Elucidation of ICE1-dependent cold signaling network
| Project/Area Number |
21770032
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
MIURA Kenji 筑波大学, 生命環境系, 助教 (00507949)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 低温シグナル伝達 / ICE1の安定化 / カルモジュリン様タンパク質 / 転写因子ICE1 / カルシウムシグナル / 低温誘導性遺伝子 / 低温応答 / カルシウムチャネル / タンパク質の安定性 / ユビキチン / 低温応答性遺伝子 / MYC型転写因子 / 翻訳後修飾因子SUMO / サリチル酸 / ユピキチン |
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| Research Abstract |
To identify the transactivation system of ICE1, which is a regulator of cold signaling, site-directed mutagenesis was introduced. Substitution of serine 403 to alanine inhibited degradation of ICE1, consequently, ICE1(S403A) was stabilized. This stabilization leads to improving cold tolerance. And to understand the regulatory mechanisms of ICE1, interactors of ICE1 were isolated by yeast 2-hybrid screening. MYC-type transcription factors, kinases, and calmodulin-like proteins were identified as ICE1-interactors. Furthermore, the results suggested that calmodulin-like proteins are involved in regulation of cold signaling and cold tolerance.
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Report
(4 results)
Research Products
(32 results)
