Functional analysis of NADPH oxidase, Rboh protein on pollen tube tip growth
| Project/Area Number |
21770054
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
KAYA Hidetaka Tokyo University of Science, 理工学部, 助教 (80398825)
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| Research Collaborator |
KIMURA Sachie 東京理科大学, 大学院・理工学研究科, 博士課程
KAWARAZAKI Tomoko 東京理科大学, 大学院・理工学研究科, 博士課程
SENZAKI Eriko 東京理科大学, 大学院・理工学研究科, 修士課程
MICHIKAWA Masataka 東京理科大学, 大学院・理工学研究科, 修士課程
IMAI Aya 東京理科大学, 大学院・理工学研究科, 修士課程
NIBORI Hitomi 東京理科大学, 大学院・理工学研究科, 修士課程
NAKAJIMA Ryo 東京理科大学, 大学院・理工学研究科, 修士課程
IIZUKA Ayako 東京理科大学, 理工学部, 学士課程
MORI Kyo-Ichiro 東京理科大学, 理工学部, 学士課程
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 植物分子 / 形態形成 / シロイヌナズナ / 活性酸素種 / シグナル伝達 / 酵素 / 花粉管 / 植物 |
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| Research Abstract |
This study has been focused on the functions of NADPH oxidase on pollen tube growth. I analyzed the functions of AtRbohH and AtRbohJ genes that encode NADPH oxidase in Arabidopsis. I showed that both proteins have ROS (reactive oxygen species)-producing activities as NADPH oxidase using a heterologous expression system. Also I showed that both genes specifically expressed in pollen. atrbohH and atrbohJ double mutant was defective in pollen tube tip growth. These results suggest that ROS produced by AtRbohH and AtRbohJ play a critical role in the pollen tube tip growth.
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Report
(3 results)
Research Products
(6 results)
