Construction of system and analysis of related factors for de novo centromere formation in fission yeast

• Project/Area Number: 21770009
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Kurume University
• Principal Investigator: SATO Hiroshi Kurume University, 分子生命科学研究所, 助教 (00421313)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: セントロメア / 染色体分 / 裂酵母 / 染色体 / 分裂酵母

Research Abstract

Artificial fused chromosome containing two centromeres were converted into a stable monocentric chromosome by functionally inactivating one centromere in fission yeast. We developed a method to generate de novo centromere from the fused chromosome on which the active centromere was deleted by site-directed recombination and only inactive centromere was contained. The analysis using this system in mutant strains indicated that the heterochromatin and deacetylation of histone prevent revival of the inactivated centromere.

Research Products

• [Presentation] Epigenetic centromere inactivation consists of two steps : kinetochore disassembly and subsequent heterochromatinization (2011)
  • Author(s): 佐藤浩
  • Organizer: International Symposium on the Physicochemical Field for Genetic Activities(GENOFIEL~D「~2011)
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2011-01-25
• [Presentation] Epigenetic centromere inactivation consists of two steps : kinetochore disassembly and subsequent heterochromatinization (2011)
  • Author(s): 佐藤浩
  • Organizer: International Symposium on the Physicochemical Field for Genetic Activities (GENOFIELD2011)
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2011-01-25
• [Presentation] ヘテロクロマチンによる不活性化セントロメアの再活性化抑制 (2011)
  • Author(s): 佐藤浩
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 石川県
  • Year and Date: 2011-01-12
• [Presentation] ヘテロクロマチンによる不活性化セントロメアの再活性化抑制 (2011)
  • Author(s): 佐藤浩
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 山代温泉 瑠璃光(石川県)
  • Year and Date: 2011-01-12
• [Presentation] Heterochromatin suppresses kinetochore reassembly on epigenetically inactivated centromeres (2010)
  • Author(s): 佐藤浩
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Heterochromatin suppresses kinetochore reassembly on epigenetically inactivated centromeres (2010)
  • Author(s): 佐藤浩
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-07
• [Presentation] 染色体構造改変によるセントロメア制御機構の解析 (2010)
  • Author(s): 佐藤浩
  • Organizer: 第28回YEAST WORKSHOP
  • Place of Presentation: 福山
  • Year and Date: 2010-11-12
• [Presentation] EPIGENETIC AND GENETIC CENTROMERE INACTIVATION IN A DICENTRIC CHROMOSOME (2009)
  • Author(s): Hiroshi Sato
  • Organizer: The 5th International Fission Yeast Meeting(Pombe 2009)
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京都)
  • Year and Date: 2009-10-28
• [Presentation] Epigenetic and genetic centromere inactivation in a dicentric chromosome (2009)
  • Author(s): 佐藤浩
  • Organizer: The Fifth International Fission Yeast Meeting
  • Place of Presentation: 東京