Eiucidation of the mobilization mechanism of transposab leel ements

• Project/Area Number: 21770002
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: KAJIKAWA Masaki Tokyo Institute of Technology, 大学院・生命理研究科講師生命理工学研究科, 講師 (90361766)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 転移因子 / レトロトランスポゾン / 反復配列 / レトロポゾン

Research Abstract

Long interspersed elements (LINEs) are transposable elements that mobilize and amplify their own copies within eukaryotic genomes. LINEs mobilize via a mechanism called retrotransposition, in which transcribed LINE RNA is reverse transcribed into DNA that is then integrated into the host chromosome. Although the role of LINE-encoded proteins in retrotransposition has been revealed, the participation of host-encoded proteins has not been well investigated. Here, we present genetic evidence that the host-encoded proteins involved in repair of DNA double-strand breaks participate in LINE retrotransposition.

Research Products

• [Journal Article] Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition. (2009)
  • Author(s): Suzuki J., Yamaguchi K., Kajikawa M., Ichiyanagi K., Adachi N., Koyama H.,Takeda S., Okada N. (corresponding authors)
  • Journal Title: PLoS Genetics 5
  • URL: http://www.plosgenetics.org/
  • Peer Reviewed
• [Journal Article] Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition (2009)
  • Author(s): Jun Suzuki J., Yamaguchi K., Kajikawa M., Ichiyanagi K., Adachi N., Koyama H., Takeda S., Okadal N.(corresponding authors)
  • Journal Title: PLoS Genetics 5
  • Peer Reviewed
• [Presentation] Host factors are involved in the 5' joining of the LINE integration. (2010)
  • Author(s): Kajikawa M.Yamaguchi K., Okada N.
  • Organizer: ASM Conferences(mobile DNA).
  • Place of Presentation: Montreal, Canada.
  • Year and Date: 2010-04-28
• [Presentation] Host factors are involved in the 5' joining of the LINE integration (2010)
  • Author(s): Masaki Kajikawa, Katsumi Yamaguchi, Norihiro Okada
  • Organizer: ASM Conferences (mobile DNA)
  • Place of Presentation: モントリオール,カナダ
  • Year and Date: 2010-04-28