Establishment of a novel chicken monoclonal antibody generation system for applying to antibody drug development.
Project/Area Number |
21760641
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Biofunction/Bioprocess
|
Research Institution | Okayama University |
Principal Investigator |
MAGARI Masaki Okayama University, 大学院・自然科学研究科, 助教 (50359882)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | モノクローナル抗体 / ハイブリドーマ / ニワトリ / 抗体医薬 / DT40 |
Research Abstract |
Monoclonal antibodies have recently proven to be research reagents and biopharmaceuticals. Thus, it has become increasingly important to develop technologies to obtain a desired antibody. In this study, I aim to establish a novel system for generating chicken monoclonal antibody. To end this, I establish a novel fusion partner cell line from chicken B cell line DT40 by targeted disruption of immunoglobulin and thymidine kinase genes.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation.2010
Author(s)
Kajita, M., Okazawa, T., Ikeda, M., Todo, K., Magari, M., Kanayama, N., Ohmori, H.
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Journal Title
J.Biosci.Bioeng. 110
Pages: 351-358
NAID
Related Report
Peer Reviewed
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[Journal Article] Enhancement of hypermutation frequency in the chicken B cell line DT40 for efficient diversification of the antibody repertoire.2010
Author(s)
Magari, M., Kanehiro, Y., Todo, K., Ikeda, M., Kanayama, N., Ohmori, H.
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Journal Title
Biochem.Biophys.Res.Commun. 396
Pages: 353-358
Related Report
Peer Reviewed
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[Journal Article] Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increaseing point mutation2010
Author(s)
Kajita, M., Okazawa, T., Ikeda, M., Todo, K., Magari, M., Kanayama, N., Ohmori, H.
-
Journal Title
J.Biosci.Bioeng.
Volume: 110
Pages: 351-358
NAID
Related Report
Peer Reviewed
-
[Journal Article] Enhancement of hypermutation frequency in the chicken B cell line DT40 for efficient diversification of the antibody repatoire2010
Author(s)
Magari, M., Kanehiro, Y., Todo, K., Ikeda, M., Kanayama, N., Ohmori, H.
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Journal Title
Biochem.Biophys.Res.Commun.
Volume: 396
Pages: 353-358
Related Report
Peer Reviewed
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[Presentation] Affinity maturation of a mouse monoclonal antibody in an in vitro antibody generation system using the hypermutating chicken B cell line2010
Author(s)
N.Kanayama, S.Kojima, S.Fujii, K.Kitamura, K.Inoue, N.Okayama, S.Matsuda, Y.Kanehiro, K.Todo, M.Magari, H.Ohmori
Organizer
14^<th> International Congress of Immunology
Place of Presentation
Kobe
Related Report
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