Identification of plant immune signaling factors by differential phosphoproteomics

• Project/Area Number: 21770059
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NAKAGAMI Hirofumi The Institute of Physical and Chemical Research, 植物プロテオミクス研究ユニット, ユニットリーダー (20435663)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 植物免疫 / プロテオミクス / リン酸化 / シグナル伝達 / シロイヌナズナ

Research Abstract

To perform comparative analysis of phosphoproteomes, reciprocal 15N-labeling method has been established. By employing the method, phosphoproteome dynamics upon microbe-associated molecular pattern treatments have been analyzed in Arabidopsis cells. As a result, 682 protein factors, whose phosphorylation status altered upon the stimuli, were identified. Furthermore, novel three plant immune signaling regulators were identified by analyzing knockout mutant plants.

Research Products

• [Journal Article] MASCP Gator : an aggregation portal for the visualization of Arabidopsis proteomics data. (2011)
  • Author(s): Joshi HJ, Hirsch-Hoffmann M, Baerenfaller K, Gruissem W, Baginsky S, Schmidt R, Schulze WX, Sun Q, van Wijk KJ, Egelhofer V, Wienkoop S, Weckwerth W, Bruley C, Rolland N, Toyoda T, Nakagami H, Jones AM, Briggs SP, Castleden I, Tanz SK, Millar AH, Heazlewood JL.
  • Journal Title: Plant Physiol. 155(1) Pages: 259-270
  • Peer Reviewed
• [Journal Article] MASCP Gator : An Aggregation Portal for the Visualization of Arabidopsis Proteomics Data (2011)
  • Author(s): Hiren J.Joshi
  • Journal Title: Plant Physiology Volume: 155(1) Pages: 259-270
  • Peer Reviewed
• [Journal Article] The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana. (2010)
  • Author(s): Kosetsu K, Matsunaga S, Nakagami H, Colcombet J, Sasabe M, Soyano T, Takahashi Y, Hirt H, Machida Y.
  • Journal Title: Plant Cell. 22(11) Pages: 3778-3790
  • Peer Reviewed
• [Journal Article] Large-scale comparative phosphoproteomics identifies conserved phosphorylation sites in plants. (2010)
  • Author(s): Nakagami H, Sugiyama N, Mochida K, Daudi A, Yoshida Y, Toyoda T, Tomita M, Ishihama Y, Shirasu K.
  • Journal Title: Plant Physiol. 153(3) Pages: 1161-1174
  • Peer Reviewed
• [Journal Article] The MAP Kinase MPK4 Is Required for Cytokinesis in Arabidopsis thaliana (2010)
  • Author(s): Ken Kosetsu
  • Journal Title: The Plant Cell Volume: 22(11) Pages: 3778-3790
  • Peer Reviewed
• [Journal Article] Large-scale comparative phosphoproteomics identifies conserved phosphorylation sites in plants (2010)
  • Author(s): Hirofumi Nakagami
  • Journal Title: Plant Physiology Volume: 153(3) Pages: 1161-1174
  • Peer Reviewed
• [Presentation] MAMPsシグナル伝達経路のリン酸化プロテオーム解析 (2011)
  • Author(s): 松井英譲, 野村有子, 加星(岸)光子, 高橋章, 廣近洋彦, 白須賢, 中神弘史
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学府中キャンパス
  • Year and Date: 2011-03-29
• [Presentation] OsPti1aが病害抵抗性抑制因子として機能するためには、細胞膜上で適切な複合体を形成する必要がある (2011)
  • Author(s): 高橋章, 松井英譲, 中神弘史, 野村有子, 廣近洋彦
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学府中キャンパス
  • Year and Date: 2011-03-27
• [Presentation] リン酸化プロテオミクス手法を用いたMAMPシグナル伝達経路の解析 (2011)
  • Author(s): 松井英譲, 野村有子, 加星(岸)光子, 高橋章, 廣近洋彦, 白須賢, 中神弘史
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2011-03-22
• [Presentation] OsPti1aが病害抵抗性抑制因子として機能するためには、細胞膜上で適切な複合体を形成する必要がある (2011)
  • Author(s): 高橋章, 松井英譲, 野村有子, 中神弘史, 廣近洋彦
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2011-03-22
• [Presentation] シロイヌナズナのRNA分解制御を介した低温ストレス応答の研究 (2011)
  • Author(s): 中南健太郎, 千葉由佳子, 松井章浩, 中神弘史, 野村有子, 田中真帆, 諸澤妙子, 石田順子, 関原明
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2011-03-22
