Fabrication of Enzyme-Nanoparticle Complexes Exhibiting High Enzymatic Activity in Organic Solvents

• Project/Area Number: 21760620
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Reaction engineering/Process system
• Research Institution: Kyushu University
• Principal Investigator: MATSUNE Hideki Kyushu University, 工学研究院, 助教 (10380586)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 材料合成プロセス / 酵素 / 磁性粒子 / 酵素反応 / 有機溶媒 / 磁性ナノ粒子 / 表面修飾 / シリカ / 親水/疎水二層構造 / 有機溶媒での利用 / ナノファイバー / ナノバイオ / 高分子修

Research Abstract

I found a new method to promote enzymatic reactions even in organic solvents. Enzyme was embedded in an organic layer that was prepared on nanostructures such as magnetic nanoparticles and nanowires. The organic layer has a bilayer structure consisting of inner hydrophilic and outer hydrophobic properties. The embedded enzyme showed a high activity even after the long-term immersion of the enzyme-nanoparticle complex in organic solvent, because the hydrophilic environment of the organic layer would protect from the deactivation of enzyme.

Research Products

• [Journal Article] Synthesis and Properties of Nanocapsule with Shell of Amphiphilic Layer (2011)
  • Author(s): Hideki Matsune, Daisuke Sakurai, Ozora Baba, Sakae Takenaka, Masahiro Kishida
  • Journal Title: Proceedings of China and Japan Symposium on Chemical Engineering in press
• [Journal Article] Chemical Synthesis of Silver Nanowires using N, N-Dimethyldodecylamine Oxide (2010)
  • Author(s): Hideki Matsune, Yudai Kuramitsu, Sakae Takenaka, Masahiro Kishida
  • Journal Title: Chem.Lett. 39巻 Pages: 717-719
  • NAID: 10027182565
  • Peer Reviewed
• [Journal Article] Chemical Synthesis of Silver Nanowires Using N,N-Dimethyldodecylamine (2010)
  • Author(s): Hideki Matsune, et al.
  • Journal Title: Chemistry Letters Volume: 39 Pages: 717-719
  • Peer Reviewed
• [Journal Article] New Synthetic Method of Silver Nanowires Using N,N-Dimethyldodecylamine (2010)
  • Author(s): Hideki Matsune, et al.
  • Journal Title: Kyushu University Global-COE program Science for Future Molecular Systems Journal Volume: 4 Pages: 40-41
• [Journal Article] Preparation of Carbon Nanotube-Supported Pt Catalysts Covered with Silica Layers ; Application to Cathode Catalysts for PEFC (2010)
  • Author(s): HIROSHI MATSUMORI, et al.
  • Journal Title: Applied Catalysis A : General Volume: 373 Pages: 176-185
  • Peer Reviewed
• [Journal Article] Sintering Resistance and Catalytic Activity of Platinum Nanoparticles Covered with a Microporous Silica Layer using Methyltriethoxysilane (2010)
  • Author(s): KEIZO NAKAGAWA, et al.
  • Journal Title: Catalysis Letters Volume: 136 Pages: 71-76
  • Peer Reviewed
• [Journal Article] Improvement in the Durability of Carbon Nanotube-Supported Ruthenium Catalysts by Coverage with Silica Layers (2010)
  • Author(s): TAKAFUMI ARIKE, et al.
  • Journal Title: Bulletin of Chemical Society of Japan Volume: 83 Pages: 953-959
  • Peer Reviewed
• [Journal Article] Preparation of Carbon-Supported Pt-Co Alloy Nanoparticles for Oxygen Reduction Reaction : Promotion of Pt-Co Alloy Formation by Coverage with Silica (2010)
  • Author(s): SAKAE TAKENAKA, et al.
  • Journal Title: Chemistry Letters Volume: 39 Pages: 458-459
  • Peer Reviewed
• [Journal Article] Preparation of Supported Pt-Co Alloy Nanoparticle Catalysts for the Oxygen Reduction Reaction by Coverage with Silica (2010)
  • Author(s): SAKAE TAKENAKA, et al.
  • Journal Title: Journal of Catalysis Volume: 274 Pages: 228-238
  • Peer Reviewed
• [Journal Article] Preparation of Silica-Coated Pt-Ni Alloy Nanoparticles using Microemulsion and Formation of Carbon Nanofibers by Ethylene Decomposition (2010)
  • Author(s): Keizo NAKAGAWA, et al.
