Functional analysis of the PsbP homologs in response to high light stress in plants.

• Project/Area Number: 21770043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: IFUKU Kentaro Kyoto University, 生命科学研究科, 助教 (50359783)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 植物 / 光合成 / 環境ストレス応答 / 光化学系II / 酸素発生系 / 強光ストレス応答 / 光化学系 / シロイヌナズナ

Research Abstract

Higher plants have a number of PsbP homologs (PsbP-like proteins : PPLs, PsbP-domain proteins : PPDs) in addition to the authentic PsbP, which regulates the oxygen-evolving reaction in photosystem II (PSII). Among the PsbP homologs, PPL1 is co-expressed with PPD3 and required for the repair of photo-damaged PSII. Further analyses suggest that PPD3 is dispensable for high-light stress tolerance, while PPL1 has an important role in response to high-light stress in plants.

Research Products

• [Journal Article] High and low potential forms of the Q_A quinone electron acceptor in Photosystem II of Thermosynechococcus elongatus and spinach. (2011)
  • Author(s): Ido K, Gross CM, Guerrero F, Sedoud A, Lai TL, Ifuku K, Rutherford AW, Krieger-Liszkay A
  • Journal Title: J.Photochem. Photobiol.B. (in press.)
  • Peer Reviewed
• [Journal Article] Molecular functions of PsbP and PsbQ proteins in the photosystem II supercomplex. (2011)
  • Author(s): Ifuku K, Ido K, Sato F
  • Journal Title: J.Photochem. Photobiol. B (in press.)
  • Peer Reviewed
• [Journal Article] Molecular functions of PsbP and PsbQ proteins in the photosystem II supercomplex (2011)
  • Author(s): Ifuku K, Ido K, Sato F
  • Journal Title: J.Photochem.Photobiol.B Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] High and low potential forms of the Q(A) quinone electron acceptor in Photosystem II of Thermosynechococcus elongatus and spinach. (2011)
  • Author(s): Ido K, Gross CM, Guerrero F, Sedoud A, Lai TL, Ifuku K, William Rutherford A, Krieger-Liszkay A.
  • Journal Title: J.Photochem.Photobiol.B Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Functional Analysis of PsbR in PsbP Binding to Photosystem II (2011)
  • Author(s): Ido K, Ifuku K, Sato F
  • Journal Title: Proceedings of the 15^<th> International Congress on Photosynthesis Volume: (印刷中)
• [Journal Article] Functional analysis of PsbP-like protein 1 (PPL1) in Arabidopsis (2011)
  • Author(s): Matsui S, Ishihara S, Ido K, Ifuku K, Sato F
  • Journal Title: Proceedings of the 15^<th> International Congress on Photosynthesis Volume: (印刷中)
• [Journal Article] Functional roles of the amino- and carboxyl-regions of PsbP protein in photosystem II (2011)
  • Author(s): Kakiuchi S, Ifuku K, Tomita M, Ido K, Noguchi T, Sato F
  • Journal Title: Proceedings of the 15^<th> International Congress on Photosynthesis Volume: (印刷中)
• [Journal Article] Molecular functions of oxygen-evolving complex family proteins in photosynthetic electron flow. (2010)
  • Author(s): Ifuku K, Ishihara S, Sato F
  • Journal Title: J.Integr.Plant Biol. 52 Pages: 723-734
  • Peer Reviewed
• [Journal Article] Three PsbQ-like proteins are reqired for the function of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis. (2010)
  • Author(s): Yabuta S, Ifuku K, Takabayashi, A Ishihara S, Ido K, Ishikawa N, Endo T, Sato F
  • Journal Title: Plant Cell Physiol. 51 Pages: 866-876
  • Peer Reviewed
• [Journal Article] Molecular functions of oxygen-evolving complex family proteins in photosynthetic electron flow (2010)
  • Author(s): Ifuku K, Ishihara S, Sato F.
  • Journal Title: J.Integr.Plant Biol. Volume: 52 Pages: 723-734
  • Peer Reviewed
