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Symbiosis-related genes of the nitrogen-fixing bacterium Frankia

Research Project

Project/Area Number 21770053
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionKagoshima University

Principal Investigator

KUCHO Ken-ichi  鹿児島大学, 大学院・理工学研究科, 准教授 (70404473)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords共生 / 窒素固定 / 形質転換 / マイクロアレイ / フランキア / 部位特異的組換 / バイサルファイト / DNAメチル化 / 制限修飾系 / 次世代シーケンサー / 根粒
Research Abstract

Frankia is a nitrogen-fixing bacterium having an ability to make symbiosis with trees. Symbiosis occurs in nodules formed on roots in which Frankia fixes nitrogen and supplies the products to the host plant to promote its growth. In order to identify Frankia genes involved in the symbiosis, I attempted to establish transformation system of Frankia and globally identified Frankia genes induced in nodule.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (45 results)

All 2012 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (32 results) Book (1 results)

  • [Journal Article] Characteristics of Bacteroids in Indeterminate Nodules of the Leguminous Tree Leucaena glauca2011

    • Author(s)
      Ishihara, H.
    • Journal Title

      Microbes and Environments

      Volume: 26 Issue: 2 Pages: 156-159

    • DOI

      10.1264/jsme2.ME11104

    • NAID

      10029122935

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitric oxides production induced in roots of Lotus japonicas by lipopolys accharide from Mesorhizobium loti2011

    • Author(s)
      Murakami, E.
    • Journal Title

      Plant Cell Physiology

      Volume: 52 Issue: 4 Pages: 610-617

    • DOI

      10.1093/pcp/pcr020

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Determinants of the Actinorhizal Symbiosis2010

    • Author(s)
      Kucho K, Hay A-E and Normand P.
    • Journal Title

      Microbes and Environments

      Volume: 25 Issue: 4 Pages: 241-252

    • DOI

      10.1264/jsme2.ME10143

    • NAID

      10027419561

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Frankia alni symbiotic transcriptome2010

    • Author(s)
      Alloisio N, Queiroux C, Fournier P, Pujic P, Normand P, Vallenet D, Medigue C, Yamaura M, Kakoi K and Kucho K.
    • Journal Title

      Mol Plant Microbe Interact

      Volume: 23 Issue: 5 Pages: 593-607

    • DOI

      10.1094/mpmi-23-5-0593

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions2010

    • Author(s)
      Yamaura M, Uchiumi T, Higashi S, Abe M, Kucho K.
    • Journal Title

      Appl Environ Microbiol

      Volume: 76 Issue: 5 Pages: 1962-1964

    • DOI

      10.1128/aem.01813-09

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of abscisic acid on symbiotic nitrogen fixation activity in the root nodules of Lotus japonicus2010

    • Author(s)
      Tominaga A, Nagata M, Futsuki K, Uchiumi T, Abe M, Kucho K, Hashiguchi M, Akashi R, Hirsch AM, Arima S, Suzuki A
    • Journal Title

      Plant Signaling Behavior

      Volume: 5 Pages: 440-443

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Frankia alni Symbiotic Transcriptome2010

    • Author(s)
      Alloisio N, Queiroux C, Fournier P, Pujie P, Normand P, Vallenet D, Medigue C, Yamaura M, Kakoi K, Kucho K
    • Journal Title

      Mol Plant Microbe Interact (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plant peptides govern terminal differentiation of bacteria in symbiosis2010

    • Author(s)
      Van de Velde W, Zehirov G, Szatmari A, Debreczeny M, Ishihare H, Kevei Z, Farkas H, Satiat-Jeunemaitre B, Alunni B, Bourge M, Kucho K, Abe M, Kereszt A, ,Maroti G, Uchiumi T, Kondorosi E, Mergaert P
    • Journal Title

      Science 327

      Pages: 1122-1126

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transient transformation of Frankia by fusion marker genes in liquid culture2009

    • Author(s)
      Kucho K, Kakoi K, Yamaura M, Higashi S, Uchiumi T and Abe M.
    • Journal Title

      Microbes Environ

      Volume: 24 Pages: 231-240

    • NAID

      10025920760

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions2009

    • Author(s)
      Yamaura M, Uchiumi T, Higashi S, Abe M, Kucho K
    • Journal Title

      Appl Environ Microbiol 76

      Pages: 1962-1964

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced nodulation and nitrogen fixation in the ABC low-sensitive mutantenf1 (enhanced nitrogen fixation 1) of Lotus japonicus2009

    • Author(s)
      Tominaga A, Nagata M, Futsuki K, Abe H, Uchiumi T, Abe M, Kucho KI, Hashiguchi M, Akashi R, Hirsch AM, Arima S, Suzuki A
    • Journal Title

      Plant Physiol 151

      Pages: 1965-1976

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transient transformation of Frankia by fusion marker genes in liquid culture2009

    • Author(s)
      Kucho K, Kakoi K, Yamaura M, Higashi S, Uchiumi T Abe M
    • Journal Title

