Construction of system and analysis of related factors for de novo centromere formation in fission yeast
Project/Area Number |
21770009
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Genetics/Genome dynamics
|
Research Institution | Kurume University |
Principal Investigator |
SATO Hiroshi Kurume University, 分子生命科学研究所, 助教 (00421313)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | セントロメア / 染色体分 / 裂酵母 / 染色体 / 分裂酵母 |
Research Abstract |
Artificial fused chromosome containing two centromeres were converted into a stable monocentric chromosome by functionally inactivating one centromere in fission yeast. We developed a method to generate de novo centromere from the fused chromosome on which the active centromere was deleted by site-directed recombination and only inactive centromere was contained. The analysis using this system in mutant strains indicated that the heterochromatin and deacetylation of histone prevent revival of the inactivated centromere.
|
Report
(3 results)
Research Products
(9 results)