Unraveling regulatory network in wood formation

• Project/Area Number: 21770064
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: MITSUDA Nobutaka 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究員 (80450667)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 植物 / ゲノム / 発現制御 / 遺伝子 / バイオマス / シロイヌナズナ / 木質 / 転写因子 / 転写制御 / 酵母 / ワンハイブリッド

Research Abstract

New efficient improved yeast one-/two-hybrid screening system has been developed in which the artificial library containing only Arabidopsis genes for transcription factor(TF) is employed. This new system lets me identify TF binding to specific DNA or protein in very efficient manner. I screened TF binding to the regulatory region of genes involving to wood formation and identified many TFs as candidates. This result gradually makes me to draw entire picture of transcriptional regulatory network. Furthermore, I found some TFs rescuing phenotype of wood-less mutant in Arabidopsis and am analyzing their biological functions.

Research Products

• [Journal Article] A NAC domain protein family contributing to the regulation of wood formation in poplar (2011)
  • Author(s): Ohtani, M., Nishikubo, N., Xu B., Yamaguchi, M., Mitsuda, N., and Goue, N., Shi, F., Ohme-Takagi, M., and Demura, T.
  • Journal Title: Plant J Volume: 67 Issue: 3 Pages: 499-512
  • DOI: 10.1111/j.1365-313x.2011.04614.x
  • Peer Reviewed
• [Journal Article] VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation (2011)
  • Author(s): Yamaguchi, M., Mitsuda, N., Ohtani, M., Ohme-Takagi, M., Kato, K., and Demura, T
  • Journal Title: Plant J Volume: 66 Issue: 4 Pages: 579-590
  • DOI: 10.1111/j.1365-313x.2011.04514.x
  • Peer Reviewed
• [Journal Article] VASCULAR-RELATED NAC-DOMAIN 7 directly regulates the expression of a broad range of genes for xylem vessel formation (2011)
  • Author(s): 山口雅利、光田展隆, ほか
  • Journal Title: PLANT JOURNAL Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] VND-INTERACTING2, a NAC domain transcription factor, negatively regulates xylem vessel formation in Arabidopsis (2010)
  • Author(s): Yamaguchi, M., Ohtani, M., Mitsuda, N., Kubo, M., Ohme-Takagi, M., Fukuda, H., and Demura, T.
  • Journal Title: Plant Cell Volume: 22 Issue: 4 Pages: 1249-1263
  • DOI: 10.1105/tpc.108.064048
  • Peer Reviewed
• [Journal Article] Efficient yeast one-/two-hybrid screening using a library composed only of transcription factors in Arabidopsis thaliana (2010)
  • Author(s): Mitsuda, N., Ikeda, M., Takada, S., Takiguchi, Y., Kondou, Y., Yoshizumi, T., Fujita, M., Shinozaki, K., Matsui, M., and Ohme-Takagi, M.
  • Journal Title: Plant Cell Physiol Volume: 51 Issue: 12 Pages: 2145-2151
  • DOI: 10.1093/pcp/pcq161
  • NAID: 10028056951
  • Peer Reviewed
• [Journal Article] Efficient Yeast One-/Two-Hybrid Screening Using a Library Composed Only of Transcription Factors in Arabidopsis thaliana (2010)
  • Author(s): 光田展隆, ほか
  • Journal Title: PLANT & CELL PHYSIOLOGY Volume: 51 Pages: 2145-2151
  • NAID: 10028056951
  • Peer Reviewed
• [Journal Article] VND-INTERACTING2, a NAC Domain Protein, Negatively Regulates Xylem Vessel Formation in Arabidopsis (2010)
  • Author(s): 山口雅利、大谷美沙都、光田展隆, ほか
  • Journal Title: PLANT CELL Volume: 22 Pages: 1249-1263
  • Peer Reviewed
• [Journal Article] Functional analysis of transcription factors in Arabidopsis. (2009)
  • Author(s): 光田展隆, ほか
  • Journal Title: Plant and Cell Physiology 50 Pages: 1232-1248
  • NAID: 10027341408
  • Peer Reviewed
• [Presentation] 改良酵母ワンハイブリッド法を活用したシロイヌナズナとイネの転写制御ネットワーク解明の試み (2012)
  • Author(s): 光田展龍, ほか
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-16
• [Presentation] Comprehensive study of plant transcription factors (2011)
  • Author(s): Nobutaka Mitsuda, et al
  • Organizer: 22nd International Conference on Arabidopsis Research
  • Place of Presentation: University of Wisconsin(米国マディソン市)
  • Year and Date: 2011-06-22
• [Presentation] 改良酵母ワンハイブリッド法により転写制御ネットワークを明らかにしようとする試み (2011)
  • Author(s): 光田展隆, ほか
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2011-03-21
• [Presentation] 改良酵母ワンハイブリッド法により転写制御ネットワークを明らかにしようとする試み (2011)
  • Author(s): 光田展隆、高木優
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-03-21
• [Presentation] Comprehensive study of plant transcription factors (2011)
  • Author(s): Nobutaka Mitsuda, et al
  • Organizer: Plant & Animal Genomes XIX Conference
  • Place of Presentation: 米国サンディエゴ市
  • Year and Date: 2011-01-18
• [Presentation] Development of new methods to identify transcription factors that interact with promoter region (2010)
  • Author(s): Nobutaka Mitsuda, et al
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2010-06-08
• [Presentation] Development of new methods to identify transcription factors that interact with promoter region (2010)
  • Author(s): 光田展隆, ほか
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: 横浜市
  • Year and Date: 2010-06-08
• [Presentation] 上流転写制御因子を同定する新技術の開発 (2010)
  • Author(s): 光田展龍, ほか
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本市)
  • Year and Date: 2010-03-20
• [Presentation] 上流転写制御因子を同定する新技術の開発 (2010)
  • Author(s): 光田展隆, ほか
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-20
• [Book] Biomass to biofuels, strategies for global industries (2011)
  • Author(s): Nobutaka Mitsuda, et al.
• [Book] Biomass to biofuels, strategies for global industries (2010)
  • Author(s): 光田展隆, ほか
  • Publisher: WILEY