Study of Circadian Clock protein homolog in Prochlorococcus

• Project/Area Number: 21770008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Yokohama City University
• Principal Investigator: KUTUNA Sinnsuke Yokohama City University, 生命ナノシステム科学研究科, 准教授 (30315824)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: 概日時計 / タンパク質 / 藍藻 / プロクロロコッカス / KaiC / KaiB

Research Abstract

KaiC protein was detected in Prochlorococcus cell. Transformation of the cell with E. coli conjugation was not established. Therefore, a knock-down method as inhibition of translation technique was applied. Natural oligonucleotide had a week effect on Prochlorococcus KaiC. Now artificial oligonucleotide LNA is being examined.