• [Presentation] リン酸化プロテオミクス手法を用いたMAMPシグナル伝達経路の解析 (2011)
  • Author(s): 松井英譲
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス、仙台
  • Year and Date: 2011-03-22
• [Presentation] OsPtilaが病害抵抗性抑制因子として機能するためには、細胞膜上で適切な複合体を形成する必要がある (2011)
  • Author(s): 高橋章
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス、仙台
  • Year and Date: 2011-03-22
• [Presentation] シロイヌナズナのRNA分解制御を介した低温ストレス応答の研究 (2011)
  • Author(s): 中南健太郎
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス、仙台
  • Year and Date: 2011-03-22
• [Presentation] MAMPsシグナル伝達経路のリン酸化プロテオーム解析 (2011)
  • Author(s): 中神弘史
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 東京農工大学府中キャンパス、府中
• [Presentation] Phosphoproteomic approach for plant signaling network dissection (2010)
  • Author(s): Hirofumi Nakagami
  • Organizer: Inference and modelling of regulatory networks in multicellular systems, 21st International Conference on Arabidopsis Research Satellite Workshop
  • Place of Presentation: RIKEN Yokohama Institute, Yokohama, Japan
  • Year and Date: 2010-06-11
• [Presentation] Phosphoproteomic in plants (2010)
  • Author(s): Hirofumi Nakagami
  • Organizer: MASC Proteomics Subcommittee Forum at 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama Japan
  • Year and Date: 2010-06-07
• [Presentation] Phosphoproteomics in plants (2010)
  • Author(s): Hirofumi Nakagami
  • Organizer: MASC Proteomics Subcommittee Forum at 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2010-06-07
• [Presentation] リン酸化プロテオーム解析手法を用いた病害抵抗性機構の解析 (2010)
  • Author(s): 中神弘史, 野村有子, 大井信明, 持田恵一, 杉山直幸, 石濱泰, 白須賢
  • Organizer: 平成22年度日本植物病理学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-04-20
• [Presentation] リン酸化プロテオーム解析手法を用いた病害抵抗性機構の解析 (2010)
  • Author(s): 中神弘史
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 国立京都国際会館、京都
  • Year and Date: 2010-04-20
• [Presentation] リン酸化プロテオミクスによる植物免疫シグナリングの解析 (2010)
  • Author(s): 中神弘史, 野村有子, 大井信明, 持田恵一, 杉山直幸, 石濱泰, 白須賢
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪北キャンパス
  • Year and Date: 2010-03-20
• [Presentation] シロイヌナズナの低温脱馴化過程に関与するRNAマスキング制御の研究 (2010)
  • Author(s): 中南健太郎, 南杏鶴, 中神弘史, 田中真帆, 諸澤妙子, 石田順子, 篠崎一雄, 白須賢, 上村松生, 関原明
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪北キャンパス
  • Year and Date: 2010-03-20
• [Presentation] リン酸化プロテオミクスによる植物免疫シグナリングの解析 (2010)
  • Author(s): 中神弘史
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 熊本市
  • Year and Date: 2010-03-20
• [Presentation] Comparative phosphoproteomics in plants for translational research (2010)
  • Author(s): Hirofumi Nakagami, Naoyuki Sugiyama, Yuko Nomura, Keiichi Mochida, Yuko Yoshida, Tetsuro Toyoda, Masaru Tomita, Yasushi Ishihama, Ken Shirasu
  • Organizer: 2^<nd> International Symposium on Frontier in Agriculture Proteome Research
  • Place of Presentation: Tsukuba NORIN Hall
• [Presentation] 植物病害抵抗性のリン酸化プロテオーム解析 (2010)
  • Author(s): 中神弘史, 野村有子, 大井信明, 持田恵一, 杉山直幸, 冨田勝, 石濱泰, 白須賢
  • Organizer: 日本プロテオーム学会2010年会
  • Place of Presentation: 東京ベイホテル東急
• [Presentation] Phosphoproteomic approach for plant signaling network dissection (2010)
  • Author(s): Hirofumi Nakagami
  • Organizer: IMMS Workshop‘Inference and modelling of regulatory networks in multicellular systems', RIKEN Plant Science Center
  • Place of Presentation: Yokohama, Japan
• [Presentation] Comparative phosphoproteomics in plants (2010)
  • Author(s): Hirofumi Nakagami, Naoyuki Sugiyama, Keiichi Mochida, Arsalan Daudi, Yuko Yoshida, Tetsuro Toyoda, Masaru Tomita, Yasushi Ishihama, Ken Shirasu
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
