Analysis of the circadian clock regulation by ubiquitination in Arabidopsis

• Project/Area Number: 21770063
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute of Advanced Industrial Science and Technology (2010); The Institute of Physical and Chemical Research (2009)
• Principal Investigator: FUJIWARA Sumire National Institute of Advanced Industrial Science and Technology, 生物プロセス研究部門, 研究員 (50532131)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: シロイヌナズナ / 概日時計 / 概日リズム / ユビキチン / SUMO

Research Abstract

Circadian rhythms are essential for higher plants to survive in the changing environment. In this study, we investigated the relationships between the circadian clock and the protein modifications such as ubiquitination and other ubiquitin-like modifications. We found the possibility that some ubiquitin ligases and substrate proteins might regulate the time-of-day dependent disease resistance. We also found a novel possible regulation mechanism of organ elongation by clock proteins which are targeted by ubiquitination.

Research Products

• [Journal Article] Photoperiod pathway regulates expression of MAF5 and FLC that encode MADS-box transcription factors of the FLC family in Arabidopsis (2010)
  • Author(s): Sumire Fujiwara, Mayu Nakagawa, Atsushi Oda, Kazuhisa Kato, Tsuyoshi Mizoguchi
  • Journal Title: Plant Biotechnology Volume: 27 Pages: 447-454
  • NAID: 10027702608
  • Peer Reviewed
• [Journal Article] PRR5 regulates nuclear import and subnuclear localization of TOC1 in the Arabidopsis circadian clock (2010)
  • Author(s): Wang L, Fujiwara S, Somers DE
  • Journal Title: EMBO J Volume: 29 Pages: 1903-1915
  • Peer Reviewed
• [Journal Article] SUMO E3 ligase HIGH PLOIDY2 regulates endocycle onset and meristem maintenance in Arabidopsis (2009)
  • Author(s): Ishida T, Fujiwara S, Miura K, Stacey N, Yoshimura M, Schneider K, Adachi S, Minamisawa K, Umeda M, Sugimoto K
  • Journal Title: Plant Cell 21(8) Pages: 2284-2297
  • Peer Reviewed
• [Journal Article] SUMO E3 ligase HIGH PLOIDY2 regulates endocycle onset and meristem maintenance in Arabidopsis (2009)
  • Author(s): Takashi Ishida et al
  • Journal Title: The Plant Cell 21 Pages: 2284-2297
  • Peer Reviewed
• [Journal Article] Thinking outside the F-box : novel ligands for novel receptors (2009)
  • Author(s): David E Somers, Sumire Fujiwara
  • Journal Title: Trends in Plant Science 14 Pages: 206-213
  • Peer Reviewed
• [Presentation] 弱光下で異常な形質を示すシロイヌナズナCRES-T系統の単離・解析 (2011)
  • Author(s): 藤原すみれ、光田展隆、高木優
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-22
• [Presentation] Characterization of A Natural Variation between Ws and Ler That May Affect Expression of Floral Repressors FLC and MAF2-5 in The Clock Mutant 1hy ; cca1 (2011)
  • Author(s): Rim Nefissi、夏井悠、藤原すみれ、鈴木俊二、原美由紀、溝口剛
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-21
• [Presentation] SUMO E3 ligase HIGH PLOIDY2 regulates endocycle onset and meristem maintenance in Arabidopsis (2010)
  • Author(s): Ishida T, Fujiwara S, Miura K, Stacey N, Yoshimura M, Schneider K, Adachi S, Minamisawa K, Umeda M, Sugimoto K
  • Organizer: 日本植物生理学会第51回年会、日本植物生理学会第51回年会
  • Place of Presentation: 熊本
• [Presentation] Novel SUMO E3 ligase HIGH PLOIDY2 regulates endocycle onset and meristem maintenance in Arabidopsis (2010)
  • Author(s): Takashi Ishida et al
  • Organizer: 日本植物生理学会第51回年会
  • Place of Presentation: 熊本大学(熊本)
• [Presentation] Post-translational Regulation of the Arabidopsis Circadian Clock through Selective Proteolysis and Phosphorylation of Pseudo-response Regulator Proteins (2009)
  • Author(s): Fujiwara S, et al
  • Organizer: 9th IPMB Congress
  • Place of Presentation: St.Louis, MO, USA
  • Year and Date: 2009-10-28
• [Presentation] Post-translational Regulation of the Arabidopsis Circadian Clock through Selective Proteolysis and Phosphorylation of Pseudo-response Regulator Proteins (2009)
  • Author(s): Sumire Fujiwara et al
  • Organizer: 9th IPMB Congress
  • Place of Presentation: America's Center(St.Louis, MO, USA)
  • Year and Date: 2009-10-28
• [Book] Two-Component Signaling Systems (Methods in Enzymology, Part C), Characterization of Pseudo-Response Regulators In Plants (Chapter 19) (2010)
  • Author(s): Kim WY, Salome PA, Fujiwara S, Somers DE, McClung CR
  • Publisher: Elsevier
• [Book] Two-Component Signaling Systems, Part C (Methods in Enzymology), Characterization of Pseudo-Response Regulators In Plants(Chapter 19) (2010)
  • Author(s): Woe-Yeon Kim et al
  • Publisher: Elsevier(In press)
• [Book] 「光周性の分子生物学」第3章 植物概日時計の光同調機構 (2009)
  • Author(s): 藤原すみれ(海老原史樹文・井澤毅編)
  • Publisher: シュプリンガー・ジャパン
• [Book] 「光周性の分子生物学」第3章 植物概日時計の光同調機構pp27-37 (2009)
  • Author(s): 藤原すみれ(海老原史樹文・井澤毅編)
  • Total Pages: 11
  • Publisher: シュプリンガー・ジャパン
• [Remarks] ホームページ等
  • URL: http://unit.aist.go.jp/bpri/bpri-grr/bounce.html