Optically controlled gel-microtool manipulated by optical tweezers for on-chip micromanipulation

• Project/Area Number: 21760182
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Nagoya University (2010); Tohoku University (2009)
• Principal Investigator: MAUYAMA Hisataka Nagoya University, 工学研究科, 助教 (60377843)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: マイクロ・ナノデバイス / 光ピンセット / マイクロ流体デバイス / マイクロツール / 光制御 / マイクロ・ナノ操作 / 計測

Research Abstract

We studied on optically-controlled gel-microtool fot on-chip micromanipulation. We achieved (1) Bidirectional cell adhesion control of the gel-microtool induced by UV/VIS illumination using Spiropyran, (2) Intracellular measurement using the gel-microsensor injected using the optically-controlled lipofection by optical pH regulation using Leuco crystal violet.

Research Products

• [Journal Article] Optical Adhesion Control of Hydrogel Microtools for On-Demand Immobilization and Measurement of Cells on a Microfluidic Chip (2010)
  • Author(s): Hisataka Maruyama, Toshio Fukuda, Fumihito Arai
  • Journal Title: Journal of Robotics and Mechatronics Vol.22 Pages: 631-638
  • Peer Reviewed
• [Journal Article] Optical Adhesion Control of Hydrogel Microtools for On-Demand Immobilization and Measurement of Cells on a Microfluidic Chip (2010)
  • Author(s): Hisataka Maruyama, Toshio Fukuda, Fumihito Arai
  • Journal Title: Journal of Robotics and Mechatronics Volume: 22 Pages: 631-638
  • Peer Reviewed
• [Presentation] Nagoya University, Japan, Ayae Honda, Tatsuro Takahata, Fumihito Arai, Optical pH Regulation Using Functional Nanotool Impregnating with Photo-Responsive Chemical for Intracellular Measurement (2010)
  • Author(s): Hisataka Maruyama, Taisuke Masuda, Naoya Inoue
  • Organizer: MHS2010
  • Place of Presentation: Noyori Conference Hall, Nagoya
  • Year and Date: 2010-11-09
• [Presentation] Optical pH Regulation Using Functional Nanotool Impregnating with Photo-Responsive Chemical for Intracellular Measurement (2010)
  • Author(s): Hisataka Maruyama, Taisuke Masuda, Naoya Inoue, Nagoya University, Japan, Ayae Honda, Tatsuro Takahata, Fumihito Arai
  • Organizer: 日本機械学会ロボティクス・メカトロニクス講演会2010
  • Place of Presentation: Noyori Conference Hall, Nagoya
  • Year and Date: 2010-11-09
• [Presentation] OPTICAL INJECTION AND MANIPULATION OF FUNCTIONAL NAOTOOL USING PHOTO-RESPONSIVE CHEMICAL AND OPTICALTWEEZERS FOR INTRACELLULAT MEASUREMENT (2010)
  • Author(s): Hisataka Maruyama, Kyosuke Kotani, Ayae Honda, Tatsuro Takahata, Fumihito Arai
  • Organizer: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Martiniplaza, Groningen, The Netherlands
  • Year and Date: 2010-10-06
• [Presentation] OPTICAL INJECTION AND MANIPULATION OF FUNCTIONAL NAO TOOL USING PHOTO-RESPONSIVE CHEMICAL AND OPTICALTWEE ZERS FOR INTRACELLULAT MEASUREMENT (2010)
  • Author(s): Hisataka Maruyama, Kyosuke Kotani, Ayae Honda, Tatsuro Takahata, Fumihito Arai
  • Organizer: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Martiniplaza, Groningen, The Netherlands
  • Year and Date: 2010-10-06
• [Presentation] Injection and Laser Manipulation of Nanotool Using Photo Responsive Chemical for Intracellular Measurement (2010)
  • Author(s): Hisataka Maruyama, Kyosuke Kotani, Ayae Honda, Tatsuro Takahata, Fumihito Arai
  • Organizer: IEEE NANO 2010
  • Place of Presentation: KINTEXT, Seoul, Korea
  • Year and Date: 2010-08-19
• [Presentation] オンチップ細胞計測に関する研究-その2:細胞内計測のためのナノツールの細胞導入と細胞内レーザ操作- (2010)
  • Author(s): 丸山央峰, 港谷恭輔, 吉田圭佑, 本田文江, 高畑辰郎, 新井史人
  • Organizer: 日本機械学会ロボティクス・メカトロニクス講演会2010
  • Place of Presentation: 旭川大雪アリーナ(北海道)
  • Year and Date: 2010-06-16
• [Presentation] 機能性マイクロツールを用いた細胞近傍環境計測 (2009)
  • Author(s): 丸山央峰, 大竹智之, 新井史人
  • Organizer: 第10回システムインテグレーション部門講演会
  • Place of Presentation: 東京,芝浦工業大学
  • Year and Date: 2009-12-26
• [Presentation] 機能性マイクロツールを用いた細胞近傍環境計測 (2009)
  • Author(s): 丸山央峰, 大竹智之, 新井史人
  • Organizer: 第10回システムインテグレーション部門講演会
  • Place of Presentation: 東京, 芝浦工業大学
  • Year and Date: 2009-12-26
• [Presentation] Fabrication of Functional Gel-Nanotool for Intracellular Measurement (2009)
  • Author(s): Hisataka Maruyama, Fumihito Arai
  • Organizer: 2009 International Symposium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: 名古屋,名古屋大学
  • Year and Date: 2009-11-10
• [Presentation] Fabrication of Functional Gel-Nanotool for Intracellular Measurement (2009)
  • Author(s): 丸山央峰, 新井史人
  • Organizer: 2009 International Symposium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: 名古屋, 名古屋大学
  • Year and Date: 2009-11-10
• [Presentation] 細胞内環境計測のための機能性ナノツールの作製 (2009)
  • Author(s): 丸山央峰, 港谷恭輔, 飯塚龍, 本田文江, 江島美穂, 新井史人
  • Organizer: 第27回日本ロボット学会学術講演会
  • Place of Presentation: 横浜,横浜国立大学
  • Year and Date: 2009-09-16
• [Presentation] 細胞内環境計測のための機能性ナノツールの作製 (2009)
  • Author(s): 丸山央峰, 港谷恭輔, 飯塚龍, 本田文江, 江島美穂, 新井史人
  • Organizer: 第27回日本ロボット学会学術講演会
  • Place of Presentation: 横浜, 横浜国立大学
  • Year and Date: 2009-09-16
• [Remarks] ホームページ等
  • URL: http://www.biorobotics.mech.nagoyau.ac.jp/research/Robomec2010/Robomec2010_maruyama_cell.pdf
• [Remarks]
  • URL: http://www.biorobotics.mech.nagoyau.ac.jp/research/21stCheminas/21Cheminas_maruyama.pdf