Defining the modification mechanisms of DNA damage checkpoint in meiosis

• Project/Area Number: 21770006
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Osaka University
• Principal Investigator: USUI Takehiko Osaka University, 蛋白質研究所, 助教 (70533115)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: DNA二重鎖切断 / DNA損傷応答 / シグナル伝達 / ゲノム安定性 / 出芽酵母 / 相同染色体間組換え / 姉妹染色体間組換え / DNA二重鎖切断応答 / Rad53 / Rad9 / 細胞情報伝達

Research Abstract

In this study, Iasked why a DNA damage checkpoint kinase Rad53 fails to respond to meiotic programmed DNA double-strand breaks(DSBs).This was a mystery given that a single mitotic DSB can activate Rad53 and that about 200 programmed DSBs are formed in meiosis. I found that meiotic cells may suppress the mitotic types of recombination repair modulated by Rad53 to promote meiotic recombination. The data shed light on how cells alter mitotic systems to execute meiosis to produce normal gametes.

Research Products

• [Journal Article] Maintenance of the DNA damage checkpoint requires DNA damage-induced mediator protein oligomerization. (2009)
  • Author(s): Usui T., Foster S., Petrini J.H.J.
  • Journal Title: Molecular Cell 33 Pages: 147-159
• [Journal Article] Taking the time to make important decisions : the checkpoint effector kinases Chk1 and Chk2 and the DNA damage response. (2009)
  • Author(s): Stracker T.H., Usui T., Petrini J.H.J.
  • Journal Title: DNA Repair 8 Pages: 1047-1054
• [Journal Article] Taking the time to make important decisions : the checkpoint effector kinases Chkl and Chk2 and the DNA damage response. (2009)
  • Author(s): Stracker T.H., Usui T., Petrini J.H.J.
  • Journal Title: DNA Repair 8 Pages: 1047-1054
• [Presentation] 出芽酵母DNA損傷チェックポイントキナーゼRad53の減数分裂期における機能抑制の生理的意義 (2011)
  • Author(s): 臼井雄彦、篠原美紀、篠原彰
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 石川県山代温泉、ホテル瑠璃光
  • Year and Date: 2011-01-12
• [Presentation] Negative impacts on meiotic processes by activation of DNA damage checkpoint kinase Rad53 (2010)
  • Author(s): 臼井雄彦、篠原彰
  • Organizer: the budding yeast Chk2 ortholog第33回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市、神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Negative impacts on meiotic processes by activation of DNA damage checkpoint kinase Rad53, the budding yeast Chk2 ortholog (2010)
  • Author(s): 臼井雄彦、篠原彰
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市、神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Negative impacts of DNA damage checkpoint kinase activation on meiotic recombination (2010)
  • Author(s): 臼井雄彦、篠原彰
  • Organizer: 第7回3R国際シンポジウム
  • Place of Presentation: 富山県、富山国際会議場
  • Year and Date: 2010-10-27
• [Presentation] Negative impacts of DNA damage checkpoint kinase activation on meiotic recombination (2010)
  • Author(s): 臼井雄彦、篠原彰
  • Organizer: 第7回 3R国際シンポジウム
  • Place of Presentation: 富山県、富山国際会議場
  • Year and Date: 2010-10-27
• [Presentation] 出芽酵母DNA損傷チェックポイントキナーゼRad53の減数分裂期における機能抑制の生理的意義 (2010)
  • Author(s): 臼井雄彦、篠原彰
  • Organizer: 日本遺伝学会第82回大会ワークショップ「ゲノム安定性の維持に関わる細胞機構」
  • Place of Presentation: 北海道札幌、北海道大学高等教育機能開発総合センター
  • Year and Date: 2010-10-20
• [Presentation] 出芽酵母DNA損傷チェックポイントキナーゼRad53の減数分裂期における機能抑制の生理的意義 (2010)
  • Author(s): 臼井雄彦、篠原彰
  • Organizer: 日本遺伝学会第82回大会ワークショップ「ゲノム安定性の維持に関わる細胞機構」
  • Place of Presentation: 北海道札幌、北海道大学高等教育機能開発総合センター(招待講演)
  • Year and Date: 2010-10-20
• [Presentation] DNA損傷チェックポイントキナーゼChk2の出芽酵母ホモログRad53の減数分裂期における機能抑制 (2010)
  • Author(s): 臼井雄彦、篠原彰
  • Organizer: 生殖サイクル若手勉強会2010
  • Place of Presentation: 茨城県筑波、つくばグランドホテル
  • Year and Date: 2010-07-22
• [Presentation] 出芽酵母DNA損傷チェックポイント蛋白Rad9とRad53の減数分裂期における抑制機構と生理的意義 (2009)
  • Author(s): 臼井雄彦、篠原彰
  • Organizer: 第20回DNA複製、組換え、修復ワークショップ
  • Place of Presentation: 琵琶湖コンファレンスセンター(滋賀県)
  • Year and Date: 2009-11-01