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Elucidation of ICE1-dependent cold signaling network

Research Project

Project/Area Number 21770032
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Tsukuba

Principal Investigator

MIURA Kenji  筑波大学, 生命環境系, 助教 (00507949)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords低温シグナル伝達 / ICE1の安定化 / カルモジュリン様タンパク質 / 転写因子ICE1 / カルシウムシグナル / 低温誘導性遺伝子 / 低温応答 / カルシウムチャネル / タンパク質の安定性 / ユビキチン / 低温応答性遺伝子 / MYC型転写因子 / 翻訳後修飾因子SUMO / サリチル酸 / ユピキチン
Research Abstract

To identify the transactivation system of ICE1, which is a regulator of cold signaling, site-directed mutagenesis was introduced. Substitution of serine 403 to alanine inhibited degradation of ICE1, consequently, ICE1(S403A) was stabilized. This stabilization leads to improving cold tolerance. And to understand the regulatory mechanisms of ICE1, interactors of ICE1 were isolated by yeast 2-hybrid screening. MYC-type transcription factors, kinases, and calmodulin-like proteins were identified as ICE1-interactors. Furthermore, the results suggested that calmodulin-like proteins are involved in regulation of cold signaling and cold tolerance.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (32 results)

All 2012 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 6 results) Presentation (13 results) Remarks (8 results)

  • [Journal Article] ICE1 Ser403 is necessary for protein stabilization and regulation of cold signaling and tolerance2011

    • Author(s)
      Miura K, 他4名
    • Journal Title

      Plant J

      Volume: 67 Pages: 269-279

    • NAID

      120007137312

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Root architecture remodeling induced by phosphate starvation2011

    • Author(s)
      Sato A, Miura K
    • Journal Title

      Plant Signal. Behav

      Volume: 6 Pages: 1122-1126

    • Related Report
      2011 Final Research Report
  • [Journal Article] ICE1 Ser403 is necessary for protein stabilization and regulation of cold signaling and tolerance2011

    • Author(s)
      Miura K, et al.
    • Journal Title

      Plant J.

      Volume: 67 Issue: 2 Pages: 269-279

    • DOI

      10.1111/j.1365-313x.2011.04589.x

    • NAID

      120007137312

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Root architecture remodeling induced by phosphate starvation2011

    • Author(s)
      Sato A, Miura K
    • Journal Title

      Plant Signal. Behav.

      Volume: 6 Issue: 8 Pages: 1122-1126

    • DOI

      10.4161/psb.6.8.15752

    • Related Report
      2011 Annual Research Report
  • [Journal Article] SIZ1 controls cell growth and plant development in Arabidopsis through salicylic acid2010

    • Author(s)
      Miura K, Lee J, Miura T, Hasegawa PM
    • Journal Title

      Plant Cell Physiol

      Volume: 51 Pages: 103-113

    • NAID

      120007137826

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] SIZ1, a small ubiquitin-related modifier ligase, controls cold signaling through regulation of salicylic acid accumulation2010

    • Author(s)
      Miura K, Ohta M
    • Journal Title

      J. Plant Physiol

      Volume: 167 Pages: 555-560

    • NAID

      120007138138

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cold-responsive gene regulation during cold acclimation in plants2010

    • Author(s)
      Lissarre M, Ohta M, Sato A, Miura K
    • Journal Title

      Plant Signal. Behav

      Volume: 5 Pages: 948-952

    • Related Report
      2011 Final Research Report
  • [Journal Article] Sumoylation and other ubiquitin-like post-translational modifications in plants2010

    • Author(s)
      Miura K, Hasegawa PM
    • Journal Title

      Trends Cell Biol

      Volume: 20 Pages: 223-232

    • Related Report
      2011 Final Research Report
  • [Journal Article] SIZ1, a small ubiqutin-related modifier ligase, controls cold signaling through regulation of salicylic acid accumulation2010

    • Author(s)
      Miura K, Ohta M
    • Journal Title

      J.Plant Physiol. (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SIZ1 controls cell growth and plant development in Arabiodpsis through salicylic acid2010

