| Project/Area Number |
21770069
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphology/Structure
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| Research Institution | University of Toyama |
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Principal Investigator |
KONNO Norifumi University of Toyama, 大学院・理工学研究部, 助教 (50507051)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ツメガエル / 発生 / 腎臓 / 体液調節 / 比較生理学 / 前腎 / 両生類 / メダカ |
|---|
| Research Abstract |
To understand integrative renal functions in vertebrates, we explored and characterized genes expressing in pronephros of amphibian tadpole and mesonephros of teleost. We constructed the GFP expression vector linked 5'-upstream promoter of Na^+, K^+-ATPase, marker protein of renal tubule, and succeeded in isolating a living pronephric tubule by using transgenic technique. Furthermore, we explored genes associated with polycystic kidney disease (PKD) by a PCR-based subtractive hybridization method using pc mutant, a PKD model of medaka. As a result, we identified 2 genes (vasotocin type-2 receptor and aquaporin-8) reduced markedly in the kidney of pc mutant. Currently, we explore genes expressing in pronephros by global analysis and then characterize 2 genes found as candidate gene associated with teleost PKD.
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