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Analysis of alternative splicing mechanisms coordinately regulated by high-light responsive SR proteins

Research Project

Project/Area Number 21770055
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionChubu University

Principal Investigator

YOSHIMURA Kazuya  Chubu University, 応用生物学部, 講師 (90379561)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords環境応答 / 選択的スプライシング / 植物 / SRタンパク質 / 環境ストレス
Research Abstract

We have demonstrated that Arabidopsis SR proteins, atSR45a and atSR30 participates in a spliceosomal assembly through other splicing factors and its expression is markedly induced by high-light stress. These facts suggest that atSR45a and atSR30 are involved in the regulation of high-light stress responsive-alternative splicing events. Here, we identified genes of which alternative splicing efficiencies are regulated by atSR45a and atSR30, by a whole genome tiling array technology, and demonstrated the involvement of the domains in atSR30 and atSR45a in subcellular and subnuclear distribution using a series of structural domain-deleted mutants.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (39 results)

All 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 15 results) Presentation (20 results) Book (2 results)

  • [Journal Article] Dynamic changes in the leaf proteome of a C_3 xerophyte, Citrullus lanatus (wild watermelon), in response to water deficit.2011

    • Author(s)
      Akashi, K.
    • Journal Title

      Planta

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis NADPH oxidases, AtrbohD and AtrbohF, are essential for jasmonic acid-induced expression of genes regulated by MYC2 transcription factor.2011

    • Author(s)
      Maruta, T.
    • Journal Title

      Plant Science

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct regulation of Arabidopsis ADP-ribose/NADH pyrophosphohydrolases, AtNUDX6 and 7, in biotic and abiotic stress responses.2010

    • Author(s)
      Ishikawa, K., Yoshimura, K., Ogawa, T., Shigeoka, S.
    • Journal Title

      Plant Signaling & Behavior(These authors contributed equally to this work.) 5

      Pages: 695-697

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] AtNUDX6, an ADP-ribose/NADH pyrophosphohydrolase in Arabidopsis, positively regulates NPR1-dependent salicylic acid signaling.2010

    • Author(s)
      Ishikawa, K., Yoshimura, K., Harada, K., Fukusaki, E., Ogawa, O., Tamoi, M., Shigeoka, S.
    • Journal Title

      Plant Physiology(These authors contributed equally to this work.) 152

      Pages: 2000-2012

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis chloroplastic ascorbate peroxidase isoenzymes play a dual Role in photoprotection and gene regulation under photooxidative stress.2010

    • Author(s)
      Maruta, T., Tanouchi, A., Tamoi, M., Yabuta, Y., Yoshimura, K., Ishikawa, T., Shigeoka, S.
    • Journal Title

      Plant Cell Physiology 51

      Pages: 190-200

    • NAID

      10027346561

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 高等植物におけるNudix hydrolaseファミリーの多様な生理機能-ヌクレオシド2-リン酸類縁体の代謝を介した細胞応答制御-2010

    • Author(s)
      吉村和也、石川和也、重岡成
    • Journal Title

      化学と生物 48

      Pages: 305-308

    • NAID

      10026438283

    • Related Report
      2010 Final Research Report
  • [Journal Article] AtNUDX6, an ADP-ribose/NADH pyrophosphohydrolase in Arabidopsis, positively regulates NPR1-dependent salicylic acid signaling.2010

    • Author(s)
      Ishikawa, K.
    • Journal Title

      Plant Physiology

      Volume: 152 Pages: 2000-2012

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct regulation of Arabidopsis ADP-ribose/NADH pyrophosphohydrolases, AtNUDX6 and 7, in biotic and abiotic stress responses.2010

    • Author(s)
      Ishikawa, K.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 5 Pages: 695-697

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高等植物におけるNudix hydrolaseファミリーの多様な生理機能-ヌクレオシド2-リン酸類縁体の代謝を介した細胞応答制御-2010

