Study of "bulb, subregion-like structure on the vacuolar membrane with a complex configuration

• Project/Area Number: 21770060
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SAITO Chieko The Institute of Physical and Chemical Research, 中野生体膜研究室, 専任研究員 (10321762)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: 液胞 / SNARE / phospholipase / ブラシノステロイド / シロイヌナズナ / 水チャネル / PI3P / オートファジー / ライブイメージング

Research Abstract

I have previously identified and reported a "bulb", a complex and mobile structure on the continuous vacuolar membrane at 2002. To get a clue its biological significance and function, I searched mutants that show abnormalities in bulbs. By a reverse genetical approach, I found that the frequency of the appearance of bulbs were reduced in autophagy mutants. I further tried to isolate mutants that have abnormal bulbs by forward genetical approach.

Research Products

• [Journal Article] The occurrence of bulbs, a complex configuration of the vacuolar membrane, is affected by mutations of vacuolar SNARE and phospholipase in Arabidopsis (2011)
  • Author(s): Saito, C., Uemura, T., Awai, C., Tominaga, M., Ebine, K., Ito, J., Ueda, T., Abe, H., Morita, T.M., Tasaka, M., Nakano, A.
  • Journal Title: Plant J (in press)
  • Peer Reviewed
• [Journal Article] Live-cell imaging reveals the dynamics of two sperm cells during double fertilization in Arabidopsis thaliana. (2011)
  • Author(s): Hamamura, Y., Saito, C., Awai, C., Kurihara, D., Miyawaki, A., Nakagawa, T., Kanaoka, M.M., Sasaki, N., Nakano, A., Berger, F., Higashiyama, T.
  • Journal Title: Curr Biol 21 Pages: 497-502
  • Peer Reviewed
• [Journal Article] Loss-of-function mutations of retromer large subunit genes suppress the phenotype of an Arabidopsis zig mutant that lacks Qb-SNARE VTI11. (2010)
  • Author(s): Hashiguchi, Y., Niihama, M., Takahashi, T., Saito, C., Nakano, A., Tasaka, M., Morita, M.T.
  • Journal Title: Plant Cell 22 Pages: 159-172
  • Peer Reviewed
• [Journal Article] The chloroplast protein BPG2 functions in brassinosteroid-mediated post-transcriptional accumulation of chloroplast rRNA. (2010)
  • Author(s): Komatsu, T., Kawaide, H., Saito, C., Yamagami, A., Shimada, S., Nakazawa, M., Matsui, M., Nakano, A., Tsujimoto, M., Natsume, M., Abe, H., Asami, T., Nakano, T.
  • Journal Title: Plant J 61 Pages: 409-422
  • Peer Reviewed
• [Journal Article] Vacuolar/pre-vacuolar compartment Qa-SNAREs VAM3/SYP22 and PEP12/SYP21 have interchangeable functions in Arabidopsis. (2010)
  • Author(s): Uemura, T., Morita, M.T., Ebine, K., Okatani, Y., Yano, D., Saito, C., Ueda, T., Nakano, A.
  • Journal Title: Plant J 64 Pages: 864-873
  • Peer Reviewed
• [Journal Article] The chloroplast protein BPG2 functions in brassinosteroid-mediated post-transcriptional accumulation of chloroplast rRNA. (2010)
  • Author(s): Komatsu, et al.
  • Journal Title: The Plant J Volume: 61 Pages: 409-422
  • Peer Reviewed
• [Journal Article] Loss-of-Function Mutations of Retromer Large Subunit Genes Suppress the Phenotype of an Arabidopsis zig Mutant That Lacks Qb-SNARE VTI11 (2010)
  • Author(s): Hashiguchi, et al.
  • Journal Title: Plant Cell Volume: 22 Pages: 159-172
  • Peer Reviewed
• [Journal Article] Vacuolar/pre-vacuolar compartment Qa-SNAREs VAM3/SYP22 and PEP12/SYP21 have interchangeable functions in Arabidopsis (2010)
  • Author(s): Uemura, et al.
  • Journal Title: The Plant J Volume: 64 Pages: 864-873
  • Peer Reviewed