• [Journal Article] Three PsbQ-like proteins are required for the function of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis (2010)
  • Author(s): Yabuta S, Ifuku K, Takabayashi A, Ishihara S, Ido K, Ishikawa N, Endo T, Sato F
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 866-876
  • NAID: 10027349591
  • Peer Reviewed
• [Journal Article] FTIR evidence that the PsbP extrinsic protein induces protein conformational changes around the oxygen-evolving Mn cluster in photosystem II. (2009)
  • Author(s): Tomita M, Ifuku K, Sato F, Noguchi T
  • Journal Title: Biochemistry 48 Pages: 6318-6325
  • Peer Reviewed
• [Journal Article] The variegated mutants lacking chloroplastic FtsHs are defective in D1 degradation and accumulate reactive oxygen species. (2009)
  • Author(s): Kato Y, Miura E, Ido K, Ifuku K, Sakamoto W
  • Journal Title: Plant Physiol. 151 Pages: 1790-1801
  • Peer Reviewed
• [Journal Article] The variegated mutants lackingchloroplastic FtsHs are defective in D1 degradation and accumulate reactiveoxygen species. (2009)
  • Author(s): Kato Y, Miura E, Ido K, Ifuku K, Sakamoto W.
  • Journal Title: Plant Physiology 151 Pages: 1790-1801
  • Peer Reviewed
• [Journal Article] FTIR evidence that the PsbP extrinsic protein induces protein conformational changes around the oxygen-evolving Mn cluster in photosystem II. (2009)
  • Author(s): Tomita M, Ifuku K, Sato F, Noguchi T.
  • Journal Title: Biochemistry 48 Pages: 6318-6325
  • Peer Reviewed
• [Presentation] PsbPが光化学系II Q_Aの酸化還元電位に及ぼす影響の解析 (2011)
  • Author(s): 井戸邦夫, 伊福健太郎, Anja Krieger-Liszkay, 佐藤文彦
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-27
• [Presentation] PsbPが光化学系II QAの酸化還元電位に及ぼす影響の解析 (2011)
  • Author(s): 井戸邦夫, 伊福健太郎, Anja Krieger-Liszkay, 佐藤文彦
  • Organizer: 日本農芸化学会 2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-27
• [Presentation] シロイヌナズナPsbP-Like1(PPL1)タンパク質の分子機能解析 (2011)
  • Author(s): 松井信太郎, 石原靖子, 伊福健太郎, 佐藤文彦
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-21
• [Presentation] シロイヌナズナPsbP-Like 1(PPL1)タンパク質の分子機能解析 (2011)
  • Author(s): 松井信太郎, 石原靖子, 伊福健太郎, 佐藤文彦
  • Organizer: 第52回 日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-21
• [Presentation] 高等植物における光化学系II酸素発生系タンパク質の進化と機能 (2010)
  • Author(s): 伊福健太郎
  • Organizer: 光合成シンポジウム「光合成による光エネルギー利用の過去・現状・未来」
  • Place of Presentation: 京都大学
  • Year and Date: 2010-12-29
• [Presentation] Molecular function of PsbP-like protein 1 (PPL1) in Arabidopsis (2010)
  • Author(s): Matsui S, Ishihara S, Ido K, Ifuku K, Sato F
  • Organizer: APRU Research Symposium-Interface between Molecular Biology and Nano-Biology-
  • Place of Presentation: Kyoto
  • Year and Date: 2010-11-25
• [Presentation] 光化学系II酸素発生系タンパク質の分子進化と植物の環境適応 (2010)
  • Author(s): 松井信太郎, 石原靖子, 伊福健太郎, 佐藤文彦
  • Organizer: 2010年度日本農芸化学会関西支部大会
  • Place of Presentation: 近畿大学農学部
  • Year and Date: 2010-10-03
• [Presentation] Functional analysis of PsbP-like protein 1 (PPL1) in Arabidopsis (2010)
  • Author(s): Matsui S, Ishihara S, Ido K, Ifuku K, Sato F
  • Organizer: 15th International Congress on Photosynthesis
  • Place of Presentation: 北京
  • Year and Date: 2010-08-26
• [Presentation] Functional Analysis of PsbR in PsbP Binding to Photosystem II (2010)
  • Author(s): Ido K, Ifuku K, Sato F
  • Organizer: 15th International Congress on Photosynthesis,
  • Place of Presentation: 北京
  • Year and Date: 2010-08-26
• [Presentation] Functional analysis of PsbP-like protein 1 (PPL1) in Arabidopsis (2010)
  • Author(s): Matsui S, Ishihara S, Ido K, Ifuku K, Sato F
  • Organizer: 15^<th> International Congress on Photosynthesis
  • Place of Presentation: 北京 中国
