Establishment of a novel chicken monoclonal antibody generation system for applying to antibody drug development.

• Project/Area Number: 21760641
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Okayama University
• Principal Investigator: MAGARI Masaki Okayama University, 大学院・自然科学研究科, 助教 (50359882)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: モノクローナル抗体 / ハイブリドーマ / ニワトリ / 抗体医薬 / DT40

Research Abstract

Monoclonal antibodies have recently proven to be research reagents and biopharmaceuticals. Thus, it has become increasingly important to develop technologies to obtain a desired antibody. In this study, I aim to establish a novel system for generating chicken monoclonal antibody. To end this, I establish a novel fusion partner cell line from chicken B cell line DT40 by targeted disruption of immunoglobulin and thymidine kinase genes.

Research Products

• [Journal Article] Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation. (2010)
  • Author(s): Kajita, M., Okazawa, T., Ikeda, M., Todo, K., Magari, M., Kanayama, N., Ohmori, H.
  • Journal Title: J.Biosci.Bioeng. 110 Pages: 351-358
  • NAID: 110007730029
  • Peer Reviewed
• [Journal Article] Enhancement of hypermutation frequency in the chicken B cell line DT40 for efficient diversification of the antibody repertoire. (2010)
  • Author(s): Magari, M., Kanehiro, Y., Todo, K., Ikeda, M., Kanayama, N., Ohmori, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 396 Pages: 353-358
  • Peer Reviewed
• [Journal Article] Conditional transformation of immunoglobuline mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line. (2010)
  • Author(s): Kajita, M., Magari, M., Todo, K., Kanayama, N., Ohmori, H.
  • Journal Title: J.Biosci.Bioeng. 107 Pages: 206-209
  • NAID: 110007618907
  • Peer Reviewed
• [Journal Article] Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increaseing point mutation (2010)
  • Author(s): Kajita, M., Okazawa, T., Ikeda, M., Todo, K., Magari, M., Kanayama, N., Ohmori, H.
  • Journal Title: J.Biosci.Bioeng. Volume: 110 Pages: 351-358
  • NAID: 110007730029
  • Peer Reviewed
• [Journal Article] Enhancement of hypermutation frequency in the chicken B cell line DT40 for efficient diversification of the antibody repatoire (2010)
  • Author(s): Magari, M., Kanehiro, Y., Todo, K., Ikeda, M., Kanayama, N., Ohmori, H.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 396 Pages: 353-358
  • Peer Reviewed
• [Journal Article] Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line (2010)
  • Author(s): Kajita, M., Magari, M., Todo, K., Kanayama, N., Ohmori, H.
  • Journal Title: J.Biosci.Bioeng. Volume: 109 Pages: 407-410
  • NAID: 110007618907
  • Peer Reviewed
• [Journal Article] Conditional transformation of immunoglobulin mutation pattern from gene conversion intopoint mutation by controlling XRCC3 expression in the DT40 B cell line (2010)
  • Author(s): Kajita, M., Magari, M., et.al.
  • Journal Title: J.Biosci.Bioeng. 109 Pages: 407410-407410
  • NAID: 110007618907
  • Peer Reviewed
• [Presentation] Affinity maturation of a mouse monoclonal antibody in an in vitro antibody generation system using the hypermutating chicken B cell line (2010)
  • Author(s): N.Kanayama, S.Kojima, S.Fujii, K.Kitamura, K.Inoue, N.Okayama, S.Matsuda, Y.Kanehiro, K.Todo, M.Magari, H.Ohmori
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
• [Presentation] DT40-SWを用いるin vitro抗体作製システムの高機能化:遺伝子変換と点突然変異の転換システムの構築 (2009)
  • Author(s): 曲正樹、梶田真道、金山直樹、大森斉
  • Organizer: 第61回日本生物工学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-24