• [Journal Article] Application of Lifeact reveals F-actin dynamics in Arabidopsis thaliana and the liverwort, Marchantia polymorpha. (2009)
  • Author(s): Era, A., Tominaga, M., Ebine, K., Awai, C., Saito, C., Ishizaki, K., Yamato, K.T., Kohchi, T., Nakano, A., Ueda, T.
  • Journal Title: Plant Cell Physiol 50 Pages: 1041-1048
  • NAID: 10027340574
  • Peer Reviewed
• [Journal Article] Short actin-based mechanism for light-directed chloroplast movement in Arabidopsis. (2009)
  • Author(s): Kadota, A., Yamada, N., Suetsugu, N., Hirose, M., Saito, C., Shoda, K., Ichikawa, S., Kagawa, T., Nakano, A., Wada, M.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 13106-13111
  • Peer Reviewed
• [Journal Article] Application of Lifeact reveals F-actin dynamics in Arabidopsis thaliana and the liverwort, Marchantia polymorpha (2009)
  • Author(s): Era et al.
  • Journal Title: Plant Cell Physiol 50 Pages: 1041-1048
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission, but have distinct roles in peroxisomal fission (2009)
  • Author(s): Fujimoto et al.
  • Journal Title: Plant J 58 Pages: 388-400
  • Peer Reviewed
• [Journal Article] Short actin-based mechanism for light-directed chloroplast movement in Arabidopsis (2009)
  • Author(s): Kadota et al.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 13106-13111
  • Peer Reviewed
• [Journal Article] The chloroplast protein BPG2 functions in brassinosteroid-mediated post-transcriptional accumulation of chloroplast rRNA (2009)
  • Author(s): Komatsu et al.
  • Journal Title: Plant J 61 Pages: 409-422
  • Peer Reviewed
• [Journal Article] Functions of RAB and SNARE proteins in plant life (2009)
  • Author(s): Saito, Ueda
  • Journal Title: Int Rev Cell Mol Biol 274 Pages: 183-233
• [Journal Article] Live cell imaging reveals the dynamics of two sperm cells during double fertilization in Arabidopsis thaliana
  • Author(s): Hamamura, et al.
  • Journal Title: Current Biology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Loss-of-Function Mutations of Retromer Large Subunit Genes Suppress the Phenotype of an Arabidopsis zig Mutant That Lacks Qb-SNARE VTI11
  • Author(s): Hashiguchi et al.
  • Journal Title: Plant Cell (in press)
  • Peer Reviewed
• [Presentation] 液胞上に生じる複雑な膜構造bulbにを生じる変異体の探索 (2011)
  • Author(s): 齊藤知恵子, 粟井千絵, 木内玲子, 植村知博, 富永基樹, 安部弘, 吉本光希, 森田(寺尾)美代, 上田貴志, 中野明彦
  • Organizer: 日本植物生理学会第52回大会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2011-03-20
• [Presentation] ライブイメージングで明らかにした重複受精における配偶体細胞のダイナミクス (2011)
  • Author(s): 浜村有希, 齊藤知恵子, 金岡雅浩, 佐々木成江, 中野明彦, 東山哲也
  • Organizer: 日本植物生理学会第52回大会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2011-03-20
• [Presentation] ブラシノステロイド情報伝達因子BIL4の細胞内における機能解析 (2011)
  • Author(s): 山上あゆみ, 齊藤知恵子, 中澤美紀, 松井南, 作田正明, 中野明彦, 藤岡昭三, 辻本雅文, 浅見忠男, 中野雄司
  • Organizer: 日本植物生理学会第52回大会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2011-03-20
• [Presentation] TGNに局在するSYP4(Qa-SNAREグループ)が関与する高次機能の解析 (2011)
  • Author(s): 植村知博, 齊藤知恵子, 庄田恵子, 海老根一生, 上田貴志, Schultz-Lefert Paul, 中野明彦
  • Organizer: 日本植物生理学会第52回大会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2011-03-20
• [Presentation] 液胞上に生じる複雑な膜構造bulbに以上を生じる変異体の探索 (2011)
  • Author(s): 齊藤知恵子, 粟井千絵, 木内玲子, 植村知博, 富永基樹, 安部弘, 吉本光希, 森田(寺尾)美代, 上田貴志, 中野明彦
  • Organizer: 日本植物生理学会第52回大会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2011-03-20
• [Presentation] ブラシノステロイド情報伝達突然変異体bil4の原因遺伝子の細胞伸張における機能解析 (2010)
  • Author(s): 山上あゆみ, 齊藤知恵子, 中澤美紀, 松井南, 作田正明, 中野明彦, 藤岡昭三, 辻本雅文, 浅見忠男, 中野雄司
  • Organizer: 植物化学調節学会第45回大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-11-02
