Functional analysis of Shu complex, a new mediator of Rad51 protein in recombination

• Project/Area Number: 21770005
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Osaka University
• Principal Investigator: SASANUMA Hiroyuki Osaka University, 蛋白質研究所, 特任研究員 (00531691)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 分子遺伝 / Rad51 / Dmc1 / 減数分裂 / 相同組換え / 出芽酵母 / 組換え

Research Abstract

Homologous recombination is essential mechanism to repair DNA damage, such as double strand breaks. In this grant period, I found that Shu complex assists the role of Rad51 protein, which plays a central role in homologous recombination. Based on research proposal, I also successfully determined the crystal structure of Shu complex, thereby could clarified the structural relationship between Rad51 and Shu protein complex.

Research Products

• [Journal Article] Rec8 Guides Canonical Spoll Distribution along Yeast Meiotic Chromosomes. (2009)
  • Author(s): Kugou, K., Fukuda, T., Yamada, S., Ito, M., Sasanuma, H., Mori, S., Katou, Y., Itoh, T., Matsumoto, K., Shibata, T., et al.
  • Journal Title: Mol Biol Cell 345 Pages: 1939-4586
• [Journal Article] タイトル『減数分裂期特異的DNA二重鎖切断機構』 (2009)
  • Author(s): 笹沼博之、太田邦史
  • Journal Title: 染色体サイクル(蛋白質核酸酵素・特集号) Pages: 459-465
• [Journal Article] Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination. (2008)
  • Author(s): Sasanuma, H., Hirota, K., Fukuda, T., Kakusho, N., Kugou, K., Kawasaki, Y., Shibata, T., Masai, H., Ohta, K.
  • Journal Title: Genes Dev 22 Pages: 398-410
• [Journal Article] Targeted induction of meiotic double-strand breaks reveals chromosomal domain-dependent regulation of Spo11 and interactions among potential sites of meiotic recombination. (2008)
  • Author(s): Fukuda, T., Kugou, K., Sasanuma, H., Shibata, T., Ohta, K.
  • Journal Title: Nucleic Acids Res 36 Pages: 984-997
• [Journal Article] Meiotic association between Spoll regulated by Rec102, Rec104 and Rec114. (2007)
  • Author(s): Shibata, T., Nicolas, A., Ohta, K.
  • Journal Title: Nucleic Acids Res, 35 Pages: 1119-1133
• [Journal Article] Mrell mediates gene regulation in yeast spore development. (2007)
  • Author(s): Kugou, K., Sasanuma, H., Matsumoto, K., Shirahige, K., Ohta, K.
  • Journal Title: Genes Genet Syst 82 Pages: 21-33
• [Presentation] Roles of histone modification enzymes, Set1 and Dot1, and the PAF transcription elongation complex during meiosis (2011)
  • Author(s): Miki Shinohara, Hiroyuki Sasanuma, Mohamad Bani, Akira Shinohara
  • Place of Presentation: Fukuoka, Japan
• [Presentation] PCSS complex facilitates the loading of Rad51 recombinase onto meiotic chromosomes (2010)
  • Author(s): Hiroyuki Sasanuma, Maki Tawaramoto, Miki Shinohara, Akira Shinohara
  • Organizer: 3R symposium
  • Place of Presentation: Toyama
• [Presentation] PCSS complex facilitates the loading of Rad51 recombinase onto meiotic chromosomes (2010)
  • Author(s): Hiroyuki Sasanuma, Maki Tawaramoto, Miki Shinohara, Akira Shinohara
  • Organizer: Epigenome Network, Development and Reprogramming of Germ Cells
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Rad51蛋白質の活性を制御する二つの因子PCSS複合体とSrs2蛋白質の解析 (2010)
  • Author(s): 笹沼博之、俵元麻貴、篠原美紀、篠原彰
  • Organizer: 核ダイナミクス研究会
  • Place of Presentation: 静岡
• [Presentation] Shu complex promotes the assembly of Rad51 on meiotic chromosomes (2010)
  • Author(s): 笹沼博之
  • Organizer: 3R symposium
  • Place of Presentation: 富山県国際会議場
• [Presentation] Shu complex promotes the assembly of Rad51 on meiotic chromosomes (2009)
  • Author(s): Hiroyuki Sasanuma, Maki Tawaramoto, Miki Shinohara, Akira Shinohara
  • Organizer: IUBMB
  • Place of Presentation: Shunghai, China
• [Presentation] 減数分裂期組換えに関与するShu蛋白質の機能解析 (2009)
  • Author(s): 笹沼博之、俵元麻貴、篠原美紀、篠原彰
  • Place of Presentation: 静岡
• [Presentation] Functional analysis of Shul/Shu2/Csm2/Psy3 complex in meiosis (2009)
  • Author(s): 笹沼博之
  • Organizer: IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 上海、中国
• [Presentation] PCSS複合体は、Rad51蛋白質の染色体上への結合を促進する (2009)
  • Author(s): 笹沼博之
  • Organizer: 3Rワークショップ2009
  • Place of Presentation: 滋賀、日本
• [Presentation] 減数分裂期組換えに関与するShu蛋白質の機能解析 (2009)
  • Author(s): 笹沼博之
  • Organizer: 核ダイナミクス研究会
  • Place of Presentation: 修善寺、静岡、日本
• [Presentation] 減数分裂期DNA鎖組換え反応に関与するShu蛋白質の機能解析 (2009)
  • Author(s): 笹沼博之
  • Organizer: 染色サイクル若手研究会
  • Place of Presentation: 御殿場、静岡、日本
• [Presentation] Shu complex promotes the assembly of Rad51 on meiotic chromosomes (2008)
  • Author(s): Hiroyuki Sasanuma, Miki Shinohara, Akira Shinohara
  • Place of Presentation: Shizuoka, Japan
• [Presentation] 減数分裂期におけるShu複合体の機能解析 (2008)
  • Author(s): 笹沼博之、三田理恵、俵元麻貴、篠原美紀、篠原彰
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: 北海道