    • Author(s)
      吉村和也
    • Journal Title

      Planta

      Volume: 48 Pages: 305-308

    • NAID

      10026438283

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Modulation of the poly(ADP-ribosyl)ation reaction via the Arabidopsis ADP-ribose/NADH pyrophosphohydrolase, AtNUDX7, is involved in the response to oxidative stress.2009

    • Author(s)
      Ishikawa, K., Ogawa, T., Hirosue, E., Nakayama, Y., Harada, K., Fukusaki, E., Yoshimura, K., Shigeoka, S.
    • Journal Title

      Plant Physiology 151

      Pages: 741-754

    • NAID

      130006991170

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plant-specific SR-related protein atSR45a interacts with spliceosomal proteins in plant nucleus.2009

    • Author(s)
      Tanabe, N., Kimura, A., Yoshimura, K., Shigeoka, S.
    • Journal Title

      Plant Molecular Biology 70

      Pages: 241-252

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of an ADP-ribose pyrophosphatase, AtNUDX2, confers enhanced tolerance to oxidative stress on Arabidopsis plants.2009

    • Author(s)
      Ogawa, T., Ishikawa, K., Harada, K., Fukusaki, E., Yoshimura, K., Shigeoka, S.
    • Journal Title

      Plant Journal 57

      Pages: 289-301

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plant-specific SR-related protein atSR45a interacts with spliceosomal proteins in plant nucleus2009

    • Author(s)
      Tanabe, N.
    • Journal Title

      Plant Molecular Biology 70

      Pages: 241-252

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of the poly(ADP-ribosyl)ation reaction via the Arabidopsis ADP-ribose/NADH pyrophosphohydrolase, AtNUDX7, is involved in the response to oxidative stress2009

    • Author(s)
      Ishikawa, K.
    • Journal Title

      Plant Physiology 151

      Pages: 741-754

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis chloroplastic ascorbate peroxidase isoenzymes playa dual Role in photoprotection and gene regulation under photoo xidative stress2009

    • Author(s)
      Maruta, T.
    • Journal Title

      Plant Cell Physiology 51

      Pages: 190-200

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis NADPH oxidases, AtrbohD and AtrbohF, are essential for jasmonic acid-induced expression of genes regulated by MYC2 transcription factor.

    • Author(s)
      Maruta, T., Inoue, T., Tamoi, M., Yabuta, Y., Yoshimura, K., Ishikawa, T., Shigeoka, S.
    • Journal Title

      Plant Sci. in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic changes in the leaf proteome of a C3 xerophyte, Citrullus lanatus (wild watermelon), in response to water deficit.

    • Author(s)
      Akashi, K., Yoshida, K., Kuwano, M., Kajikawa, M., Yoshimura, K., Hoshiyasu, S., Inagaki, N., Yokota, A.
    • Journal Title

      Planta in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] 強光応答性選択的スプライシング制御因子atSR30およびatSR45aの核局在化の解析2011

    • Author(s)
      森達也、吉村和也、小池佳之、田部記章、丸田隆典、重岡成
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 強光応答性選択的スプライシング制御因子atSR30およびatSR45aの核局在化の解析2011

    • Author(s)
      吉村和也
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学(震災により開催中止、大会は成立)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナASF/SF2様タンパク質、atSR30による強光ストレスに応答した選択的スプライシングの制御2011

    • Author(s)
      横山国大、森達也、田部記章、丸田隆典、佐藤信雄、高橋広夫、重岡成、吉村和也
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 強光応答性SRタンパク質atSR45aの選択的スプライシング制御に果たす役割2011

    • Author(s)
      森達也、田部記章、丸田隆典、横山国大、佐藤信雄、高橋広夫、吉村和也、重岡成
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナASF/SF2様タンパク質、atSR30による強光ストレスに応答した選択的スプライシングの制御2011

    • Author(s)
      横山国大
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(震災により開催中止、年会は成立)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 強光応答性SRタンパク質atSR45aの選択的スプライシング制御に果たす役割2011