    • Author(s)
      Miura K, et al.
    • Journal Title

      Plant Cell Physiol 51

      Pages: 103-113

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sumoylation and other ubiquitin-like post-translational modifications in plants2010

    • Author(s)
      Miura K, Hasegawa PM
    • Journal Title

      Trends Cell Biol. (In press)

    • Related Report
      2009 Annual Research Report
  • [Presentation] Regulatory mechanisms of ICE1 for cold signaling and tolerance2012

    • Author(s)
      三浦謙治
    • Organizer
      The 1st International Symposium on Plant Environmental Sensing
    • Place of Presentation
      東大寺総合文化センター(奈良)
    • Year and Date
      2012-03-20
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Three MYC-type transcription factors, interactors of ICE1, negatively regulate cold signaling and tolerance2011

    • Author(s)
      佐藤愛子、太田賢、Jian-Kang Zhu、Paul M.Hasegawa、三浦謙治
    • Organizer
      日本農芸化学会2011年大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] SIZ1におけるPHDドメインの役割及びSIZ1とサリチル酸の関連性2010

    • Author(s)
      三浦謙治
    • Organizer
      第8回SUMO研究会
    • Place of Presentation
      筑波大学(つくば)
    • Year and Date
      2010-11-26
    • Related Report
      2011 Final Research Report
  • [Presentation] SIZ1におけるPHDドメインの役割及びSIZ1とサリチル酸の関連性2010

    • Author(s)
      三浦謙治
    • Organizer
      第8回SUMO研究会
    • Place of Presentation
      筑波大学
    • Year and Date
      2010-11-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Hasegawa Enhancement of ICE1 activity for cold tolerance by substitution of serine 403, which regulates ubiquitylation of ICE12010

    • Author(s)
      三浦謙治、太田賢、Paul M
    • Organizer
      21th International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2010-06-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Enhancement of ICE1 activity for cold tolerance by substitution of serine 403, which regulates ubiquitylation of ICE12010

    • Author(s)
      三浦謙治、太田賢、Hasegawa PM
    • Organizer
      21^<th> International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-06-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] SUMO E3 ligase high ploidy2 regulates endocycle onset and meristem maintenance in Arabidopsis.2010

    • Author(s)
      石田喬志、藤原すみれ、三浦謙治、Stacey N、吉村美香、Schneider K、足立澄子、南澤和則、梅田正明、杉本慶子
    • Organizer
      21^<th> International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-06-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] ICE1活性化による凍結耐性の向上2010

    • Author(s)
      三浦謙治、太田賢、Paul M. Hasegawa
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2010-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] ICE1活性化による凍結耐性の向上2010

    • Author(s)
      三浦謙治、太田賢、Hasegawa PM
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] SIZ1はサリチル酸蓄積の調節を行って細胞及び植物の成長を制御する2010

    • Author(s)
      三浦謙治、Lee J、三浦友子、Hasegawa PM
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] ICE1と相互作用するMYC67,70,71は低温応答の負の制御因子である2010

    • Author(s)
      太田賢、Jian-Kang Zhu、Paul M. Hasegawa、三浦謙治
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本大学(熊本)
    • Year and Date
      2010-03-18
    • Related Report
      2011 Final Research Report
  • [Presentation] ICE1と相互作用するMYC67, 70, 71は低温応答の負の制御因子である2010

    • Author(s)
      太田賢、Zhu JK、Hasegawa PM、三浦謙治
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物SUMO E_3 ligase SIZ1によるアブシジン酸シグナル伝達機構の調節およびSIZ1内に存在するPHDドメインの役割2009

    • Author(s)
      三蒲謙治
    • Organizer
      第7回SUMO研究会
    • Place of Presentation
      関西学院大学
    • Year and Date
      2009-11-22
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.gene.tsukuba.ac.jp/~kmiura/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://wakate.biol.tsukuba.ac.jp

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.gene.tsukuba.ac.jp/~kmiura/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://wakate.biol.tsukuba.ac.jp

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.gene.tsukuba.ac.jp/~kmiura/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://wakate.biol.tsukuba.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.gene.tsukuba.ac.jp/~kmiura/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://wakate.biol.tsukuba.ac.jp/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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