  • Journal Title: Studies in Surface Science and Catalysis Volume: 175 Pages: 793-796
  • Peer Reviewed
• [Journal Article] Synthesis of Hollow Nanospheres Consisting of Amphiphilic Block Copolymers (2009)
  • Author(s): Hideki Matsune, Daisuke Sakurai, Sakae Takenaka, Masahiro Kishida
  • Journal Title: Chem.Lett. 38巻 Pages: 746-747
  • NAID: 10025124546
  • Peer Reviewed
• [Journal Article] Synthesis of Hollow Nanospheres Consisting of Amphiphilic Block Copolymers (2009)
  • Author(s): Hideki MATSUNE, et al
  • Journal Title: Chemistry Letters 38 Pages: 746-747
  • NAID: 10025124546
  • Peer Reviewed
• [Journal Article] Excellent Durability of Carbon-Supported Pt Cathode Catalysts Covered with Silica Layers (2009)
  • Author(s): Hiroshi MATSUMORI, et al
  • Journal Title: ECS Transaction 25 Pages: 689-696
  • Peer Reviewed
• [Journal Article] Formation of Carbon Nanotubes through Ethylene Decomposition over Supported Pt Catalysts and Silica-Coated Pt Catalysts (2009)
  • Author(s): Sakae TAKENAKA, et al
  • Journal Title: Carbon 47 Pages: 1251-1257
  • Peer Reviewed
• [Presentation] 両親媒性分子の架橋による高分子ナノカプセルの合成とその特性 (2011)
  • Author(s): 松根英樹, et al.
  • Organizer: 化学工学会 第76年会
  • Place of Presentation: 東京農業工業大学(小金井市)
  • Year and Date: 2011-03-22
• [Presentation] 担持有機金属錯体触媒のシリカ被覆およびその反応特性 (2011)
  • Author(s): 宮本幸太郎, et al.
  • Organizer: 化学工学会 第76年会
  • Place of Presentation: 東京農業工業大学(小金井市)
  • Year and Date: 2011-03-22
• [Presentation] Ptナノ粒子用担体としてのカーボンナノファイバーの開発 (2011)
  • Author(s): 朴佳榮, et al.
  • Organizer: 化学工学会 第76年会
  • Place of Presentation: 東京農業工業大学(小金井市)
  • Year and Date: 2011-03-22
• [Presentation] 光ピンセットを用いた新規浮遊培養法の構築と新しい細胞特性の解明への応用 (2011)
  • Author(s): 松根英樹, et al
  • Organizer: 高分子学会九州支部フォーラム
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2011-03-12
• [Presentation] アルカン燃焼反応におけるPt触媒の担体効果およびシリカ被覆効果 (2011)
  • Author(s): 渡辺雄太, et al.
  • Organizer: 第13回化学工学会学生発表会(神戸大会)【西日本地区】
  • Place of Presentation: 神戸大学(神戸市)
  • Year and Date: 2011-03-05
• [Presentation] 両親媒性分子の架橋による高分子ナノカプセルの合成とその特性 (2011)
  • Author(s): 松根英樹, 櫻井大輔, 馬場大空, 竹中壮, 岸田昌浩, 北堀かずみ, 藤井優子
  • Organizer: 化学工学会第76年会
• [Presentation] New Synthesis Method for Silver Nanowires Using N,N-dimethyldodecylamine N-Oxide (2010)
  • Author(s): Yudai Kuramitsu, et al.
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: ハワイコンベンションセンター(米国ハワイ州)
  • Year and Date: 2010-12-20
• [Presentation] Functionalization of carbon nanofibers for preparation of highly dispersed metal nanoparticles (2010)
  • Author(s): 伊賀淳朗, et al.
  • Organizer: 第23回化学工学に関する国際シンポジウム
  • Place of Presentation: 九州産業大学(福岡市)
  • Year and Date: 2010-12-04
• [Presentation] カーボンナノファイバー担持Ptナノ粒子触媒の調製 (2010)
  • Author(s): 伊賀淳朗, et al.
  • Organizer: 2010日本化学会西日本大会
  • Place of Presentation: 熊本大学(熊本市)
  • Year and Date: 2010-11-07
• [Presentation] PEFC用カーボンナノチューブ担持Ptアノード触媒のシリカ被覆によるCO被毒耐性の向上 (2010)
  • Author(s): 岡本幸宏, et al.
  • Organizer: 2010日本化学会西日本大会
  • Place of Presentation: 熊本大学(熊本市)
  • Year and Date: 2010-11-07
• [Presentation] Chemical Synthesis of Silver Nanowires Using Tertiary Amine N-Oxides (2010)
  • Author(s): 松根英樹, et al
  • Organizer: 9th Korea-Japan Symposium on Materials & Interface
  • Place of Presentation: Ocean Resort Hotel(大韓民国麗水市)
  • Year and Date: 2010-11-01
• [Presentation] シリカ被覆炭素担持Pd触媒のPEFCカソードへの応用-シリカ層の最適化- (2010)
  • Author(s): 宮本拓明, et al.