    • Author(s)
      森達也
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(震災により開催中止、年会は成立)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナASF/SF2様SRタンパク質、atSR30による強光ストレスに応答した選択的スプライシング制御機構の解析2010

    • Author(s)
      横山国大、石川裕基、森達也、田部記章、丸田隆典、佐藤信雄、高橋広夫、吉村和也、重岡成
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] タイリングアレイを用いた強光応答性SRタンパク質、atSR45aにより選択的スプライシング効率が制御される遺伝子の同定2010

    • Author(s)
      森達也、田部記章、丸田隆典、横山国大、佐藤信雄、高橋広夫、吉村和也、重岡成
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 強光応答性SRタンパク質、atSR30およびatSR45aの核局在化に関与する機能ドメインの解析2010

    • Author(s)
      森達也、田部記章、丸田隆典、吉村和也、重岡成
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナASF/SF2様SRタンパク質、atSR30による強光ストレスに応答した選択的スプライシング制御機構の解析2010

    • Author(s)
      横山国大
    • Organizer
      第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] タイリングアレイを用いた強光応答性SRタンパク質、atSR45aにより選択的スプライシング効率が制御される遺伝子の同定2010

    • Author(s)
      森達也
    • Organizer
      第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 強光応答性SRタンパク質、atSR30およびatSR45aの核局在化に関与する機能ドメインの解析2010

    • Author(s)
      吉村和也
    • Organizer
      第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of alternative splicing events of genes regulated by Arabidopsis serine/arginine proteins, atSR45a and atSR30, in response to high-light stress using a tiling microarray.2010

    • Author(s)
      Yoshimura, K., Mori, T., Yokoyama, K., Maruta, T., Tanabe, N., Shigeoka, S.
    • Organizer
      21st International Cnference on Arabidopsis Research, 2010, at Yokohama
    • Place of Presentation
      Japan
    • Year and Date
      2010-06-07
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of alternative splicing events of genes regulated by Arabidopsis serine/arginine proteins, atSR45a and atSR30, in response to high-light stress using a tiling microarray.2010

    • Author(s)
      Yoshimura, K.
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-06-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] タイリングアレイを用いた強光応答性シロイヌナズナSRタンパク質、atSR45aの選択的スプライシング制御機構の解析2010

    • Author(s)
      森達也、森雅揮、横山国大、田部記章、丸田隆典、佐藤信雄、高橋広夫、吉村和也、重岡成
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] タイリングアレイを用いた強光応答性シロイヌナズナSRタンパク質、atSR45aの選択的スプライシング制御機構の解析2010

    • Author(s)
      森達也
    • Organizer
      日本農芸化学会 2010年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 強光ストレス応答性SRタンパク質、atSR30による選択的スプライシング制御機構の解析2010

    • Author(s)
      横山国大、石川裕基、吉村和也、森達也、田部記章、丸田隆典、佐藤信雄、高橋広夫、重岡成
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-19
    • Related Report
      2010 Final Research Report
  • [Presentation] 強光ストレス応答性SRタンパク質、atSR30による選択的スプライシング制御機構の解析2010

    • Author(s)
      吉村和也
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学(熊本市)
    • Year and Date
      2010-03-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物の選択的スプライシング制御因子atSR30およびatSR45aによる強光ストレス応答2009

    • Author(s)
      森達也、田部記章、丸田隆典、吉村和也、重岡成
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物の選択的スプライシング制御因子atSR30およびatSR45aによる強光ストレス応答2009

    • Author(s)
      森達也
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Book] ビタミン総合事典5-2-3植物におけるパントテン酸の生合成(日本ビタミン学会 編集)2010

    • Author(s)
      吉村和也
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] ビタミン総合事典5-2-3植物におけるパントテン酸の生合成日本ビタミン学会編集2010

    • Author(s)
      吉村和也
    • Publisher
      朝倉書店
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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