Analysis of glutathione metabolic pathway and glutathione-conjugates in plants
| Project/Area Number |
21770058
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
OHTSU Naoko Tokyo University of Agriculture and Technology, 大学院・農学研究院, 助教 (40513437)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 植物 / 硫黄栄養 / グルタチオン / 代謝 / ジャスモン酸 / タンパク精製 / メタボロミクス |
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| Research Abstract |
To identify the enzyme degrading glutathione which is a storage form of organic sulfur, the enzyme was purified 500-fold based on its activity. To identify glutathione-conjugates in plants, metabolites from Arabidopsis mutants defective in degrading glutathione-conjugates in the vacuole was analyzed. It was found that 12-Oxo-phytodienoic acid, which is a precursor of plant hormone jasmonic acid, was transported into the vacuole in the form of glutathione-conjugate.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] 12-Oxo-phytodienoic acid-glutathione conjugate is transported into the vacuole in Arabidopsis.2011
Author(s)
Onkama-Ohtsu, N., Sasaki-Sekimoto, Y., Oikawa, A., Jikumaru, Y.Shoko, S., Inoue, E., Kamide, Y., Yokoyama, T., Hirai, M.Y., Shirasu, K., Kamiya, Y., Oliver, D.J., Kazuki Saito
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Journal Title
Plant and Cell Physiology 52
Pages: 205-209
NAID
Related Report
Peer Reviewed
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