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Development of techniques for membrane protein assembly into nano vesicles using structural proteins

Research Project

Project/Area Number 21760635
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

YOSHINO Tomoko  Tokyo University of Agriculture and Technology, 大学院・工学研究院, 特任准教授 (30409750)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords小胞 / 構造タンパク質 / 細胞 / 遺伝子融合 / 融合タンパク質 / ナノバイオ / 磁性細菌
Research Abstract

In this study, the techniques for production of "target molecule-assembled nano vesicles" using structural proteins have been developed. W succeeded in forming vesicles and integrating proteins onto the vesicles using Mms13, which was isolated as a protein tightly bound to bacterial magnetic particles. Furthermore, the method for purification of the vesicles form cell homogenates have been established, and a novel tool was developed toward the analysis of target molecules.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (18 results)

All 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (11 results)

  • [Journal Article] Inducible expression of transmembrane proteins on bacterial magnetic particles in Magnetospirillum magneticum AMB-12010

    • Author(s)
      Tomoko Yoshino, Akiko Shimojo, Yoshiaki Maeda, Tadashi matsunaga
    • Journal Title

      Appl.Environ.Microbiol 76(4)

      Pages: 1152-1157

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bioengineering of Bacterial Magnetic Particles and their Applications in Biotechnology2010

    • Author(s)
      Tomoko Yoshino, Yoshiaki Maeda, Tadashi Matsunaga
    • Journal Title

      Recent Patents on Biotechnology 4(2)

      Pages: 214-225

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 磁性ビーズを利用した生体分子の分析技術2010

    • Author(s)
      田中剛、吉野知子、松永是
    • Journal Title

      ぶんせき 3

      Pages: 133-138

    • NAID

      10026786815

    • Related Report
      2010 Final Research Report
  • [Journal Article] Bioengineering of bacterial magnetic particles and their applications in biotechnology2010

    • Author(s)
      Tomoko Yoshino, et al.
    • Journal Title

      Recent Patents on Biotechnology

      Volume: 4 Pages: 214-225

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inducible expression of transmembrane proteins on bacterial magnetic particles in Magnetospirillum magneticum AMB-12010

    • Author(s)
      Tomoko Yoshino, et al.
    • Journal Title

      Appl.Environ.Microbiol. 76

      Pages: 1152-1157

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nano-sized bacterial magnetic particles displaying pyruvate phosphate dikinase for pyrosequencing2009

    • Author(s)
      Tomoko Yoshino, Taisei Nishimura, Tetsushi Mori, Shigeya Suzuki, Hideki Kambara, Haruko Takeyama, Tadashi matsunaga
    • Journal Title

      Biotechnol.Bioeng. 103

      Pages: 130-137

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nano-sized bacterial magnetic particles displaying pyruvate phosphate dikinase for pyrosequencing2009

    • Author(s)
      Tomoko Yoshino, et al.
    • Journal Title

      Biotechnol.Bioeng. 103

      Pages: 130-137

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 磁性細菌ホストの改変によるバイオナノ磁性粒子上への外来タンパク質発現量の向上と機能評価2011

    • Author(s)
      鐘築由香・吉野知子・松永是
    • Organizer
      日本化学会第91春季年会(講演予稿集発行日)(講演予稿集)
    • Year and Date
      2011-03-11
    • Related Report
      2010 Final Research Report
  • [Presentation] 磁性細菌ホストの改変によるバイオナノ磁性粒子上への外来タンパク質発現量の向上と機能評価2011

    • Author(s)
      吉野知子, 他2名
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      (日本化学会第91春季年会(2011)講演予稿集)
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 医療応用を目指したバイオナノ磁性粒子の分子設計2010

    • Author(s)
      吉野知子
    • Organizer
      第7回農工大・電通大合同シンポジウム
    • Place of Presentation
      電気通信大学
    • Year and Date
      2010-12-11
    • Related Report
      2010 Final Research Report
  • [Presentation] 医療応用を目指したバイオナノ磁性粒子の分子設計2010

    • Author(s)
      吉野知子
    • Organizer
      第7回農工大・電通大合同シンポジウム
    • Place of Presentation
      電気通信大学(東京都)(依頼講演)
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] バイオナノ磁性粒子を用いた血清中の自己抗体検出技術の開発2010

    • Author(s)
      吉野知子、内山諒、米山健太郎、堀部卓郎、久原基樹、松永是
    • Organizer
      第50回化学センサ研究会
    • Place of Presentation
      神奈川工科大学
    • Year and Date
      2010-09-02
    • Related Report
      2010 Final Research Report
  • [Presentation] バイオナノ磁性粒子を用いた血清中の自己抗体検出技術の開発2010

    • Author(s)
      吉野知子, 他5名
    • Organizer
      第50回化学センサ研究会
    • Place of Presentation
      神奈川工科大学(神奈川県)
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Nanocomposites Consisting of in vivo-biotinylated Bacterial Magnetic Particles and Quantum Dots for Magnetic and Fluorescent Labeling of Cancer Cells.2009

    • Author(s)
      Yoshiaki Maeda, Tomoko Yoshino, Tadashi Matsunaga
    • Organizer
      19 th MRS-J Symposium
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] バイオナノ磁性粒子膜上への効率的な膜タンパク質ディスプレイに向けた発現誘導システムの構築2009

    • Author(s)
      吉野知子, 下条明子, 前田義昌, 松永是
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] バイオナノ磁性粒子膜上への効率的な膜タンパク質ディスプレイに向けた発現誘導システムの構築2009

    • Author(s)
      吉野知子, 他3名
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] バイオナノ磁性粒子上への嗅覚受容体ディスプレイに向けた発現方法の検討2009

    • Author(s)
      鳴田知沙・近藤有美子・前田義昌・吉野知子・河野芳明・松永是
    • Organizer
      第12回バイオテクノロジー部会シンポジウム
    • Place of Presentation
      九州大学
    • Year and Date
      2009-09-15
    • Related Report
      2010 Final Research Report
  • [Presentation] バイオナノ磁性粒子上への嗅覚受容体ディスプレイに向けた発現方法の検討2009

    • Author(s)
      吉野知子, 他5名
    • Organizer
      第12回バイオテクノロジー部会シンポジウム
    • Place of Presentation
      九州大学(福岡県)
    • Year and Date
      2009-09-15
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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