Chromatin immunoprecipitation-based analysis of transcriptional regulation in Arabidopsis thaliana chloroplasts
| Project/Area Number |
21770034
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Chiba University |
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Principal Investigator |
HANAOKA Mitsumasa Chiba University, 大学院・園芸学研究科, 特任准教授 (30508122)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 葉緑体 / 転写制御 / ChIP / 環境応答 / シロイヌナズナ / 色素体分化 / シグマ因子 / 転写因子 |
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| Research Abstract |
In this study, to further clarify transcriptional regulation in Arabidopsis thaliana chloroplasts, Chromatin immunoprecipitation (ChIP)-based method has been established. Novel target promoters for SIG1, an essential sigma factor in A.thaliana, as well as those for SIG5, a multiple stress-induced chloroplast sigma factor were identified respectively. These results suggest that ChIP analysis is useful to understand transcriptional regulation of chloroplast genes.
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Report
(3 results)
Research Products
(59 results)