      Microbes Environ 24

      Pages: 231-240

    • NAID

      10025920760

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 共生窒素固定放線菌フランキアのゲノム構造の多様性2012

    • Author(s)
      九町健一、笹川英夫、山中高史、阿部美紀子、内海俊樹
    • Organizer
      第6回日本ゲノム微生物学会年会
    • Place of Presentation
      立教大学
    • Year and Date
      2012-03-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] 樹木と共生する窒素固定放線菌フランキアのゲノム解析2011

    • Author(s)
      九町健一
    • Organizer
      科学技術交流財団研究会「ハイスループットスクーニングと次世代DNAシーケンサーの相乗的応用」
    • Place of Presentation
      愛知
    • Year and Date
      2011-11-11
    • Related Report
      2011 Final Research Report
  • [Presentation] 樹木と共生する窒素固定放線菌フランキアのゲノム解析2011

    • Author(s)
      九町健一
    • Organizer
      科学技術交流財団研究会
    • Place of Presentation
      名古屋大学(招待講演)
    • Year and Date
      2011-11-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] プラスミドを用いたフランキアの形質転換2011

    • Author(s)
      松井勇磨, 栫健太郎, Peter Pujic, 東四郎, 阿部美紀子, Philippe Normand, 内海俊樹, 九町健一
    • Organizer
      植物微生物研究会第21回研究交流会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 次世代シークエンサーを用いた共生窒素固定放線菌フランキアのゲノム解析2011

    • Author(s)
      九町健一, 笹川英夫, 東四郎, 阿部美紀子, 内海俊樹
    • Organizer
      植物微生物研究会第21回研究交流会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] プラスミドを用いたフランキアの形質転換2011

    • Author(s)
      松井勇磨, 栫健太郎, Peter Pujic, 東四郎, 阿部美紀子, Philippe Normand, 内海俊樹, 九町健一
    • Organizer
      植物微生物研究会第21回研究交流会
    • Place of Presentation
      岡山
    • Year and Date
      2011-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 次世代シークエンサーを用いた共生窒素固定放線菌フランキアのゲノム解析2011

    • Author(s)
      九町健一、笹川英夫、阿部美紀子、内海俊樹
    • Organizer
      第5回日本ゲノム微生物学会年会
    • Place of Presentation
      (震災により要旨集発行で発表とみなされた)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Isolation of uracil auxotrophic mutants in Frankia2010

    • Author(s)
      Yamaura M, Uchiumi T, Higashi S, Abe M and Kucho K.
    • Organizer
      1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Miyazaki
    • Year and Date
      2010-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Symbiotic transcriptome of the nitrogen-fixing actinobacterium Frankia2010

    • Author(s)
      Kucho K, Alloisio N, Queiroux C, Fournier P, Pujic P, Yamaura M, Kakoi, K, Uchiumi T, Abe M and Normand P.
    • Organizer
      1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Miyazaki
    • Year and Date
      2010-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Isolation of uracil auxotrophic mutants in Frankia2010

    • Author(s)
      Yamaura M, Uchiumi T, Higashi S, Abe M and Kucho K.
    • Organizer
      16th International Meeting on Frankia and Actinorhizal Plants
    • Place of Presentation
      ポルト
    • Year and Date
      2010-09-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Use of high-activity promoters and a codon-optimized marker gene for Frankia transformation2010

    • Author(s)
      Yamaura M, Higashi S, Uchiumi T, Abe M and Kucho K.
    • Organizer
      16th International Meeting on Frankia and Actinorhizal Plants
    • Place of Presentation
      ポルト
    • Year and Date
      2010-09-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Recent trials for Frankia transformation2010

    • Author(s)
      Yamaura M, Kakoi K, Iwashita M, Abe M, Uchiumi T and Kucho K.
    • Organizer
      16th International Meeting on Frankia and Actinorhizal Plants
    • Place of Presentation
      ポルト
    • Year and Date
      2010-09-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Genome analysis of Frankia by next generation sequencer2010

    • Author(s)
      Kucho K, Abe M, Uchiumi T
    • Organizer
      16th International Meeting on Frankia and Actinorhizal Plants
    • Place of Presentation
      ポルトガル、ポルト
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Recent trials for Frankia transformation2010

    • Author(s)
      Yamaura M, Kakoi K, Iwashita M, Abe M, Uchiumi T, Kucho K
    • Organizer
      16th International Meeting on Frankia and Actinorhizal Plants
    • Place of Presentation
      ポルトガル、ポルト
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Use of high-activity promoters and a codon-optimized marker gene for Frankia transformation2010

    • Author(s)
      Kakoi K, Yamaura M, Higashi S, Uchiumi T, Abe M, Kucho K
    • Organizer
      16th International Meeting on Frankia and Actinorhizal Plants
    • Place of Presentation
      ポルトガル、ポルト
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Isolation of uracil auxotrophic mutants in Frankia2010