  • Organizer: 第106回触媒討論会
  • Place of Presentation: 山梨大学(甲府市)
  • Year and Date: 2010-10-24
• [Presentation] シリカナノ粒子表面におけるリビングラジカル重合反応と高分子ナノカプセルの合成 (2010)
  • Author(s): 馬場大空, et al.
  • Organizer: 第3回化学工学3支部合同徳島大会
  • Place of Presentation: 徳島大学(徳島市)
  • Year and Date: 2010-10-24
• [Presentation] ドデシルジメチルアミンオキシドを用いた銀ナノワイヤーの調製 (2010)
  • Author(s): 増田恭三, et al
  • Organizer: 第3回化学工学3支部合同徳島大会
  • Place of Presentation: 徳島大学(徳島市)
  • Year and Date: 2010-10-24
• [Presentation] シリカ被覆によるカーボンナノチューブ担持Pd触媒の溶出耐性向上 (2010)
  • Author(s): 宮本拓明, et al
  • Organizer: 化学工学会 第42回秋季大会
  • Place of Presentation: 同志社大学 今出川キャンパス(京都市)
  • Year and Date: 2010-09-08
• [Presentation] 複合ナノ粒子の液相合成と二元金属触媒への応用 (2010)
  • Author(s): 田中啓之, et al.
  • Organizer: 化学工学会 第42回秋季大会
  • Place of Presentation: 同志社大学 今出川キャンパス(京都市)
  • Year and Date: 2010-09-08
• [Presentation] 常圧乾燥法による低密度シリカエアロゲルの調製 (2010)
  • Author(s): 沼田健吾, et al
  • Organizer: 化学工学会 第42回秋季大会
  • Place of Presentation: 同志社大学 今出川キャンパス(京都市)
  • Year and Date: 2010-09-08
• [Presentation] Study on complete methane oxidation nn alumina-supported Pt and Cr_2O_3 catalysts (2010)
  • Author(s): Mardwita, et al.
  • Organizer: 化学工学会 第42回秋季大会
  • Place of Presentation: 同志社大学 今出川キャンパス(京都市)
  • Year and Date: 2010-09-08
• [Presentation] PEFC用炭素担持Pt-Co合金触媒のシリカ被覆による活性化 (2010)
  • Author(s): 竹中壮, et al.
  • Organizer: 化学工学会 第42回秋季大会
  • Place of Presentation: 同志社大学 今出川キャンパス(京都市)
  • Year and Date: 2010-09-08
• [Presentation] Synthesis and applying for Photocatalyst of Carbon nanotubes covered with TiO_2 nanoparticles (2010)
  • Author(s): 有家隆文, et al.
  • Organizer: The Sixth Tokyo Conference on Advanced Catalytic Science and Technology (TOCTAT6) & The Fifth Asia Pacific Congress on Catalysis (APCAT5)
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2010-07-23
• [Presentation] Improved durability of carbon-supported Pt catalysts for cathode in PEFCs by coverage with silica ; Application of carbon black to support for Pt (2010)
  • Author(s): 松森裕史, et al.
  • Organizer: The Sixth Tokyo Conference on Advanced Catalytic Science and Technology (TOCTAT6) & The Fifth Asia Pacific Congress on Catalysis (APCAT5)
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2010-07-23
• [Presentation] Carbon nanotube-supported Pd catalysts covered with silica for cathode in PEFC (2010)
  • Author(s): 竹中壮, et al.
  • Organizer: The Sixth Tokyo Conference on Advanced Catalytic Science and Technology (TOCTAT6) & The Fifth Asia Pacific Congress on Catalysis (APCAT5)
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2010-07-23
• [Presentation] The Influence of Organic Reagents Addition on the Catalytic Activity of Supported Cobalt Catalysts for Carbon Monooxide Hydrogenation (2010)
  • Author(s): Mardwita, et al.
