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Investigation of the dielectrophoretic properties of parasitic bacteria in emulsion and construction of new separation and detection systems of bacteria

Research Project

Project/Area Number 21760317
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Measurement engineering
Research InstitutionTokyo Metropolitan University

Principal Investigator

UCHIDA Satoshi  Tokyo Metropolitan University, 大学院・理工学研究科, 准教授 (90305417)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords計測システム / 誘電泳動 / 乳濁液 / 細菌 / 油滴 / 静電容量 / 薬剤耐性 / 泳動速度 / 数値解析
Research Abstract

In the present study, the dielectrophoretic properties of parasitic bacteria in emulsion were investigated, and new separation and measurement apparatus was developed for rapid and simple detection. Optimum electrode arrangement and separation condition from oil droplet were obtained by numerical analyses of electric field distribution in microcell, and dielectrophoretic velocity and trajectory of bacteria. In actual, selective condensation was achieved for various kinds of bacteria. Moreover, the bacterial amount was estimated quantitatively by combination of dielectrophoretic micro filter and LCR meter.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (9 results)

All 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Soft-lithographic methods for fabrication of dielectrophoretic devices using molds by proton beam writing2010

    • Author(s)
      Y.Shiine, H.Nishikawa, Y.Furuta, K.Kanamitsu, T.Satoh, Y.Ishii, T.Kamiya, R.Nakao, S.Uchida
    • Journal Title

      Microelectronic Engineering Vol.87

      Pages: 835-838

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Soft-lithographic methods for fabrication of dielectrophoretic devices using molds by proton beam writing2010

    • Author(s)
      Y.Shiine, H.Nishikawa, Y.Furuta, K.Kanamitsu, T.Satoh, Y.Ishii, T.Kamiya, R.Nakao, S.Uchida
    • Journal Title

      Microelectronic Engineering

      Volume: 87 Pages: 835-838

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 誘電泳動を用いたエマルション中の混入菌に対する分離濃縮2011

    • Author(s)
      泉雄太, 内田諭, 杤久保文嘉, 内山明弘
    • Organizer
      平成23年電気学会全国大会
    • Place of Presentation
      大阪大学(大阪)
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Optical Counting of Trapped Bacteria in Dielectrophoretic Device with Pillar Array2010

    • Author(s)
      S.Uchida, R.Nakao, Y.Shiine, H.Nishikawa
    • Organizer
      World Automation Congress 2010
    • Place of Presentation
      神戸国際会議場(神戸)
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 誘電泳動デバイスの菌捕集特性における電極配置の影響2010

    • Author(s)
      星野祐太朗, 白井直機, 内田諭, 杤久保文嘉
    • Organizer
      第34回静電気学会全国大会
    • Place of Presentation
      鳥取大学(鳥取)
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 飲料混入菌の泳動濃縮及び画像計数2010

    • Author(s)
      高瀬亜希, 内田諭
    • Organizer
      日本食品工学会第11回年次大会
    • Place of Presentation
      東京海洋大学(東京)
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 微小流路内における細菌の誘電泳動速度計測2009

    • Author(s)
      西村僚太, 内田諭, 杤久保文嘉
    • Organizer
      第33回静電気学会全国大会
    • Place of Presentation
      東京都市大学(東京)
    • Related Report
      2010 Final Research Report
  • [Presentation] 微小流路内における細菌の誘電泳動速度計測2009

    • Author(s)
      西村僚太, 内田諭, 栃久保文嘉
    • Organizer
      第34回静電気学会全国大会
    • Place of Presentation
      東京都市大学(東京)
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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