| Project/Area Number |
21760645
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Research Collaborator |
ITOU Keisuke 静岡県立大学, 食品栄養科学部, 助教 (40580460)
HOSONO Hideo 名古屋大学, 大学院・生命農学研究科, 教授 (00237348)
KOJIMA Takaaki 名古屋大学, 大学院・生命農学研究科, 助教 (40509080)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | バイオテクノロジー / プロテオーム / ゲノム / 蛋白質 / 酵素 / 蛋白質間相互作用 / 機能インタラクトーム解析 / 逆プロテオミクス解析 / 酵母2ハイブリッド系 / 定量的大規模スクリーニング / 進化分子工学 / 創薬探索技術 / 蛋白質生産 / 染色体分配 |
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| Research Abstract |
An efficient methodology that facilitates selecting a polypeptide fragment that specifically inhibits the targeted protein-protein interaction has been established in this research. I have demonstrated an interaction domain, or a polypeptide responsible for the binding surface-formation, from either of the targeted proteins can be a competitive inhibitor against the interaction between the native proteins in vivo. A novel screening system with a quantitative reporter gene(an engineered fungal cDNA for secretory beta-galactosidase, LacA3) was also developed to facilitate a directed evolution of the inhibitor fragment.
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