• [Presentation] ライブイメージングと電子顕微鏡の両方を用いて明らかにした液胞膜の機能 (2010)
  • Author(s): 齊藤知恵子
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学(春日井)
  • Year and Date: 2010-09-10
• [Presentation] 光変換タンパク質KikGR variantを用いた重複受精機構のライブイメージング解析 (2010)
  • Author(s): 浜村有希, 齊藤知恵子, 宮脇敦史, 中川強, 佐々木成江, 中野明彦, 東山哲也
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学(春日井)
  • Year and Date: 2010-09-09
• [Presentation] Quest for the Biological Significance of"Bulbs" (2010)
  • Author(s): Chieko Saito, Tomohiro Uemura, Motoki Tominaga, Kohki Yoshimoto, Kazuo Ebine, Jun Ito, Takashi Ueda, Hiroshi Abe, Miyo Terao Morita, Masao Tasaka, Akihiko Nakano
  • Organizer: Complex Configuration in Vacuolar Membrane ICAR2010
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-06-08
• [Presentation] Quest for the Biological Significance of "Bulbs", Complex Configuration in Vacuolar Membrane (2010)
  • Author(s): Chieko Saito, Tomohiro Uemura, Motoki Tominaga, Kohki Yoshimoto, Kazuo Ebine, Jun Ito, Takashi Ueda, Hiroshi Abe, Miyo Terao Morita, Masao Tasaka, Akihiko Nakano
  • Organizer: ICAR2010
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-06-08
• [Presentation] 植物ステロイドホルモン生合成阻害剤を用いた植物ケミカルバイオロジーブラシノステロイド情報伝達突然変異体bil4の原因遺伝子の探索と機能解析 (2010)
  • Author(s): 山上あゆみ, 齊藤知恵子, 中澤美紀, 松井南, 作田正明, 中野明彦, 藤岡昭三, 辻本雅文, 浅見忠男, 中野雄司
  • Organizer: 日本ケミカルバイオロジー学会第5回年会
  • Place of Presentation: 慶応大学(東京)
  • Year and Date: 2010-05-18
• [Presentation] 植物ステロイドホルモン生合成阻害剤を用いた植物ケミカルバイオロジー～ブラシノステロイド情報伝達突然変異体bil4の原因遺伝子の探索と機能解析～ (2010)
  • Author(s): 山上あゆみ, 齊藤知恵子, 中澤美紀, 松井南, 作田正明, 中野明彦, 藤岡昭三, 辻本雅文, 浅見忠男
  • Organizer: 日本ケミカルバイオロジー学会第5回年会
  • Place of Presentation: 慶応大学(東京)
  • Year and Date: 2010-05-18
• [Presentation] 植物特異的膜交通におけるミオシンモーターの機能解析 (2010)
  • Author(s): 富永基樹, 安部弘, 齊藤知恵子, 江波和彦, 庄田恵子, 粟井千絵, 植村知博, 上田貴志, 中野明彦
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] 植物特異的膜交通におけるミオシンモーターの機能解析 (2010)
  • Author(s): 富永基樹, 安部弘, 齊藤知恵子, 江波和彦, 庄田恵子, 粟井千絵, 植村知博, 上田貴志, 中野明彦
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 神戸
  • Year and Date: 2010-01-11
• [Presentation] ブラシノステロイド情報伝達突然変異体bil4の原因遺伝子の機能解析 (2009)
  • Author(s): 山上あゆみ, 齊藤知恵子, 中澤美紀, 松井南, 作田正明, 中野明彦, 藤岡昭三, 辻本雅文, 浅見忠男, 中野雄司
  • Organizer: 植物科学調節学会第44回大会
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-30
• [Presentation] 植物特異的膜交通におけるミオシンモーターの機能 (2009)
  • Author(s): 富永基樹, 江波和彦, 安部弘, 齊藤知恵子, 庄田恵子, 粟井千絵, 植村知博, 上田貴志, 中野明彦
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] Bright"bulb"s lighted up on the continuous vacuolar membrane (2009)
  • Author(s): Chieko Saito, Chie Awai, Motoki Tominaga, Tomohiro Uemura, Jun Ito, Hiroshi Abe, Takashi Ueda, Miyo T. Morita, Akihiko Nakano
  • Organizer: seeking for its characteristics and biological significance- Annual Meeting of the American Society of Plant Biologists
  • Place of Presentation: Honolulu
  • Year and Date: 2009-07-20
• [Presentation] Bright "bulb" s lighted up on the continuous vacuolar membrane -seeking for its characteristics and biological significance- (2009)
  • Author(s): Chieko Saito, Chie Awai, Motoki Tominaga, Tomohiro Uemura, Jun Ito, Hiroshi Abe, Takashi Ueda, Miyo T.Morita, Akihiko Nakano
  • Organizer: Annual Meeting of the American Society of Plant Biologists
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2009-07-20
• [Presentation] ライブイメージングによって明らかとなった植物ミオシンメンバーの局在やダイナミクスの多様性 (2009)
  • Author(s): 富永基樹, 安部弘, 齊藤知恵子, 庄田恵子, 粟井千絵, 植村知博, 上田貴志, 中野明彦
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02
• [Remarks] ホームページ等