    • Author(s)
      Yamaura M, Uchiumi T, Higashi S, Abe M, Kucho K
    • Organizer
      1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      宮崎県宮崎市
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Symbiotic transcriptome of the nitrogen fixing actinobacterium Frankia2010

    • Author(s)
      Kucho K, Alloisio N, Queiroux C, Fournier P, Pujic P, Yamaura M, Kakoi, K, Uchiumi T, Abe M, Normand P
    • Organizer
      1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      宮崎県宮崎市
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Genomic analysis of the actinorhizal symbiosis partners : Frankia alni and Alnus glutinosa2010

    • Author(s)
      Aloisio N, Pujic P, Fournier P, Kucho K, Hocher V, Bogusz D, Normand P
    • Organizer
      XIII National Meeting of the Spanisha Society of Nitrogen Fixation and II Portuguese-Spanish Congress on Nitrogen Fixatio
    • Place of Presentation
      スペイン、ザラゴザ
    • Year and Date
      2010-06-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 樹木と共生窒素固定を行う放線菌フランキアのトランスクリプトーム解析2010

    • Author(s)
      九町健一、Alloisio N、Queiroux C、Fournier P、Pujic P、Vallenet D、Medigue C、山浦真稔、栫健太郎、内海俊樹、阿部美紀子、Normand P
    • Organizer
      日本植物生理学会第51回大会
    • Place of Presentation
      熊本大学(熊本県)
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] 樹木と共生窒素固定を行う放線菌フランキアのトランスクリプトーム解析2010

    • Author(s)
      九町健一、Alloisio N、Queiroux C、Fournier P、Pujic P、Vallenet D、Medigue C、山浦真稔、栫健太郎、内海俊樹、阿部美紀子、Normand P.
    • Organizer
      日本植物生理学会第51回大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Nitrogen-fixing Symbiosis between the Actinobacterium Frankia and Trees2010

    • Author(s)
      Kucho K.
    • Organizer
      7th Okazaki Biology Conferences
    • Place of Presentation
      静岡
    • Year and Date
      2010-01-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Nitrogen-fixing Symbiosis between the Actinobacterium Frankia and Trees2010

    • Author(s)
      Kucho K
    • Organizer
      7th Okazaki Biology Conferences
    • Place of Presentation
      ヤマハリゾートつま恋(静岡)
    • Year and Date
      2010-01-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用2009

    • Author(s)
      栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      植物微生物研究会第19回研究交流会
    • Place of Presentation
      長野
    • Year and Date
      2009-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用2009

    • Author(s)
      栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      植物微生物研究会第19回研究交流会
    • Place of Presentation
      あがたの森文化会館(長野県)
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 単生で窒素固定するFrankiaで誘導される遺伝子の同定2009

    • Author(s)
      山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      植物微生物研究会第19回研究交流会
    • Place of Presentation
      あがたの森文化会館(長野県)
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Transformation of Frankia sp. strain CcI3 by fusion marker genes using liquid culture selection2009

    • Author(s)
      Kucho K, Kakoi K, Yamaura M, Higashi S, Uchiumi T, Abe M.
    • Organizer
      XIV International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      ケベック
    • Year and Date
      2009-07-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 共生窒素固定細菌フランキアの形質転換法の確立2009

    • Author(s)
      九町健一、栫健太郎、山浦真稔、内海俊樹、阿部美紀子
    • Organizer
      2009年度日本放線菌学会大会
    • Place of Presentation
      秋田
    • Year and Date
      2009-07-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用2009

    • Author(s)
      栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      第24回日本放線菌学会
    • Place of Presentation
      秋田
    • Year and Date
      2009-07-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Suppression subtractive hybtidization法の細菌への応用単生で窒素固定を行うFrankiaで特異的に誘導される遺伝子の探索2009年度2009

    • Author(s)
      山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      日本放線菌学会大会
    • Place of Presentation
      秋田
    • Year and Date
      2009-07-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 共生窒素固定細菌フランキアの形質転換法の確立2009

    • Author(s)
      九町健一、栫健太郎、山浦真稔、内海俊樹、阿部美紀子
    • Organizer
      2009年度 日本放線菌学会大会
    • Place of Presentation
      秋田ビューホテル(秋田県)
    • Year and Date
      2009-07-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Suppression subtractive hybtidization法の細菌への応用-単生で窒素固定を行うFrankiaで特異的に誘導される遺伝子の探索2009

    • Author(s)
      栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      2009年度 日本放線菌学会大会
    • Place of Presentation
      秋田ビューホテル(秋田県)
    • Year and Date
      2009-07-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用2009

    • Author(s)
      山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
    • Organizer
      2009年度 日本放線菌学会大会
    • Place of Presentation
      秋田ビューホテル(秋田県)
    • Year and Date
      2009-07-17
    • Related Report
      2009 Annual Research Report
  • [Book] 土壌微生物実験法2012

    • Author(s)
      九町健一、笹川英夫
    • Publisher
      養賢堂(印刷中)
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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