Expression profiling of seed-specific pre-miRNA binding to Arabidopsis DCL1 protein

• Project/Area Number: 21770056
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: TATEMATSU Kiyoshi 基礎生物学研究所, 植物器官形成学研究室, 助教 (00373324)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: シロイヌナズナ / 機能性小分子RNA / pre-miRNA / AtDCL1タンパク質 / 種子休眠・発芽 / DCL1遺伝子 / HYL1遺伝子 / 過剰発現株

Research Abstract

To isolate pre-miRNAs binding to DCL1 from Arabidopsis, we made transgenic Arabidopsis plants, which express the DCL1 fused with a tag sequence driven by the inducible promoter. We confirmed the expression level of the fusion DCL1 gene using RT-PCR and western bolt analyses. Finally, we are preparing the pre-miRNAs binding to the fused DCL1 protein from the transgenic plants by immunoprecipitation methods

Research Products

• [Journal Article] Tissue-specific transcriptome analysis reveals cell wall metabolism, flavonol biosynthesis, and defense responses are activated in the endosperm of germinating Arabidopsis thaliana seeds. (2012)
  • Author(s): Endo, A.*, Tatematsu, K.*, Hanada, K.*, Duermeyer, L., Okamoto, M., Yonekura-Sakakibara, K., Saito, K., Toyoda, T., Kawakami, N., Kamiya, Y., Seki, M., and Nambara, E.
  • Journal Title: Plant Cell Physiol. Volume: 53, (*: Equally contributed) Pages: 16-27
  • NAID: 10030306944
  • Peer Reviewed
• [Journal Article] Abscisic acid and the control of seed dormancy and germination. (2010)
  • Author(s): Nambara, E., Okamoto, M., Tatematsu, K., Yano, R., Seo, M., Kamiya, Y.
  • Journal Title: Seed Sci. Res. Volume: 20 Pages: 55-67
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of endogenous abscisic acid-mediated transcription in Arabidopsis seeds using tiling arrays. (2010)
  • Author(s): Okamoto, M., Tatematsu, K., Matsui, A., Morosawa, T., Ishida, J., Tanaka, M., Endo, T., Mochizuki, Y., Toyoda, T., Kamiya, Y., Shinozaki, K., Nambara E., and Seki, M.
  • Journal Title: Plant J. Volume: 62 Pages: 39-51
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of endogenous abscisic acid-mediated transcription in Arabidopsis seeds using tiling arrays (2010)
  • Author(s): Okamoto, et al
  • Journal Title: Plant J. Volume: 62 Pages: 39-51
  • Peer Reviewed
• [Journal Article] Abscisic acid and the control of seed dormancy and germination (2010)
  • Author(s): Nambara, et al
  • Journal Title: Seed Science Research Volume: 20 Pages: 55-67
  • Peer Reviewed
• [Journal Article] Temporal expression patterns of hormone metabolism genes during imbibition of Arabidopsis thaliana Seeds: a comparative study on dormant and non-dormant accessions. (2009)
  • Author(s): Preston, J.*, Tatematsu, K.*, Kanno, Y., Hobo, T., Kimura, M., Jikumaru, Y., Yano, R., Kamiya, Y., and Nambara, E.
  • Journal Title: Plant Cell Physiol. Volume: 50, (*: Equally contributed) Pages: 1786-1800
  • NAID: 80020607108
  • Peer Reviewed
• [Journal Article] Nitrate responses of Arabidopsis cho1 mutants: obvious only when excess nitrate is supplied. (2009)
  • Author(s): Nambara, E., Yamagishi, K., Tatematsu, K., Nakabayashi, K., and Kamiya, Y.
  • Journal Title: Plant Signal. Behav. Volume: 4 Pages: 1164-1166
• [Journal Article] Temporal expression patterns of hormone metabolism genes during imbibition of Arabidopsis thaliana Seeds : a comparative study on dormant and non-dormant accessions (2009)
  • Author(s): Preston, Tatematsu et al.
  • Journal Title: Plant and Cell Physiology 50 Pages: 1786-1800
  • NAID: 80020607108
  • Peer Reviewed
• [Journal Article] Nitrate responses of Arabidopsis cho1 mutants : obvious only when excess nitrate is supplied (2009)
  • Author(s): Nambara et al.
  • Journal Title: Plant Singal.Behav. 4 Pages: 1164-1166
• [Presentation] Analysis of the genes that expressed in endosperms after imbibition of Arabidopsis seeds. (2011)
  • Author(s): Nambara, E., Tatematsu K., Hanada, K., Okamoto, M., Endo, A., Seki, M., and Kamiya, Y.
  • Organizer: Canadian Plant Genomic Workshop 2011
  • Place of Presentation: Niagara Falls, Canada
• [Presentation] miR165/166 might regulate non-cell-autonomously the expression domains of HD-Zip III in Arabidopsis leaf primordia. (2010)
  • Author(s): Tatematsu, K.
  • Organizer: The 2nd NIBB-MPIPZ Joint Symposium Plant Science Communications 2010
  • Place of Presentation: NIBB, Okazaki, Japan
• [Presentation] Studies of ABA action on Arabidopsis seed germination (2010)
  • Author(s): Tatematsu, K., Nakabayashi, K., Osuna, D., Fernandez-Arbaizar, A., Albertos-Arranz, P., Kamiya, Y., Lorenzo, O., and Nambara, E.
  • Organizer: 3rd ISSS Workshop on Molecular Aspects of Seed Dormancy and Germination
  • Place of Presentation: York, UK
• [Presentation] Studies of ABA action on Arabidopsis seed germination (2010)
  • Author(s): Tatematsu K., et al
  • Organizer: 3rd ISSS Workshop on Molecular Aspects of Seed Dormancy and Germination
  • Place of Presentation: イギリス ヨーク
• [Presentation] miR165/166 might regulate non-cell-autonomously the expression domains of HD-Zip III in Arabidopsis leaf primordia (2010)
  • Author(s): Tatematsu K.
  • Organizer: The 2nd NIBB-MPIPZ Joint Symposium Plant Science Communications 2010
  • Place of Presentation: 岡崎基礎生物学研究所
• [Presentation] シロイヌナズナ種子における吸水極初期の植物ホルモン代謝 (2009)
  • Author(s): 南原英司, 立松圭, 菅野裕理, 保浦徳昇, 軸丸裕介, 神谷勇治
  • Organizer: 植物化学調節学会第４４回大会
  • Place of Presentation: 東北大学, 仙台
• [Presentation] The role of miRNA in leaf development along the adaxial-abaxial axis in Arabidopsis thaliana. (2009)
  • Author(s): Tatematsu, K.
  • Organizer: Japanese-German Symposium on Evolution and Development
  • Place of Presentation: Cologne, Germany
• [Presentation] シロイヌナズナ種子における吸水極初期の植物ホルモン代謝 (2009)
  • Author(s): 南原, ら
  • Organizer: 植物化学調節学会第44回大会
  • Place of Presentation: 仙台東北大学
• [Remarks] 自然科学研究機構基礎生物学研究所 一般公開「体験！生き物研究空間」 体験実験「生き物をDNA鑑定してみよう-PCR法による遺伝子解析-」, 20101002
• [Remarks] 自然科学研究機構基礎生物学研究所 高校生実験講座2010 「葉と花の生まれるところを見てみよう」 （共催：日本学術振興会 H22年度 ひらめき・ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI） 20100821