Analysis of glutathione metabolic pathway and glutathione-conjugates in plants

• Project/Area Number: 21770058
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: OHTSU Naoko Tokyo University of Agriculture and Technology, 大学院・農学研究院, 助教 (40513437)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 植物 / 硫黄栄養 / グルタチオン / 代謝 / ジャスモン酸 / タンパク精製 / メタボロミクス

Research Abstract

To identify the enzyme degrading glutathione which is a storage form of organic sulfur, the enzyme was purified 500-fold based on its activity. To identify glutathione-conjugates in plants, metabolites from Arabidopsis mutants defective in degrading glutathione-conjugates in the vacuole was analyzed. It was found that 12-Oxo-phytodienoic acid, which is a precursor of plant hormone jasmonic acid, was transported into the vacuole in the form of glutathione-conjugate.

Research Products

• [Journal Article] 12-Oxo-phytodienoic acid-glutathione conjugate is transported into the vacuole in Arabidopsis. (2011)
  • Author(s): Onkama-Ohtsu, N., Sasaki-Sekimoto, Y., Oikawa, A., Jikumaru, Y.Shoko, S., Inoue, E., Kamide, Y., Yokoyama, T., Hirai, M.Y., Shirasu, K., Kamiya, Y., Oliver, D.J., Kazuki Saito
  • Journal Title: Plant and Cell Physiology 52 Pages: 205-209
  • NAID: 130006994257
  • Peer Reviewed
• [Journal Article] 12-Oxo-phytodienoic acid-glutathione conjugate is transported into the vacuole in Arabidopsis. (2011)
  • Author(s): Ohkama-Ohtsu, N., Sasaki-Sekimoto, Y., Oikawa, A., et al.
  • Journal Title: Plant and Cell Physiology Volume: 52 Pages: 205-209
  • NAID: 130006994257
  • Peer Reviewed
• [Journal Article] Recent progress in plant nutrition research : cross-talk between nutrients, plant physiology and soil microorganisms. (2010)
  • Author(s): Onkama-Ohtsu, N., Wasaki, J.
  • Journal Title: Plant and Cell Physiology 51 Pages: 1255-1264
  • Peer Reviewed
• [Journal Article] 硫黄栄養応答機構とグルタチオン代謝の分子生理学的解明 (2010)
  • Author(s): 大鎌直子
  • Journal Title: 日本土壌肥料学雑誌 81 Pages: 461-462
• [Journal Article] Recent progress in plant nutrition research : cross-talk between nutrients, plant physiology and soil microorganisms. (2010)
  • Author(s): Ohkama-ohtsu, N., Wasaki, J.
  • Journal Title: Plant and Cell Physiology Volume: 51 Pages: 1255-1264
  • Peer Reviewed
• [Journal Article] 硫黄栄養応答機構とグルタチオン代謝の分子生理学的解明 (2010)
  • Author(s): 大鎌直子
  • Journal Title: 日本土壌肥料学雑誌 Volume: 81 Pages: 461-462
• [Journal Article] Roles of γ-glutamyl transpeptidase and γ-glutamyl cyclotransferase in glutathione and glutathione-conjugate metabolism in plants. (2009)
  • Author(s): Ohkama-Ohtsu, N., Fukuyama, K., Oliver, D.J.
  • Journal Title: Advances in Botanical Research Volume: 52 Pages: 87-113
  • Peer Reviewed
• [Presentation] 硫黄代謝制御機構と硫黄化合物の生理作用 (2010)
  • Author(s): 大津直子
  • Place of Presentation: 北海道大学農学部
  • Year and Date: 2010-09-09
• [Presentation] 硫黄栄養応答機構とグルタチオン代謝の分子生理学的解明 (2010)
  • Author(s): 大津直子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 北海道大学農学部
  • Year and Date: 2010-09-08
• [Presentation] 植物におけるグルタチオン代謝及び内在性グルタチオン抱合体の研究 (2009)
  • Author(s): 大津直子
  • Place of Presentation: 京都大学
  • Year and Date: 2009-09-16
• [Book] Roles of γglutamyl transpeptidase and γglutamyl cyclotransferase in glutathione and glutathione-conjugate metabolism in plants. 52 (2009)
  • Author(s): Ohkama-Ohtsu, N., Fukuyama, K., Oliver, D.J.
  • Publisher: Advances in Botanical Research
• [Remarks] ホームページ等
  • URL: http://kenkyu-web.tuat.ac.jp/Profiles/32/0003141/profile.html
• [Remarks]
  • URL: http://kenkyu-web.tuat.ac.jp/Profiles/32/0003141/profile.html
• [Remarks]
  • URL: http://kenkyu-web.tuat.ac.jp/Profiles/32/0003141/profile.html