  • Year and Date: 2010-08-26
• [Presentation] Functional Analysis of PsbR in PsbP Binding to Photosystem II (2010)
  • Author(s): Ido K, Ifuku K, Sato F
  • Organizer: 15^<th> International Congress on Photosynthesis
  • Place of Presentation: 北京 中国
  • Year and Date: 2010-08-26
• [Presentation] Molecular functions of oxygen-evolving complex family proteins in linear and cyclic photosynthetic electron flows (2010)
  • Author(s): Ifuku K, 他8名
  • Organizer: 15th International Congress on Photosynthesis
  • Place of Presentation: 北京
  • Year and Date: 2010-08-24
• [Presentation] Functional roles of the amino- and carboxyl-regions of PsbP protein in photosystem II (2010)
  • Author(s): Kakiuchi S, Tomita M, Ido K, Ifuku K, Noguchi T, Sato F
  • Organizer: 15th International Congress on Photosynthesis
  • Place of Presentation: 北京
  • Year and Date: 2010-08-24
• [Presentation] Molecular functions of oxygen-evolving complex family proteins in linear and cyclic photosynthetic electron flows (2010)
  • Author(s): Ifuku K, Ishihara S, Takabayashi S, Yabuta S, Ido K, Ishikawa N, Matsui S, Endo T, Sato F
  • Organizer: 15^<th> International Congress on Photosynthesis
  • Place of Presentation: 北京 中国
  • Year and Date: 2010-08-24
• [Presentation] Functional roles of the amino- and carboxyl-regions of PsbP protein in photosystem II (2010)
  • Author(s): Kakiuchi S, Tomita M, Ido K, Ifuku K, Noguchi T, Sato F
  • Organizer: 15^<th> International Congress on Photosynthesis
  • Place of Presentation: 北京 中国
  • Year and Date: 2010-08-24
• [Presentation] Molecular functions of OEC family proteins in linear and cyaclic photothynthetic electron flow (2010)
  • Author(s): Ifuku K
  • Organizer: French photosynthesis meeting
  • Place of Presentation: Paris
  • Year and Date: 2010-05-07
• [Presentation] Molecular functions of OEC family proteins in linear and cyclic photosynthetic electron flow (2010)
  • Author(s): Ifuku K
  • Organizer: French photosynthesis meeting
  • Place of Presentation: Paris, France(招待講演)
  • Year and Date: 2010-05-07
• [Presentation] 植物の環境応答におけるOECホモログタンパク質の多様な機能 (2010)
  • Author(s): 伊福健太郎, 他7名
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: Paris
  • Year and Date: 2010-03-28
• [Presentation] 植物の環境応答におけるOECホモログタンパク質の多様な機能 (2010)
  • Author(s): 伊福健太郎, 他7名
  • Organizer: 日本農芸化学会 201年度大会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-28
• [Presentation] 高等植物PsbPタンパク質のC末端側ドメイン機能の解析 (2010)
  • Author(s): 垣内秀介、富田めぐみ、井戸邦夫、伊福健太郎、野口巧、佐藤文彦
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Presentation] 葉緑体NAD(P)H dehydrogenase複合体機能には複数のPsbQ-Like(PQL)タンパク質が関与する (2010)
  • Author(s): 薮田真也、石原靖子、高林厚史、井戸邦夫、遠藤剛、伊福健太郎、佐藤文彦
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-20
• [Presentation] シロイヌナズナPsbPホモログを介した強光ストレス応答機構の解析 (2010)
  • Author(s): 松井信太郎、石原靖子、井戸邦夫、伊福健太郎、佐藤文彦
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-18
• [Presentation] OEC protein family : thylakoid lumenal factors regulating the photosynthetic electron transport」 (2009)
  • Author(s): 伊福健太郎
  • Organizer: NAISTグローバルCOEワークショップ「植物の環境応答」
  • Place of Presentation: 奈良
  • Year and Date: 2009-03-04
• [Presentation] Molecular functions of OEC protein family regulating photosynthetic electron transport chain (2009)
  • Author(s): Kentaro Ifuku
  • Organizer: the 5^<th> Germany-Japan Binational Seminar
  • Place of Presentation: 筑波
• [Remarks] ホームページ等
  • URL: http://www.lif.kyoto-u.ac.jp/labs/callus/
• [Remarks]
  • URL: http://www.lif.kyoto-u.ac.jp/labs/callus/
• [Remarks]
  • URL: http://www.lif.kyoto-u.ac.jp/labs/callus/