  • Organizer: Inagural (1st) International Conference on Molecular & Functional Catalysis (ICMFC-1)
  • Place of Presentation: Resorts World Sentosa Convention Centre(シンガポール)
  • Year and Date: 2010-07-15
• [Presentation] 高分子ナノカプセル用原料のリビングラジカル重合 (2010)
  • Author(s): 馬場大空, et al
  • Organizer: 第47回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場(北九州市)
  • Year and Date: 2010-07-10
• [Presentation] CO被毒耐性をもつシリカ被覆カーボンナノチューブ担持Pt電極触媒の開発 (2010)
  • Author(s): 岡本幸宏, et al
  • Organizer: 第47回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場(北九州市)
  • Year and Date: 2010-07-10
• [Presentation] New Synthesis Method for Silver Nanowires Using N, N-dimethyl dodecylamine N-Oxide (2010)
  • Author(s): Yudai Kuramitsu, Hideki Matsune, Takenaka Sakae, Masahiro Kishida
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
• [Presentation] Chemical Synthesis of Silver Nanowires Using Tertiary Amine N-Oxides (2010)
  • Author(s): Hideki Matsune, Yudai Kuramitsu, Kyoso Masuda, Sakae Takenaka, Masahiro Kishida
  • Organizer: 9^th Korea-Japan Symposium on Materials & Interfaces
• [Presentation] ドデシルジメチルアミンオキシドを用いた銀ナノワイヤーの調製 (2010)
  • Author(s): 増田恭三, 倉光雄大, 松根英樹, 竹中壮, 岸田昌浩
  • Organizer: 第3回化学工学3支部合同徳島大会
• [Presentation] シリカナノ粒子表面におけるリビングラジカル重合反応と高分子ナノカプセルの合成 (2010)
  • Author(s): 馬場大空, 櫻井大輔, 松根英樹, 竹中壮, 岸田昌浩
  • Organizer: 第3回化学工学3支部合同徳島大会
• [Presentation] 高分子ナノカプセル用原料のリビングラジカル重合 (2010)
  • Author(s): 馬場大空, 竹中壮, 松根英樹, 岸田昌浩
  • Organizer: 第47回化学関連支部合同九州大会
• [Presentation] New Route for Synthesis of Silver Nanowires (2010)
  • Author(s): Yudai Kuramitsu, Hideki Matsune, Takenaka Sakae, Masahiro Kishida
  • Organizer: The 2010 Global COE International Symposium on Future Molecular Systems-Beyond Supramolecular Chemistry
• [Presentation] ドデシルジメチルアミンオキシドを用いた銀ナノ構造体の調製 (2010)
  • Author(s): 増田恭三, 竹中壮, 松根英樹, 岸田昌浩
  • Organizer: 第12回化学工学会学生発表会(福岡大会)
  • Place of Presentation: 西日本地区
• [Presentation] 酵素固定化微粒子の開発と有機溶媒中での反応 (2009)
  • Author(s): 松根英樹, 竹中壮, 岸田昌浩
  • Organizer: 化学工学会第41回秋季大会
  • Year and Date: 2009-09-17
• [Presentation] 酵素固定化微粒子の開発と有機溶媒中での反応 (2009)
  • Author(s): 松根英樹, et al
  • Organizer: 化学工学会 第41回秋季大会
  • Place of Presentation: 広島大学(東広島市)
  • Year and Date: 2009-09-07
• [Presentation] New synthesis method for silver nanowires by use of alkylamine-N-oxide (2009)
  • Author(s): 倉光雄大, 松根英樹, 竹中壮, 岸田昌浩
  • Organizer: 第21回化学工 学に関する国際シンポジウム
• [Presentation] アミンオキシドを用いた銀ナノワイヤーの新規合成法の開発 (2009)
  • Author(s): 倉光雄大, 松根英樹, 竹中壮, 岸田昌浩
  • Organizer: 日本化学会西日本大会 2009
• [Presentation] Synthesis and Extraction of Hollow Nanospheres Consisting of Amphiphilic Block Copolymers (2009)
  • Author(s): 櫻井大輔, 松根英樹, 竹中壮, 岸田昌浩
  • Organizer: The 2009 Global COE International Symposium for Future Molecular Systems
  • Place of Presentation: Fukuoka, Japan
• [Presentation] アルキルアミンN-オキサイドを用いた銀ナノワイヤーの合成 (2009)
  • Author(s): 倉光雄大, 松根英樹, 竹中壮, 岸田昌浩
  • Organizer: 化学工学会第41回秋季大会
• [Presentation] Preparation of Nanocapsule with DoubleLayer for Particle Synthesis Process (2009)
  • Author(s): 櫻井大輔, 松根英樹, 竹中壮, 岸田昌浩
  • Organizer: 第5回日中化工シンポジウム
• [Book] 若手研究者の大きな可能性を秘めた研究開発 (2009)
  • Author(s): 松根英樹, 櫻井大輔, 竹中壮, 岸田昌浩
  • Publisher: 化学工学会九州支部若手ケミカルエンジニア連絡会
• [Book] Molecular Nano Dynamics 2 (2009)
  • Author(s): Hideki MATSUNE, et al
  • Total Pages: 14
  • Publisher: Wiley-VCH
• [Book] 若手研究者の大きな可能性を秘めた研究開発 (2009)
  • Author(s): Hideki MATSUNE, et al
  • Total Pages: 5
  • Publisher: 化学工学会九州支部若手エンジニア連絡会
• [Remarks] ホームページ
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002595/research.html
• [Remarks]
  • URL: http://hyoka.ofe.kyushu-u.ac.jp/search/details/K002595/research.html