Oxidized lipid metabomics by online-supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry
| Project/Area Number |
21760637
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
BAMBA Takeshi Osaka University, 工学研究科, 准教授 (10432444)
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| Co-Investigator(Renkei-kenkyūsha) |
FUKUSAKI Eiichiro 大阪大学, 工学研究科, 教授 (40273594)
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| Research Collaborator |
YOSHIDA Masaru 神戸大学, 医学研究科, 准教授
SHINOHARA Masakazu 神戸大学, 医学研究科, グローバルCOE研究員
USHA Acharya University of Massachusetts Medical School(USA), Assistant Professor
MATSUBARA Atsuski 大阪大学, 工学研究科, 博士後期課程
UCHIKATA Takato 大阪大学, 工学研究科, 博士後期課程
LEE Jae Won 大阪大学, 工学研究科, 博士後期課程
YAMAMOTO Takashi 大阪大学, 工学研究科, 博士前期期課程
WADA Yuusuke 大阪大学, 工学研究科, 博士前期期課程
SANDA Hotaka 大阪大学, 工学研究科, 博士前期期課程
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 超臨界流体 / 超臨界流体クロマトグラフィー / 超臨界流体抽出 / 質量分析 / メタボロミクス / リピドミクス / 脂質 / 酸化脂質 / メタポロミクス |
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| Research Abstract |
A supercritical fluid (SCF) possesses features such as low viscosity and high diffusivity. Therefore, it has suitable properties for use as a mobile phase in chromatography and a media in extraction. Supercritical fluid chromatography (SFC) would be possible to perform rapid and high-resolution separation of complicate metabolites which is difficult to separate by GC and HPLC. Supercritical fluid extraction (SFE) is mild and high-efficient, and it is suitable for unstable metabolites by solvent extraction. Therefore, to apply them to biometrics analysis, it would be necessary to establish analytical system using unique character of SCF. In this study, we have developed the technology toward application of online SFE-SFC/MS system to oxidized lipid profiling and applied the analysis system to lipidomics in various diseases.
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Report
(3 results)
Research Products
(79 results)
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[Journal Article] SphingosineKinases and their metabolites modulate endolysosomal trafficking in photoreceptoers.2011
Author(s)
Yonamine I., Bamba, T., Nirala, N., Jesmin, N., Kosakowska-Cholody, T., Nagashima, K., Fukusaki, E., Acharya, J.K., Acharya, U.,
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Journal Title
J.Cell Biol. 192(4)
Pages: 557-567
Related Report
Peer Reviewed
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[Journal Article] SphingosineKinases and their metabolites modulate endolysosomal trafficking in photoreceptors.2011
Author(s)
Yonamine, I., Bamba, T, Nirala, N., Jesmin, N., Kosakowska-Cholody, T., Nagashima, K., Fukusaki, E., Acharya, J.K., Acharya, U.
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Journal Title
J.Cell Biol.
Volume: 192(4)
Pages: 557-567
Related Report
Peer Reviewed
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[Journal Article] CDase is a pan-ceramidase in Drosophila.2010
Author(s)
Yuan, C., Rao, R.P., Jesmin, N.,Bamba, T., Nagashima, K., Pascual, A., Preat, T., Fukusaki, E., Acharya, U., Acharya, J.K.
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Journal Title
Mol.Biol.Cell. 22(1)
Pages: 33-43
Related Report
Peer Reviewed
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[Journal Article] CDase is a pan-ceramidase in Drosophila.2010
Author(s)
Yuan, C, Rao, R.P., Jesmin, N., Bamba, T., Nagashima, K., Pascual, A., Preat, T., Fukusaki, E., Acharya, U., Acharya, J.K.
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Journal Title
Mol.Biol.Cell.
Volume: 22(1)
Pages: 33-43
Related Report
Peer Reviewed
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