Functional analysis of protein phosphatase 1 in stomatal guard cells

• Project/Area Number: 21770051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyushu University
• Principal Investigator: TAKEMIYA Atsushi Kyushu University, 大学院理学研究院, 助教 (80448406)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 環境応答 / 気孔 / 青色光 / プロテインホスファターゼ / プロテインホスファターゼ1 / フォトトロピン / H^+-ATPase

Research Abstract

Stomata in the epidermis of aerial plants open in response to blue light, which facilitates gas exchange between leaves and atmosphere, allowing the uptake of CO_2 for photosynthesis and the loss of water via transpiration. In this study, we investigated the molecular mechanism of blue light signaling mediated by protein phosphatase 1 (PP1) in guard cells, and identified the regulatory subunit and putative substrate proteins of PP1 in this process.

Research Products

• [Journal Article] Phosphatidic acid inhibits blue light-induced stomatal opening via inhibition of protein phosphatase 1 (2010)
  • Author(s): Atsushi Takemiya, Ken-ichiro Shimazaki
  • Journal Title: Plant Physiol. 153 Pages: 1555-1562
  • Peer Reviewed
• [Journal Article] Phototropin signaling and stomatal opening as a model case (2010)
  • Author(s): Shin-ichiro Inoue, Atsushi Takemiya, Ken-ichiro Shimazaki
  • Journal Title: Current Opinion in Plant Biology 13 Pages: 587-593
  • Peer Reviewed
• [Journal Article] Biochemical characterization of in vitro phosphorylation and dephosphorylation of the plasma membrane H+-ATPase (2010)
  • Author(s): Yuki Hayashi, Suguru Nakamura, Atsushi Takemiya, Yohei Takahashi, Ken-ichiro Shimazaki, Toshinori Kinoshita
  • Journal Title: Plant & Cell Physiology 51 Pages: 1186-1196
  • NAID: 10027350906
  • Peer Reviewed
• [Journal Article] Phosphatidic acid inhibits blue light-induced stomatal opening via inhibition of protein phosphatase 1 (2010)
  • Author(s): Takemiya, Shimazaki
  • Journal Title: Plant Physiology Volume: 153 Pages: 1555-1562
  • Peer Reviewed
• [Journal Article] Biochemical characterization of in vitro phosphorylation and dephosphorylation of the plasma membrane H^+-ATPase (2010)
  • Author(s): Hayashi, et al
  • Journal Title: Plant & Cell Physiology Volume: 51 Pages: 1186-1196
  • Peer Reviewed
• [Journal Article] Phototropin signaling and stomatal opening as a model case (2010)
  • Author(s): Inoue, et al
  • Journal Title: Current Opinion in Plant Biology Volume: 13 Pages: 587-593
  • Peer Reviewed
• [Journal Article] Identification and Functional Characterization of Inhibitor-3, a Regulato ry Subunit of Protein Phosphatase 1 in Plants (2009)
  • Author(s): Atsushi Takemiya, Chie Ariyoshi, Ken-ichiro Shimazaki
  • Journal Title: Plant Physiol. 150 Pages: 144-156
  • Peer Reviewed
• [Journal Article] 青色光受容体フォトトロピンに依存した植物の生長制御 (2009)
  • Author(s): 井上晋一郎、武宮淳史、島崎研一郎
  • Journal Title: 光合成研究 19 Pages: 4-8
  • NAID: 40017303834
• [Journal Article] Identification and Functional Characterization of Inhibitor-3, a Regulatory Subunit of Protein Phosphatase 1 in Plants. (2009)
  • Author(s): Takemiya, A., et al
  • Journal Title: Plant Physiology 150 Pages: 144-156
  • Peer Reviewed
• [Presentation] PP1調節サブユニットPRS2は気孔の青色光情報伝達を制御する (2011)
  • Author(s): 武宮淳史、矢野貴之、山内翔太、有吉千絵、島崎研一郎
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北大学
• [Presentation] 2D-DIGE(Two-dimensional difference gelelectrophoresis)を用いた孔辺細胞青色光情報伝達におけるリン酸化タンパク質の探索 (2011)
  • Author(s): 矢野貴之、武宮淳史、島崎研一郎
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北大学
• [Presentation] シロイヌナズナ孔辺細胞青色光情報伝達におけるRPT2の機能解析 (2011)
  • Author(s): 堤俊文、武宮淳史、原田明子、島崎研一郎
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北大学
• [Presentation] PP1調節サブユニットPRS2は気孔の青色光情報伝達を制御する (2011)
  • Author(s): 武宮淳史、矢野貴之、山内翔太、有吉千絵、島崎研一郎
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] 2D-DIGE(Two-dimensional difference gel electrophoresis)を用いた孔辺細胞青色光情報伝達におけるリン酸化タンパク質の探索 (2011)
  • Author(s): 矢野貴之、武宮淳史、島崎研一郎