• [Presentation] RNA masking system during cold deacclimation of Arabidopsis plants (2010)
  • Author(s): Kentaro Nakaminami, Anzu Minami, Hirofumi Nakagami, Maho Tanaka, Taeko Morosawa, Junko Ishida, Kazuo Shinozaki, Ken Shirasu, Matsuo Uemura, Motoaki Seki
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
• [Presentation] Comparative phosphoproteomics in plants for translational research (2010)
  • Author(s): Hirofumi Nakagami
  • Organizer: The 2^<nd> International Symposium Frontier in Agriculture Proteome Research
  • Place of Presentation: Tsukuba NORIN Hall, Tsukuba, Japan
• [Presentation] 植物病害抵抗性のリン酸化プロテオーム解析 (2010)
  • Author(s): 中神弘史
  • Organizer: 日本ヒトプロテオーム機構第8回大会
  • Place of Presentation: 東京ベイホテル東急、浦安
• [Presentation] RNA MASHKING SYSTEM DURING COLD DEACCLIMATION OF ArAbidopsis PLANTS (2010)
  • Author(s): Kentaro Nakaminami
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
• [Presentation] COMPARATIVE PHOSPHOPROTEOMICS IN PLANTS (2010)
  • Author(s): Hirofumi Nakagami
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
• [Presentation] Comparative phosphoproteomics of Arabidopsis and rice to understand plant immunity (2009)
  • Author(s): Hirofumi Nakagami
  • Organizer: Tsuruoka Proteomics Meeting 2009
  • Place of Presentation: Tsuruoka Metabolome Campus, Japan
  • Year and Date: 2009-12-07
• [Presentation] Comparative phosphoproteomics of Arabidopsis and rice to understand plant immunity (2009)
  • Author(s): 中神弘史
  • Organizer: Tsuruoka Proteomics Meeting 2009
  • Place of Presentation: 鶴岡市
  • Year and Date: 2009-12-07
• [Presentation] イネとシロイヌナズナにおける比較リン酸化プロテオミクス (2009)
  • Author(s): 中神弘史, 杉山直幸, 持田恵一, Arsalan Daudi, 冨田勝, 石濱泰, 白須賢
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] 植物におけるリン酸化プロテオームの大規模な比較解析 (2009)
  • Author(s): 中神弘史
  • Organizer: 7^<th> JHUPO Conference 2009
  • Place of Presentation: 東京
  • Year and Date: 2009-07-28
• [Presentation] Large-scale comparative phosphoproteomics of Arabidopsis and rice (2009)
  • Author(s): Hirofumi Nakagami, Naoyuki Sugiyama, Keiichi Mochida, Arsalan Daudi, Masaru Tomita, Yasushi Ishihama, Ken Shirasu
  • Organizer: Plant Genomics and Beyond Conference 2009
  • Place of Presentation: URGV, France
  • Year and Date: 2009-07-08
• [Presentation] Large-scale comparative phosphoproteomics of Arabidopsis and rice (2009)
  • Author(s): 中神弘史
  • Organizer: "Plant Genomics and Beyond" Conference 2009
  • Place of Presentation: Evry, フランス
  • Year and Date: 2009-07-08
• [Presentation] 植物におけるリン酸化プロテオームの大規模な比較解析 (2009)
  • Author(s): 中神弘史, 杉山直幸, 持田恵一, Arsalan Daudi, 冨田勝, 石濱泰, 白須賢
  • Organizer: 日本プロテオーム機構第7回大会
  • Place of Presentation: 北里大学
• [Presentation] Identification of novel RNA masking system during deacclimation of Arabidopsis plants (2009)
  • Author(s): Kentaro Nakaminami, Anzu Minami, Hirofumi Nakagami, Nobuaki Ohi, Maho Tanaka, Taeko Morosawa, Junko Ishida, Ken Shirasu, Matsuo Uemura, Motoaki Seki
  • Organizer: Joint Annual Meetings of the American Society of Plant Biologists and the Phycological Society of America
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA
• [Book] 植物リン酸化プロテオーム解析の最前線(植物のシグナル伝達-分子と応答) (2010)
  • Author(s): 中神弘史、杉山直幸、石濱泰
  • Publisher: 共立出版
• [Book] 植物のシグナル伝達-分子と応答- (2010)
  • Author(s): 中神弘史
  • Publisher: 共立出版
• [Remarks] RIKEN Phosphoproteome Database (RIPP-DB)
  • URL: https://database.riken.jp/sw/links/en/ria102i/
• [Remarks] MASCP Gator
  • URL: http://www.masc-proteomics.org/mascp/index.php/Gator
• [Remarks]
  • URL: http://proteomics.psc.riken.jp/