date: 2009-08-17