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Unraveling regulatory network in wood formation

Research Project

Project/Area Number 21770064
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

MITSUDA Nobutaka  独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究員 (80450667)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords植物 / ゲノム / 発現制御 / 遺伝子 / バイオマス / シロイヌナズナ / 木質 / 転写因子 / 転写制御 / 酵母 / ワンハイブリッド
Research Abstract

New efficient improved yeast one-/two-hybrid screening system has been developed in which the artificial library containing only Arabidopsis genes for transcription factor(TF) is employed. This new system lets me identify TF binding to specific DNA or protein in very efficient manner. I screened TF binding to the regulatory region of genes involving to wood formation and identified many TFs as candidates. This result gradually makes me to draw entire picture of transcriptional regulatory network. Furthermore, I found some TFs rescuing phenotype of wood-less mutant in Arabidopsis and am analyzing their biological functions.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (19 results)

All 2012 2011 2010 2009

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (9 results) Book (2 results)

  • [Journal Article] A NAC domain protein family contributing to the regulation of wood formation in poplar2011

    • Author(s)
      Ohtani, M., Nishikubo, N., Xu B., Yamaguchi, M., Mitsuda, N., and Goue, N., Shi, F., Ohme-Takagi, M., and Demura, T.
    • Journal Title

      Plant J

      Volume: 67 Issue: 3 Pages: 499-512

    • DOI

      10.1111/j.1365-313x.2011.04614.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation2011

    • Author(s)
      Yamaguchi, M., Mitsuda, N., Ohtani, M., Ohme-Takagi, M., Kato, K., and Demura, T
    • Journal Title

      Plant J

      Volume: 66 Issue: 4 Pages: 579-590

    • DOI

      10.1111/j.1365-313x.2011.04514.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] VASCULAR-RELATED NAC-DOMAIN 7 directly regulates the expression of a broad range of genes for xylem vessel formation2011

    • Author(s)
      山口雅利、光田展隆, ほか
    • Journal Title

      PLANT JOURNAL

      Volume: (掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VND-INTERACTING2, a NAC domain transcription factor, negatively regulates xylem vessel formation in Arabidopsis2010

    • Author(s)
      Yamaguchi, M., Ohtani, M., Mitsuda, N., Kubo, M., Ohme-Takagi, M., Fukuda, H., and Demura, T.
    • Journal Title

      Plant Cell

      Volume: 22 Issue: 4 Pages: 1249-1263

    • DOI

      10.1105/tpc.108.064048

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient yeast one-/two-hybrid screening using a library composed only of transcription factors in Arabidopsis thaliana2010

    • Author(s)
      Mitsuda, N., Ikeda, M., Takada, S., Takiguchi, Y., Kondou, Y., Yoshizumi, T., Fujita, M., Shinozaki, K., Matsui, M., and Ohme-Takagi, M.
    • Journal Title

      Plant Cell Physiol

      Volume: 51 Issue: 12 Pages: 2145-2151

    • DOI

      10.1093/pcp/pcq161

    • NAID

      10028056951

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient Yeast One-/Two-Hybrid Screening Using a Library Composed Only of Transcription Factors in Arabidopsis thaliana2010

    • Author(s)
      光田展隆, ほか
    • Journal Title

      PLANT & CELL PHYSIOLOGY

      Volume: 51 Pages: 2145-2151

    • NAID

      10028056951

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VND-INTERACTING2, a NAC Domain Protein, Negatively Regulates Xylem Vessel Formation in Arabidopsis2010

    • Author(s)
      山口雅利、大谷美沙都、光田展隆, ほか
    • Journal Title

      PLANT CELL

      Volume: 22 Pages: 1249-1263

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of transcription factors in Arabidopsis.2009

    • Author(s)
      光田展隆, ほか
    • Journal Title

      Plant and Cell Physiology 50

      Pages: 1232-1248

    • NAID

      10027341408

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 改良酵母ワンハイブリッド法を活用したシロイヌナズナとイネの転写制御ネットワーク解明の試み2012

    • Author(s)
      光田展龍, ほか
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都府)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Comprehensive study of plant transcription factors2011

    • Author(s)
      Nobutaka Mitsuda, et al
    • Organizer
      22nd International Conference on Arabidopsis Research
    • Place of Presentation
      University of Wisconsin(米国マディソン市)
    • Year and Date
      2011-06-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 改良酵母ワンハイブリッド法により転写制御ネットワークを明らかにしようとする試み2011

    • Author(s)
      光田展隆, ほか
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2011-03-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 改良酵母ワンハイブリッド法により転写制御ネットワークを明らかにしようとする試み2011

    • Author(s)
      光田展隆、高木優
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台市
    • Year and Date
      2011-03-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Comprehensive study of plant transcription factors2011

    • Author(s)
      Nobutaka Mitsuda, et al
    • Organizer
      Plant & Animal Genomes XIX Conference
    • Place of Presentation
      米国サンディエゴ市
    • Year and Date
      2011-01-18
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Development of new methods to identify transcription factors that interact with promoter region2010

    • Author(s)
      Nobutaka Mitsuda, et al
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2010-06-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of new methods to identify transcription factors that interact with promoter region2010

    • Author(s)
      光田展隆, ほか
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      横浜市
    • Year and Date
      2010-06-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 上流転写制御因子を同定する新技術の開発2010

    • Author(s)
      光田展龍, ほか
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学(熊本市)
    • Year and Date
      2010-03-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 上流転写制御因子を同定する新技術の開発2010

    • Author(s)
      光田展隆, ほか
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本大学(熊本県)
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Book] Biomass to biofuels, strategies for global industries2011

    • Author(s)
      Nobutaka Mitsuda, et al.
    • Related Report
      2011 Final Research Report
  • [Book] Biomass to biofuels, strategies for global industries2010

    • Author(s)
      光田展隆, ほか
    • Publisher
      WILEY
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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