A NADH-dependent fiber-optic biosensor with a UV-LED excitation system
| Project/Area Number |
21760310
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Measurement engineering
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MIYAJIMA Kumiko Tokyo Medical and Dental University, 生体材料工学研究所, 技術職員 (10516298)
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| Research Collaborator |
MITSUBAYASHI Kohji 東京医科歯科大学, 生体材料工学研究所, 教授 (40307236)
KUDO Hiroyuki 東京医科歯科大学, 生体材料工学研究所, 講師 (70329118)
ARAKAWA Takahiro 東京医科歯科大学, 生体材料工学研究所, 助教 (50409637)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | バイオセンサ / 高輝度紫外線LED / NADH / アルコール脱水素酵素 / 光ファイバ / 還元型ニコチンアミドアデニンジヌクレオチド / 光ファイバプローブ / エタノールガス / ニコチンアミドアデニンジヌクレオチド / エタノール光ファイバプローブ |
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| Research Abstract |
A fiber optic bio-sniffer for high-selective alcohol vapor monitoring with an UV-LED was constructed and tested. In order to achieve high selectivity, the sensor utilizes fluorescence of nicotineamide adenine dinucleotide (NADH), which is produced by the enzymatic reaction of alcohol dehydrogenase. The bio-sniffer was fabricated by attaching a wet enzyme immobilized membrane to the fiber-optic NADH measurement system. The fluorescent intensity significantly increased with a stable value related to the ethanol gas concentration when ethanol vapor was exposed.
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Report
(3 results)
Research Products
(48 results)
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[Journal Article] Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae (Der f1)2011
Author(s)
K.Miyajima, G.Itabashi, T.Koshida, K.Tamari, D.Takahashi, T.Arakawa, H.Kudo, H.Saito, K.Yano, K.Shiba, K.Mitsubayashi
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Journal Title
Environmental Monitoring and Assessment in press
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[Journal Article] Soft contact lens biosensor for in-situ monitoring of tear glucose as non-invasive blood sugar assessment2011
Author(s)
M.X.Chu, K.Miyajima, D.Takahashi, T.Arakawa, K.Sano, S.Sawada, H.Kudo, Y.Iwasaki, K.Akiyoshi, M.Mochizuki, K.Mitsubayashi
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Journal Title
Talanta 83
Pages: 960-965
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[Journal Article] Soft contact lens biosensor for in-situ monitoring of tear glucose as non-invasive blood sugar assessment2011
Author(s)
MingXing Chu, Kumiko Miyajima, Daishi Takahashi, Takahiro Arakawa, Kenji Sano, Shin-ichi Sawada, Hiroyuki Kudo, Yasuhiko Iwasaki, Kazunari Akiyoshi, Manabu Mochizuki, Kohji Mitsubayashi
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Journal Title
Talanta
Volume: 83
Pages: 960-965
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Peer Reviewed
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[Presentation] 生体適合性ポリマーを用いたシート型化学センサに関する研究2009
Author(s)
白井隆行, 工藤寛之, 初明星, 宮島久美子, 齊藤浩一, 森本展行, 矢野和義, 岩崎泰彦, 秋吉一成, 三林浩二
Organizer
第48回日本生体医工学会大会
Place of Presentation
タワーホール船堀, 東京
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[Presentation] A biochemical sensor for toluene using enzyme inhibition of butyrylcholi nesterase2009
Author(s)
Hirokazu Saito, Yuki Suzuki, Tomoko Gessei, Elito Kazawa, Kumiko Miyajima, Daishi Takahashi, Takahiro Arakawa, Hiroyuki Kudo, Kohji Mitsubayashi
Organizer
9th Workshop on(Bio)sensors and Bioanalytical microtechniques in environmental and clinical analysis
Place of Presentation
Universite de Montreal, Montreal, Canada
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[Book] 展望とトピックス(光ファイバー技術を利用してダニアレルゲンをリアルタイムで測定,第71回分析化学討論会)2010
Author(s)
宮島久美子, 板橋玄, 玉利佳子, 高橋大志, 荒川貴博, 工藤寛之, 齊藤浩一, 矢野和義, 芝紀代子, 三林浩二
Publisher
社団法人 日本分析化学会 展望とトピックス委員会(東京)
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