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] シロイヌナズナ孔辺細胞青色光情報伝達におけるRPT2の機能解析 (2011)
  • Author(s): 堤俊文、武宮淳史、原田明子、島崎研一郎
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北大学(宮城県)
• [Presentation] 気孔の青色光シグナル伝達の分子機構 (2010)
  • Author(s): 武宮淳史
  • Organizer: 第486回三学会合同福岡例会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-12-04
• [Presentation] 気孔の青色光シグナル伝達の分子機構 (2010)
  • Author(s): 武宮淳史
  • Organizer: 第486回三学会合同福岡例会
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2010-12-04
• [Presentation] Protein phosphatase 1 regulates stomatal movements by integrating ABA and blue light signaling in guard cells (2010)
  • Author(s): Atsushi Takemiya, Yohei Takahashi, Ken-ichiro Shimazaki
  • Organizer: 20^th International Conference on Plant Growth Substances
  • Place of Presentation: Taragona, Spain
• [Presentation] 孔辺細胞青色光情報伝達におけるRPT2の機能解析 (2010)
  • Author(s): 堤俊文、武宮淳史、原田明子、島崎研一郎
  • Organizer: 第60回日本植物学会九州支部会
  • Place of Presentation: 九州大学
• [Presentation] Inhibitor-3はPP1調節サブユニットとしてシロイヌナズナの初期胚発生に機能する (2010)
  • Author(s): 武宮淳史、有吉千絵、島崎研一郎
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
• [Presentation] 気孔孔辺細胞における青色光シグナルのイオン輸送への変換 (2010)
  • Author(s): 島崎研一郎、井上晋一郎、武宮淳史
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
• [Presentation] 青色光受容体フォトトロピンの"Flippase-kinase"としての役割の解明 (2010)
  • Author(s): 相原悠介、山本隆晴、武宮淳史、鈴木友美、加藤詩子、梅田真郷、田中一馬、島崎研一郎、長谷あき, ら
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
• [Presentation] Protein phosphatase 1 regulates stomatal movements by integrating ABA and blue light signaling in guard cells (2010)
  • Author(s): Takemiya, A., Takahashi, Y., Shimazaki, K.
  • Organizer: 20th International Conference on Plant Growth Substances
  • Place of Presentation: Taragona, Spain
• [Presentation] 孔辺細胞青色光情報伝達におけるRPT2の機能解析 (2010)
  • Author(s): 堤俊文、武宮淳史、原田明子、島崎研一郎
  • Organizer: 第60回日本植物学会九州支部会
  • Place of Presentation: 九州大学(福岡県)
• [Presentation] Protein phosphatase 1 is a central regulator of blue light-dependent stomatal o pening mediated by phototropins (2009)
  • Author(s): Atsushi Takemiya, Ken-ichiro Shimazaki
  • Organizer: Memorial Symposium for the 25th International Prize for Biology" Biology of Sensing"
  • Place of Presentation: 京都大学
• [Presentation] 気孔孔辺細胞において青色光により活性化される細胞膜H+-ATPaseのリン酸化反応の解析 (2009)
  • Author(s): 中村英、武宮淳史、島崎研一郎、木下俊則
  • Organizer: 第15回日本光生物学協会年会
  • Place of Presentation: 自然科学研究機構岡崎コンフェレンスセンター
• [Presentation] Type 1 protein phosphatase is a crosstalk point between phototropin and abscisic acid signaling in guard cells (2009)
  • Author(s): Ken-ichiro Shimazaki, Atsushi Takemiya
  • Organizer: 15^th International Congress on Photobiology
  • Place of Presentation: Dusseldorf, Germany
• [Presentation] Inhibition of blue light-dependent stomatal opening by phosphatidic acid through abscisic acid signaling pathway in guard cells (2009)
  • Author(s): Atsushi Takemiya, Ken-ichiro Shimazaki
  • Organizer: 15^th International Congress on Photobiology
  • Place of Presentation: Dusseldorf, Germany
• [Presentation] Inhibition of blue light-dependent stomatal opening by phosphatidic acid through abscisic acid signaling pathway in guard cells. (2009)
  • Author(s): Takemiya, A., Shimazaki, K.
  • Organizer: 15^<th> International Congress on Photobiology
  • Place of Presentation: Dusseldorf, Germany
• [Presentation] Type 1 protein phosphatase is a crosstalk point between phototropin and abscisic acid signaling in guard cells. (2009)
  • Author(s): Shimazaki, K., Takemiya, A.
  • Organizer: 15^<th> International Congress on Photobiology
  • Place of Presentation: Dusseldorf, Germany
• [Presentation] Protein phosphatase 1 is a central regulator of blue light-dependent stomatal opening mediated by phototropins (2009)
  • Author(s): Takemiya, A., Shimazaki, K.
  • Organizer: Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
  • Place of Presentation: 京都大学
• [Remarks] ホームページ等
  • URL: http://cellbio.biology.kyushu-u.ac.jp/shimazaki